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histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery

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Neural Regeneration Research 2022年第4期17卷 832-837页

作者： Yi Shen Ming-Jiang Yao Yu-Xin Su Dong-Sheng Xu Jia Wang Guang-Rui Wang Jing-Jing Cui Jian-Liang Zhang Wan-Zhu Bai Institute of Acupuncture and Moxibustion China Academy of Chinese Medical SciencesBeijingChina Institute of Basic Medical Sciences Xiyuan Hospital of China Academy of Chinese Medical SciencesBeijingChina Beijing Key Laboratory of Pharmacology of Chinese Materia Medica BeijingChina

The mouse model of multiple cerebral infarctions,established by injecting fluorescent microspheres into the common carotid artery,is a recent development in animal models of cerebral ischemia.To investigate its effectiveness,mouse models of cerebral infarction were created by injecting fluorescent microspheres,45–53μm in diameter,into the common carotid artery.Six hours after modeling,fluorescent microspheres were observed directly through a fluorescence stereomicroscope,both on the brain surface and in brain sections.Changes in blood vessels,neurons and glial cells associated with microinfarcts were examined using fluorescence histochemistry and immunohistochemistry.The microspheres were distributed mainly in the cerebral cortex,striatum and hippocampus ipsilateral to the side of injection.Microinfarcts were found in the brain regions where the fluorescent microspheres were present.Here the lodged microspheres induced vascular and neuronal injury and the activation of astroglia and microglia.These histopathological changes indicate that this animal model of multiple cerebral infarctions effectively simulates the changes of various cell types observed in multifocal microinfarcts.This model is an effective,additional tool to study the pathogenesis of ischemic stroke and could be used to evaluate therapeutic interventions.This study was approved by the Animal Ethics Committee of the Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences(approval No.D2021-03-16-1)on March 16,2021.

关键词： astrocytes blood-brain barrier common carotid artery fluorescent microsphere histochemistry ischemia microglia microinfarcts neuron neurovascular unit stroke

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The 12th China-Japan Joint Seminar on histochemistry and Cytochemistry

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中国组织化学与细胞化学杂志 2017年第4期26卷 403-428页

01 Differentiation-dependent changes in DNA methylation and histone H3 acetylation: possible roles of these epigenetic factors in mouse spermatogenesis Takehiko Koji Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan Mammalian spermatogenesis is a well-ordered process, consisting of spermatogonial mitosis, spermatocytic meiosis and spermiogenesis.The process is also characterized by successive chromatin condensation and a high frequency of spermatogenic cell apoptosis.Therefore, epigenetic factors can be implicated in the regulation of spermatogenesis and any epigenetic disorder may lead to the induction of apoptosis. When we analyzed global level of DNA methylation by immunohistochemistry with anti-5-methylcytosine in paraffin-embedded sections of mouse testis, there was no difference among any stages of germ cells.

关键词： Cytochemistry histochemistry Cell Biology

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High-efficiency somatic embryogenesis and morphohistology and histochemistry of somatic embryo development in Larix leptolepis Gordon

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Forestry Studies in China 2007年第3期9卷 182-188页

作者： Wang Xiao-xiong Lu Long-dou Hao Huai-qing Teng Nian-jun Chen Tong Guo Yi-ming Yang Ying-gen Guo Zhong-chen Lin Jin-xing Key Laboratory of Photosynthesis and Molecular Environmental Physiology Institute of Botany the Chinese Academy of Sciences Beijing 100093 P. R. China College of Life Sciences Henan Normal University Xinxiang 453007 P. R. China

A high-efficiency somatic embryogenesis protocol of Japanese larch （Larix leptolepis Gordon） has been established in our investigation. Calli were induced from immature zygotic embryos of female cones ofL. leptolepis and then subcultured regularly on to a modified Gupta and Durzan （DCR） basal medium for 5 years. Embryogenic tissues showed distinct morphological changes dur- ing somatic embryo development when they were transferred to a maturation medium supplemented with abscisic acid （ABA） com- pared with the morphology in a medium lacking ABA. Histological observations indicated that polyembryony was a characteristic feature during early embryogeny and somatic embryos at later stages showed normal histodifferentiation. In addition, histochemical analysis revealed that abundant starch granules and proteins accumulated in mature embryos, indicating that they played important roles in the development and regeneration of normal plantlets from somatic embryos on hormone-free germination media

