Diagnostic and monitoring values of circulating AFPmRNA and HS-GGT for hepatocellular carcinoma

作     者：Li Wang Min Yao Yao Yao Liuhong Pan Shaolin Lu Dengfu Yao 

作者机构：Research Center of Clinical Medicine Affiliated Hospital of Nantong University Medical School of Nantong University The Hospital of Nantong Maternal and Child Care Service Center of Oncology Affiliated Hospital of Nantong University 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2014年第13卷第10期

页      面：457-463页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by grants from the National Natural Science Foundation of China(No.81200634) the Projects of Jiangsu Medical Science(No.WSW-011,HK201102,BL2012053) the Qinglan Project of Jiangsu higher Education he International S&T Cooperation Program of China(No.2013DFA32150) 

主　　题：hepatocellular carcinoma(HCC) AFP-mRNA hepatoma-specific gamma-glutamyl transferase diagnosis metastasis 

摘      要：Objective: Early finding of hepatocellular carcinoma(HCC) and monitoring of its metastasis are of the utmost importance. The objectives of this study were to investigate the values of circulating hepatoma-specific gamma-glutamyl transferase(HS-GGT) fraction and α-fetoprotein mRNA(AFP-mRNA) from peripheral blood mononuclear cells(PBMCs) in diagnosis or monitoring metastasis of HCC. Methods: Total RNA was extracted from hepatomas or PBMCs of patients, synthesized to AFP-cDNA through random primers and reverse transcriptase, amplified by using a nested PCR assay, and confirmed by sequencing. Simultaneity, the HS-GGT activities were quantitatively determined in sera of patients. The comprehensive assessments of two markers were investigated in HCC patients. Results: The amplified fragments of AFP-mRNA were identical to original designed ones(159 bp) and confirmed by sequencing. The lowest sensitivity was 2 ng/L of total RNA. The incidences of AFP-mRNA and HS-GGT were 60.4%(113 of 187) and 84.5%(158 of 187) in the HCC group, and their significantly higher(P 0.01) than that in any of other groups. The high frequencies of HS-GGT and AFP-mRNA were 78.2% and 56.4% in the HCC patients with AFP 50 ng/mL. All positive of AFP-mRNA was found in HCC patients with extrahepatic metastasis. No significant correlation was found between tumor size and AFP-mRNA or HS-GGT. The total incidence of combined AFP-mRNA and HS-GGT detection was 93.6 % for HCC diagnosis. Conclusion: HS-GGT and AFP-mRNA are specific markers for HCC diagnosis or differentiation, especially the former for early finding and the later for monitoring metastasis of HCC.