检索结果-南通市图书馆

Plant Diversity 2024年第3期46卷 386-394页

作者： Yunzhen Li Liujie Jin Xinyu Liu Chao He Siteng Bi Sulaiman Saeed Wenhao Yan National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China

vernalization is necessary for winter wheat to ***,it is unclear whether vernalization is also required for spring wheat,which is frequently sown in fall,and what molecular mechanisms underlie the vernalization response in wheat *** this study,we examined the molecular mechanisms that regulate vernalization response in winter and spring wheat *** this purpose,we determined how major vernalization genes(VRN1,VRN2,and VRN3)respond to vernalization in these varieties and whether modifications to histones play a role in changes in gene *** also identified genes that are differentially regulated in response to vernalization in winter and spring wheat *** found that in winter wheat,but not in spring wheat,VRN1 expression decreases when returned to warm temperature following *** finding may be associated with differences between spring and winter wheat in the levels of tri-methylation of lysine 27 on histone H3(H3K27me3)and tri-methylation of lysine 4 on histone H3(H3K4me3)at the VRN1 *** of winter wheat transcriptomes before and after vernalization revealed that vernalization influences the expression of several genes,including those involved in leucine catabolism,cysteine biosynthesis,and flavonoid *** findings provide new candidates for further study on the mechanism of vernalization regulation in wheat.

关键词： Wheat vernalization VRN1 Histone modification Regulate network

Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 1995年第7期38卷 799-806页

作者：种康谭克辉黄华梁梁厚果 Department of Biology Lanzhou UniversityLanzhou 730000China Institute of Botany Chinese Academy of SciencesBeijing 100044China Institutc of Genetics Chinese Academy of SciencesBeijing 100101China

A cDNA clone related to the vernalization in winter wheat(verc203)was harvested from the en-riched cold-induced cDNA library of 10~4 pfu with differential *** insert of verc203 in λ gt10 vector wassubcloned into the sites between BamH Ⅰ and Hind Ⅲ in pUC19 plasmid after being amplified with *** analysis of the Northern blotting with a probe of verc203 indicated that the verc203 has a negative signalfor the control and the devernalized mRNA and a positive signal for the vernalized winter wheat and non-vernalized spring wheat at about 2.6 kb.

关键词： winter wheat vernalization gene molecular cloning of cDNA.

Effect of vernalization on tuberization and flowering in the Tibetan turnip is associated with changes in the expression of FLC homologues

在线全文

学校读者我要写书评

暂无评论

Plant Diversity 2018年第2期40卷 50-56页

作者： Yan Zheng Landi Luo Yuanyuan Liu Yunqiang Yang Chuntao Wang Xiangxiang Kong Yongping Yang Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 China Germplasm Bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 China University of Chinese Academy of Sciences Beijing 100049 China School of Ecology and Environmental Science Yunnan University Kunming 650504 China

The turnip(Brassica rapa var. rapa) is a biennial crop that is planted in late summer/early fall and forms fleshy tubers for food in temperate regions. The harvested tubers then overwinter and are planted again the next spring for flowering and seeds. FLOWERING LOCUS C(FLC) is a MADS-box transcription factor that acts as a major repressor of floral transition by suppressing the flowering promoters FT and SOC1. Here we show that vernalization effectively represses tuber formation and promotes flowering in Tibetan turnip. We functionally characterized four FLC homologues(BrrFLC1,FLC2, FLC3, and FLC5), and found that BrrFLC2 and BrrFLC1 play a major role in repressing flowering in turnip and in transgenic Arabidopsis. In contrast, tuber formation was correlated with BrrFLC1 expression in the hypocotyl and was repressed under cold treatment following the quantitative downregulation of BrrFLC1. Grafting experiments of non-vernalized and vernalized turnips revealed that vernalization independently suppressed tuberization in the tuber or hypocotyl of the rootstock or scion, which occurred in parallel with the reduction in BrrFLC1 activity. Together, our results demonstrate that the Tibetan turnip is highly responsive to cold exposure, which is associated with the expression levels of BrrFLC genes.