关键词： Larix leptolepis Gordon abscisic acid （ABA） histochemistry morphohistology polyembryony

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ACETYLCHOLINESTERASE histochemistry OF THE THALAMUS IN THE PRIMATE

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Academic Journal of Xi'an Jiaotong University 2001年第2期13卷 101-106页

作者：刘健张巧俊 Department of Physiology Med. School of Xi'an Jiaotong Univ. Xi' an 710061 China

Objective To observe the distribution of acetylcholinesterase activity in the thalamus of the monkey.Methods Histochemical method was used to detect the acetylcholinesterase activity in the thalamus.Results Acetylcholinesterase was found to be inhomogeneous distribution in the primate thalamus and to reveal previously uncovered inhomogeneity within certain thalamic nuclei and their subdivisions. The medial, ventral and posterior nuclear groups displayed markedly uneven acetylcholinesterase reaction.In the mediodorsal nucleus,three distinct sbudivisions were revealed by acetylcholinesterase histochemistry, medial magnocellular part, ventral sector of central parvicellular part and dorsolateral sector of lateral pars multiformity showed weak, moderate and strong acetylcholinesterase activity, respectively. In the ventral nuclear group, acetylcholinesterase histochemistry was strong in the medial part of ventral posterior nucleus, moderate in the magnocellular part of ventral anterior, caudal, medial, oral and pars postrema parts of ventral lateral nucleus, as well as lateral part of ventral posterior nucleus, poor and weak in the inferior part of ventral posterior nucleus, par compacta of the medial part of ventral posterior nucleus and parvicellular part of ventral anterior nucleus. In the pulvinar nucleus, acetylcholinesterase reaction ranged from weak, moderate to strong in the parts of the oral, medial and lateral, as well as inferior of this nucleus, respectively. Regional variations of acetylcholinesterase activity within the thalamic nuclei and their subdivisions can help to identify them by acetylcholinesterase histochemistry. In addition, the dark patches of strong acetylcholinesterase activity contrasting with a lighter surrounding matrix were revealed within the parvicellular part and pars multiformis of mediodorsal nucleus, paracentral nucleus, central lateral nucleus, pars postrema part of ventral lateral nucleus and medial habenula nucleus, as well as medial part of pulvinar necleus, respectively.Conc

关键词： mediodorsal nucleus ventral thalamic nuclei acetylcholinesterase histochemistry thalamus monkey

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Enzymatic　histochemistry　of　Retina　with　Experimental　Intraocular　Pressure　Elevation　in　Rabbits

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1995年第3期15卷 171-175页

作者：杨智宽杜蜀华刘勇熊希凯 Department of Ophthalmology Tongji Hospital Tongji Medical University Wuhan

To investigate the pathogenesis of retina lesions caused by intraocular pressure elevation, activities and distribution of enzymes in retina including lactic dehydrogenase (LDH), succinate dehydrogenase (SDH), adenosinetriphosphatase (ATPase), acid phosphatase (ACP), cholinesterase (ChE), cytochrome oxidase(CCO),nucleotidase (5'-Nase) and glucose-6-phosphatase (G6Pase) were determined histochemically in 30 rabbits. It was found that 1) in the early stage of intraocular pressure elevation, the activities of LDH, SDH, ATPase, ACP, and ChE in retina were increased, while the activities of CCO,5'-Nase decreased;2)in thelate stage of intraocular pressure elevation, the activities of all these enzymes but ACP, which showed a reduced activity, were close to the normal level; 3) in superoxide dismutase.(SOD-CCE) treated group, except the slight increase of LDH and G6Pase activities,the activities of the remaining enzymes were near to normal. Our results suggest that the various histochemical changes in retina induced by intraocular pressure elevation were cornpensatory in the early stage and were beneficial to the supply of energy needed in retinal tissue andcellular metabolism;while in the late stage, the lesion of retina cells developed due to decompensation.SOD-CCE could alleviate the retinal lesions caused by intraocular pressure elevation, and can be used as auxiliary drug for the treatment of intraocular pressure elevation.

