High-efficiency somatic embryogenesis and morphohistology and histochemistry of somatic embryo development in Larix leptolepis Gordon

作     者：Wang Xiao-xiong Lu Long-dou Hao Huai-qing Teng Nian-jun Chen Tong Guo Yi-ming Yang Ying-gen Guo Zhong-chen Lin Jin-xing 

作者机构：Key Laboratory of Photosynthesis and Molecular Environmental Physiology Institute of Botany the Chinese Academy of Sciences Beijing 100093 P. R. China College of Life Sciences Henan Normal University Xinxiang 453007 P. R. China 

出 版 物：《Forestry Studies in China》 (中国林学（英文版）)

年 卷 期：2007年第9卷第3期

页      面：182-188页

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0907[农学-林学] 08[工学] 0829[工学-林业工程] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：the National Natural Science Foundation of China （Grant No. 30471366） the National Science Fund of China for Distinguished Young Scholars （30225005）. 

主　　题：Larix leptolepis Gordon abscisic acid （ABA） histochemistry morphohistology polyembryony 

摘      要：A high-efficiency somatic embryogenesis protocol of Japanese larch （Larix leptolepis Gordon） has been established in our investigation. Calli were induced from immature zygotic embryos of female cones ofL. leptolepis and then subcultured regularly on to a modified Gupta and Durzan （DCR） basal medium for 5 years. Embryogenic tissues showed distinct morphological changes dur- ing somatic embryo development when they were transferred to a maturation medium supplemented with abscisic acid （ABA） com- pared with the morphology in a medium lacking ABA. Histological observations indicated that polyembryony was a characteristic feature during early embryogeny and somatic embryos at later stages showed normal histodifferentiation. In addition, histochemical analysis revealed that abundant starch granules and proteins accumulated in mature embryos, indicating that they played important roles in the development and regeneration of normal plantlets from somatic embryos on hormone-free germination media