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Diagnostic and monitoring values of circulating AFPmRNA and HS-GGT for hepatocellular carcinoma

The Chinese-German Journal of Clinical Oncology 2014年第10期13卷 457-463页

作者： Li Wang Min Yao Yao Yao Liuhong Pan Shaolin Lu Dengfu Yao Research Center of Clinical Medicine Affiliated Hospital of Nantong University Medical School of Nantong University The Hospital of Nantong Maternal and Child Care Service Center of Oncology Affiliated Hospital of Nantong University

Objective: Early finding of hepatocellular carcinoma(HCC) and monitoring of its metastasis are of the utmost importance. The objectives of this study were to investigate the values of circulating hepatoma-specific gamma-glutamyl transferase(HS-GGT) fraction and α-fetoprotein mRNA(AFP-mRNA) from peripheral blood mononuclear cells(PBMCs) in diagnosis or monitoring metastasis of HCC. Methods: Total RNA was extracted from hepatomas or PBMCs of patients, synthesized to AFP-cDNA through random primers and reverse transcriptase, amplified by using a nested PCR assay, and confirmed by sequencing. Simultaneity, the HS-GGT activities were quantitatively determined in sera of patients. The comprehensive assessments of two markers were investigated in HCC patients. Results: The amplified fragments of AFP-mRNA were identical to original designed ones(159 bp) and confirmed by sequencing. The lowest sensitivity was 2 ng/L of total RNA. The incidences of AFP-mRNA and HS-GGT were 60.4%(113 of 187) and 84.5%(158 of 187) in the HCC group, and their significantly higher(P specific markers for HCC diagnosis or differentiation, especially the former for early finding and the later for monitoring metastasis of HCC.

关键词： hepatocellular carcinoma(HCC) AFP-mRNA hepatoma-specific gamma-glutamyl transferase diagnosis metastasis

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