关键词： retina intraocular pressure elevation histochemistry superoxide dismutase

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A Study of Histology and Enzymatic histochemistry on Rabbit's Retina in Acute Ocular Hypertension

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眼科学报 1994年第3期10卷 144-150页

作者： Weirong Sun, Shumei Wang, Guiqiu Zhao, Jianlu Gao, Fangyi LiuThe Department of Ophthalmology, The Affilliated Hospital of Qingdao Medical College Qingdao 266003, China Department of Ophthalmology Affilliated Hospital of Qingdao Medical College China.

The changes of activities of enzymes relating to energy metabolism in rabbit's retina in acute ocular hypertension were observed. The activities of succinate dehydrogenase and adenosine triphosphatase were found to be reduced, while the activities of the lactatic dehydrognease and glucose-6-phosphatase increased. The results revealed the metabolic disturbance of energy in retina after acute ocular hypertension might be the underlying factors relating to the defects of the functions and structures of the...

关键词： acute ocular hypertension retina enzymes histochemistry

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Effects of Natural Radiation, PAR and Artificial Ultraviolet Radiation-B on the Ultrastructure and histochemistry of Leaf of Oryza sativa L.

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American Journal of Plant Sciences 2012年第10期3卷 1361-1368页

作者： Sérgio L. de Almeida éder C. Schmidt Ana C. Rodrigues Zenilda L. Bouzon Central Laboratory of Electron Microscopy Federal University of Santa Catarina Florianópolis Brazil Department of Botany Federal University of Santa Catarina Florianópolis Brazil Post-Graduate Program in Cell Biology and Development Department of Cell Biology Embryology and Genetics Federal University of Santa Catarina Florianópolis Brazil

Ultraviolet radiation-B (UVBR) affects plants in many important ways, including reduced growth rate, reduction of primary productivity and changes in ultrastructure. The rice (Oryza sativa) is one of the most cultivated cereal in the world along with corn and wheat, representing over 50% of agricultural production. In this study, we examined O. sativa exposed to natural radiation denominated which “ambient samples”, plants cultivated which photosynthetically active radiation (PAR), denominated with PAR-only and plants cultivated with PAR + UVBR for 2 h per day during 30 days of cultivation in vitro. The samples were processed for electron microscopy and histochemistry analysis. PAR + UVBR caused changes in the ultrastructure of leaf of O. sativa, mesophyll cells, which included increased thickness of the cell wall and plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast and mitochondria internal organization. The exposure to PAR + UVBR led to changes in guard and subsidiary cells, and the stomata and papillae were with irregular shape. The reduction of epicuticular wax that covered the leaf, was observed. Taken together, these ?ndings strongly suggested that PAR + UVBR negatively affects the ultrastructure and morphology and growth rates, of leaf of O. sativa and, in the long term, their economic viability.

关键词： Oryza sativa Ultraviolet Radiation-B histochemistry Ultrastructure

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Black soldier fly and gut health in broiler chickens:insights into the relationship between cecal microbiota and intestinal mucin composition

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Journal of Animal Science and Biotechnology 2020年第3期11卷 841-852页

作者： Ilaria Biasato Ilario Ferrocino Sihem Dabbou Rocchina Evangelista Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone Department of Agricultural Forest and Food SciencesUniversity of TurinLargo Paolo Braccini 210095 GrugliascoTOItaly Department of Veterinary Sciences University of TurinLargo Paolo Braccini 210095 GrugliascoTOItaly Institute of Science of Food Production National Research CouncilLargo Paolo Braccini 210095 GrugliascoTOItaly

Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now well recognized)on gut microbiota and mucin composition have recently been reported in Tenebrio molitor-fed free-range and broiler chickens,but no data are currently available for Hermetia illucens(HI)-fed broilers.The present study evaluated the effects of dietary HI meal inclusion on cecal microbiota and intestinal mucin composition of broiler chickens.Results:A total of 256 male broiler chickens were allotted to 4 dietary treatments(control diet[C]and 5%,10%and 15%HI meal inclusion,with 8 replicate pens/treatment and 8 birds/pen)and slaughtered at 35 d of age(2 animals/pen,16 birds/diet).The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed lower alpha diversity in HI15 chickens(Shannon,P<0.05)and higher beta diversity(Adonis and ANOSIM,P<0.001)in birds fed HI diets than C.Furthermore,HI15 birds displayed significant increase of the relative abundance of Proteobacteria phylum(False Discovery Rate[FDR]<0.05)when compared to HI10.L-Ruminococcus(Ruminococcus from Lachnospiraceae family),Faecalibacterium,Blautia and Clostridium genera were found to be characteristic of HI5 cecal microbiota(FDR<0.05),while broiler chickens fed HI10 and HI15 diets were characterized(FDR<0.05)by Lactobacillus and Ruminococcus(HI10)and Bacteroides,Roseburia and Helicobacter genera(HI15).Periodic-acid Schiff,Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine also demonstrated lower mucin staining intensity in the intestinal villi of HI10 and HI15 birds than C(P<0.05).Conclusions:Dietary HI meal utilization at low inclusion levels(i.e.,5%)positively influenced either the cecal microbiota or the gut mucin dynamics in terms of selection of potentially beneficial bacteria and increase in villi mucins.However,high inclusion levels(in particular the 15%)may have a negative influenc

关键词： 16S rRNA Gut health Hermetia illucens L. histochemistry Insect meal Microbiota Mucin Poultry

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The prevention role of Spirulina platensis(Arthrospira platensis)on intestinal health

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Food Science and Human Wellness 2022年第5期11卷 1342-1346页

作者： Ender Deniz Asmaz Nilay Seyidoglu Department of Histology and Embryology Veterinary FacultyBursa Uludag UniversityNilufer 16059Turkey Department of Physiology Veterinary FacultyTekirdag Namik Kemal UniversitySuleymanpasa 59030Turkey

Spirulina is a popular herbal food that has a preventive effect on health.In the study,it aimed to evaluate the effects of different doses of Spirulina platensis(Arthrospira platensis)on the morphological properties of the duodenum of rats,and to assign the effect of Spirulina on the expression of proliferating cell nuclear antigen(PCNA)in the rat duodenum,and thereby to observe the effects on intestinal health.30 male Wistar albino rats were divided into 3 groups for 45 days.The first group was received the basal diet;the second group was given 500 mg/kg Spirulina daily by oral gavage;the third group was given 1000 mg/kg Spirulina daily.The duodenum segments were taken at the end of the trial and processed for histological assay.Although the total mucosa,villus height and villus/cript ratio were found higher in high dose Spirulina,the lower cript depth was detected in same group compared to control and low dose group.A significant increase was observed at high dose compared to the control and low dose group in terms of PCNA expression intensity and proliferation index.Findings suggest that high dose of Spirulina may support the duodenal growth,and thereby intestinal health.

关键词： histochemistry Duodenum Spirulina platensis Intestinal health

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新疆石河子地区116例少数民族及汉族正常成人骨髓象及组织化学染色结果分析

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农垦医学 1985年第3期 162-167页

作者：廖永葆顾秀田刘林顺石河子医学院附属医院

本文观察了116例新疆少数民族及汉族正常成人骨髓象及6项组化染色的情况。结果表明少数民族与汉族骨髓细胞分类及计数200个与500个细胞结果无显著差异,但男性组中红及红系总数均高于女性组,两者有显著差异。本文的中杆值及晚红值低于天... 详细信息

本文观察了116例新疆少数民族及汉族正常成人骨髓象及6项组化染色的情况。结果表明少数民族与汉族骨髓细胞分类及计数200个与500个细胞结果无显著差异,但男性组中红及红系总数均高于女性组,两者有显著差异。本文的中杆值及晚红值低于天津的报告,其它比例与文献基本一致。还提出可将 POX、NAE 及ANAE 的阳性率作为估计骨髓中粒细胞、单核细胞及 T 细胞总数的参考。并发现民、汉两组的细胞内铁明显低于文献值,民族组的内铁及淋巴细胞的 PAS 结果又低于汉族组。

关键词： histochemistry rubricyte erythrocyte neutrophil metarubricyte POX NAE ANAE PAS granulocyte monocyte normoblast lymphocyte

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