关键词： Tibetan turnip Tuberization Flowering BrrFLC genes vernalization

The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2021年第9期14卷 1525-1538页

作者： Shujuan Xu Qi Dong Min Deng Dexing Lin Jun Xiao Peilei Cheng Lijing Xing Yuda Niu Caixia Gao Wenhao Zhang Yunyuan Xu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Innovation Academy for Seed Design Chinese Academy of SciencesBeijing 100093China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory of Plant Cell and Chromosome Engineering Center for Genome EditingInstitute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China College of Horticulture Nanjing Agricultural UniversityKey Laboratory of LandscapingMinistry of AgricultureNanjing 210095China CAS-JIC Centre of Excellence for Plant and Microbial Science Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

vernalization is a physiological process in which prolonged cold exposure establishes flowering competence in winter plants. In hexaploid wheat, TaVRN1 is a cold-induced key regulator that accelerates floral transition. However, the molecular mechanism underlying the gradual activation of TaVRN1 during the vernalization process remains unknown. In this study, we identified the novel transcript VAS (TaVRN1 alternative splicing) as a non-coding RNA derived from the sense strand of the TaVRN1 gene only in winter wheat, which regulates TaVRN1 transcription for flowering. VAS was induced during the early period of vernalization, and its overexpression promoted TaVRN1 expression to accelerate flowering in winter wheat. VAS physically associates with TaRF2b and facilitates docking of the TaRF2b-TaRF2a complex at the TaVRN1 promoter during the middle period of vernalization. TaRF2b recognizes the Sp1 motif within the TaVRN1 proximal promoter region, which is gradually exposed along with the disruption of a loop structure at the TaVRN1 locus during vernalization, to activate the transcription of TaVRN1. The tarf2b mutants exhibited delayed flowering, whereas transgenic wheat lines overexpressing TaRF2b showed earlier flowering. Taken together, our data reveal a distinct regulatory mechanism by which a long non-coding RNA facilitates the transcription factor targeting to regulate wheat flowering, providing novel insights into the vernalization process and a potential target for wheat genetic improvement.

关键词： winter wheat vernalization flowering long non-coding RNA chromosome loop

The Relationship Between Developmental Accumulation of Leaf Soluble Proteins and vernalization Response of Wheat(Triticum aestivum ***. Thell)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2009年第4期8卷 410-417页

作者： M Majdi G Karimzadeh S Mahfoozi Plant Breeding and Biotechnology Department Faculty of AgricultureTarbiat Modares UniversityP.O.Box 14115-336Tehran ***Iran Physiology-Agronomy Unit of Department of Cereals Research Seed and Plant Improvement InstituteKaraj 315854119Iran

The relationship between vernalization requirement and quantitative and qualitative changes in total leaf soluble proteins were determined in one spring （cv. Kohdasht） and two winter （cvs. Sardari and Norstar） cultivars of wheat （Triticum aestivum L.） exposed to 4℃. Plants were sampled on days 2, 14, 21 and 35 of exposure to 4℃. The final leaf number （FLN） was determined throughout the vernalization periods （0, 7, 14, 24, and 35 d） at 4℃. The final leaf number decreased until days 24 and 35 in Sardari and Norstar eultivars, respectively, indicating the vernalization saturation at these times. No clear changes were detected in the final leaf number of Kohdash cultivar, verifying no vernalization requirement for this spring wheat cultivar. Comparing with control, clear cold-induced 2-fold increases in proteins quantity occurred after 48 h following the 4℃-treatment in the leaves of the both winter wheat cultivars but, such response was not detected in the spring cultivar. However, the electrophoretic protein patterns showed between-cultivar and between-temperature treatment differences. With increasing exposure time to 4℃, the winter cultivars tended to produce more HMW polypeptides than the spring cultivar. Similar proteins were induced in both Sardari and Norstar winter wheat cultivars, however, the long vernalization requirement in Norstar resulted in high level and longer duration of expression of cold-induced proteins compared to Sardari with a short vernalization requirement. These observations indicate that vernalization response regulates the expression of low temperature （LT） tolerance proteins and determines the duration of expression of LT- induced proteins.

关键词： soluble proteins wheat Triticum aestivum L. final leaf number vernalization cold

MiR396 is involved in plant response to vernalization and flower development in Agrostis stolonifera

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 380-393页

作者： Shuangrong Yuan Zhigang Li Ning Yuan Qian Hu Man Zhou Junming Zhao Dayong Li Hong Luo Department of Genetics and Biochemistry Clemson University110 Biosystems Research ComplexClemsonSC 29634USA Department of Grassland Science Sichuan Agricultural University611130 ChengduSichuanChina Beijing Vegetable Research Center Beijing Academy of Agriculture and forestry Science100097 BeijingChina

MicroRNA396(miR396)has been demonstrated to regulate flower development by targeting growth-regulating factors(GRFs)in annual ***,its role in perennial grasses and its potential involvement in flowering time control remain *** we report that overexpression of miR396 in a perennial species,creeping bentgrass(Agrostis stolonifera L.),alters flower *** significantly,transgenic(TG)plants bypass the vernalization requirement for *** expression analysis reveals that miR396 is induced by long-day(LD)photoperiod and *** study identifies VRN1,VRN2,and VRN3 homologs whose expression patterns in wild-type(WT)plants are similar to those observed in wheat and barley during transition from short-day(SD)to LD,and SD to cold ***,compared to WT controls,TG plants overexpressing miR396 exhibit significantly enhanced VRN1 and VRN3 expression,but repressed VRN2 expression under SD to LD conditions without vernalization,which might be associated with modified expression of methyltransferase ***,our results unveil a potentially novel mechanism by which miR396 suppresses the vernalization requirement for flowering which might be related to the epigenetic regulation of VRN genes and provide important new insight into critical roles of a miRNA in regulating vernalization-mediated transition from vegetative to reproductive growth in monocots.

关键词： flower creep vernalization

Effect of vernalization and plant growth regulators on flowering of Onithogalum ‘Chesapeake Snowflake’

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2006年第1期7卷 23-28页

作者：王锦 Virginia R.Walter 西南林学院园林学院 Horticulture and Crop Science Department California Polytechnic State UniversitySan Luis Obispo CA 93407USA

The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regulator treatment, chilling bulbs for 3 weeks at 10℃ before planting accelerated flowering of the first inflorescence by 5 to 6 days, elongated floral stem length by 1.5 to 2 cm and shortened leaf length by 4 to 5 cm as compared with non-chilled bulbs. When bulbs were chilled 3 weeks at 10℃ before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 2 to 6 days respectively as compared with the control. When bulbs were not chilled before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 6 to12 days respectively as compared with the control. The lengths of the leaves and the inflorescences were not affected by PGR treatment. Higher rates of Pro-Gibb （100 mg.L^-1 and 200 mg.L^-1） and more amount of Fascination 2%（100 μL and 200 μL） can cause abortion of inflorescence.

关键词： vernalization plant growth regulators flowering Onithogalum ‘Chesapeake Snowflake'

Effects of Different Low Overwintering Temperatures on Angelica vernalization and Premature Bolting

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2018年第4期19卷 55-62页

作者： JIA Zhen DI Sheng-qiang ZHAO Fei-yi LI San-xiang WANG Shun-cai College of Bioengineer and Technology Institute of Plant Functional Genomics in Southeast GansuTianshui Normal UniversityTianshui 741000PRC College of Horticultural Science and Engineering Shandong Agricultural UniversityTai’an 271018PRC

Angelica sinensis(Oliv.)Diels is a valuable herbal *** production,its premature bolting,caused by vernalization,seriously affects its root *** this stuty,thermal treatments of 5,0 and?5°C,as well as treatments with shifts between these temperatures,were performed in its overwintering storage to investigate the chilling effect on the early bolting of Angelica sinensis and analyze the vernalization response to different *** results indicated that the 0°C treatment rendered the highest bolting rate,and the-5°C resulted in the lowest bolting *** initial short-term treatment at-5°C could promote the early bolting of ***,and the treatment at 5°C prior to planting could hasten its seedling bud *** the appropriate temperature,the early bolting rate of Angelica was positively related to the number of treatment *** the frozen storage at-5°C could inhibit the vernalization of Angelica seedlings and the early *** vernalization of Angelica could be a continuous process related to the number of treatment *** study would serve as reference for the overwintering storage and cultivation of Angelica.

关键词： Angelica sinensis vernalization Premature bolting Thermal treatment

Study on the Change of GAs,ABA Level and ABA/GAs Ratio in the Embryos of Spring Wheat and Winter Wheat during vernalization

在线全文

学校读者我要写书评

暂无评论

Journal of Northeast Agricultural University(English Edition) 1995年第2期2卷 156-159页

作者： Mi Guohua, Li Wenxong(Northeast Agricultural University, Harbin 150030 PRC)

Spring wheat (cv. 7742) and winter wheat (cv. Nongda 015) were vernalized by seed chilling, and the changes of GAs, ABA content and GAs / ABA ratio in embryo were investigated. The differences between cultivars, treatments and variety-treatment interactions were significant for both GAs and ABA. The level of GAs and ABA as well as GAs / ABA ratio were hardly changed by seed chilling in spriug wheat However, in winter wheat, the GAs content decreased much less compared with ABA that reduced sharply after chilling. It showed that the GAs / ABA ratio in winter wheat was increased remarkably (4.55 times) than in spring wheat. It was suggested that the absolute GAs level was independent of the vernalization effect because there was a higher GAs level before and after seed chilling in winter wheat when compared with spring wheat, One of the vemalization effects was the decline of ABA level that led to the increase of GAs / ABA ratio.

关键词： ABA GAs vernalization wheat

Molecular epigenetic understanding of flowering-time regulation by vernalization in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Molecular epigenetic understanding of flowering-time regulat...