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heat-shock protein 90 in Candida albicans

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Chinese Science Bulletin 2000年第6期45卷 481-484页

作者： Li Wang Xiaojuan Zhu NE Normal Univ Inst Cytol & Genet Changchun 130024 Peoples R China

Researches on Candidal heat-shock protein 90 (HSP90) in recent years are summarized. Candida albicans is a commensal pathogen in human and animals. in immunocompromised individuals it behaves as an opportunist pathogen, giving rise to superficial or systemic infections. Systemic candidosis is a common cause of death among immunocompromised and debilitated patients, in which the mortality is as high as 70%. HSP90 is now recognized as an immunodominant antigen in C. albicans and plays a key role in systemic candidosis as a molecular chaperone. The 47-ku peptide is the breakdown product of HSP90. Patients who has recovered from systemic candidosis produce high titre of antibodies to 47-ku antigen, whereas the fatal cases have little antibody or falling titre? The three commonest epitopes of candidal HSP90 have been mapped, epitopes C, B and H. Epitopes C and H are immunogenic. The antibody probes of both epitopes may be developed into a new serological test agents for systemic candidosis due to rather

关键词： Candida albicans heat-shock protein 90 epitope.

Hepatitis C virus inhibitor synergism suggests multistepinteractions between heat-shock protein 90 and hepatitis Cvirus replication

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World Journal of Hepatology 2016年第5期8卷 282-290页

作者： Naoko Kubota Masataka Nomoto Gi-Wook Hwang Toshihiko Watanabe Michinori Kohara Takaji Wakita Akira Naganuma Shusuke Kuge Laboratory of Molecular and Biochemical Toxicology Graduate School of Pharmaceutical SciencesTohoku UniversityMiyagi 980-8578Japan Department of Microbiology Tohoku Pharmaceutical UniversityMiyagi 981-8558Japan Department of Microbiology and Cell Biology Tokyo Metropolitan Institute of Medical ScienceTokyo 156-0057Japan Department of Virology II National Institute of Infectious DiseasesTokyo 162-8640Japan

AIM: To address the effect of heat-shock protein 90(HSP90) inhibitors on the release of the hepatitis C virus(HCV), a cell culture-derived HCV(JFH1/HCVcc) from Huh-7 cells was ***: We quantified both the intracellular and extracellular(culture medium) levels of the components(RNA and core) of JFH-1/HCVcc. The intracellular HCV RNA and core levels were determined after the JFH1/HCVcc-infected Huh-7 cells were treated with radicicol for 36 h. The extracellular HCV RNA and core protein levels were determined from the medium of the last 24 h of radicicol treatment. To determine the possible role of the HSP90 inhibitor in HCV release, we examined the effect of a combined application of low doses of the HSP90 inhibitor radicicol and the RNA replication inhibitors cyclosporin A(Cs A) or interferon. Finally, we statistically examined the combined effect of radicicoland Cs A using the combination index(CI) and graphical representation proposed by Chou and ***: We found that the HSP90 inhibitors had greater inhibitory effects on the HCV RNA and core protein levels measured in the medium than inside the cells. This inhibitory effect was observed in the presence of a low level of a known RNA replication inhibitor(Cs A or interferon-α). Treating the cells with a combination of radicicol and cyclosporin A for 24 h resulted in significant synergy(CI < 1) that affected the release of both the viral RNA and the core protein. CONCLUSION: In addition to having an inhibitory effect on RNA replication, HSP90 inhibitors may interfere with an HCV replication step that occurs after the synthesis of viral RNA, such as assembly and release.

关键词： Hepatitis C virus Inhibition of hepatitis Cvirus release Cell culture-derived hepatitis C virus heat-shock protein 90 inhibitors Hepatitis C virus RNAreplication

Effects of heat shock on change of HSC70/HSP68,acid and alkaline phosphatases before and after rat partial hepatectomy

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World Journal of Gastroenterology 2000年第5期6卷 730-733页

作者： Lu AL Xu CS Henan Normal Univ Coll Life Sci Xinxiang 453002 Henan Province Peoples R China

INTRODUCTIONOnly the liver has the great capability ofregeneration in *** hepatocytes are inthe phase of division in the normal liver of an adultmammal (including human beings),but theremaining hepatocytes can be induced to proliferatequickly by partial hepatectomy (PH),and,to somedegree,they stop dividing and re-differentiate intocells functioning as *** shows

关键词： partial hepatectomy(PH) liver regeneration conserved heat-shock protein 70/induced heat-shock protein 68(HSCT0/HSP68) acid phosphatases alkaline phosphatases

热休克蛋白90α在人胃癌组织中的表达及其意义

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中华实验外科杂志 2008年第7期25卷 878-880页

作者：吴文辉肖隆斌蔡世荣詹文华王昭中山大学附属第一医院黄埔院区普外科广州510700 中山大学附属第一医院胃肠外科

目的观察热休克蛋白90α（HSP90α）在人胃癌组织和正常胃黏膜组织中的表达，探讨其表达与胃癌的发生、发展及生物学行为的关系。方法收集46例胃癌组织和相应的正常胃黏膜组织标本，分别应用免疫组织化学技术中链霉菌抗生物素蛋白-过氧... 详细信息

目的观察热休克蛋白90α（HSP90α）在人胃癌组织和正常胃黏膜组织中的表达，探讨其表达与胃癌的发生、发展及生物学行为的关系。方法收集46例胃癌组织和相应的正常胃黏膜组织标本，分别应用免疫组织化学技术中链霉菌抗生物素蛋白-过氧化物酶连接法（即SP法）和逆转录-聚合酶链反应（RT-PCR）法检测HSP90α的表达情况，分析检测结果。结果HSP90α在胃癌组织中表达的阳性率为82．6％（38／46），明显高于正常胃黏膜组织中的表达（10／46，21．7％）；在不同分化类型的胃癌组织中，各组间HSP90α阳性表达率差异无统计学意义（P〉0．05）；在淋巴结转移为N1期以后（不包括N1在内）的病例中HSP90α表达阳性率为96．2％（25／26），高于淋巴结转移为N0和N1的病例；在TNM分期Ⅲ、Ⅳ期阳性表达率为92．9％（26／28），也要高于Ⅰ、Ⅱ期。结论HSP90α在胃癌组织中呈现高表达，并且在淋巴结转移多和越晚期的胃癌组织中表达越高，提示HSP90α可作为胃癌预后判定监测指标。

关键词： Stomach neoplasms heat-shock protein Immunohistochemistry

Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae

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Frontiers of Chemical Science and Engineering 2022年第7期16卷 1079-1089页

作者： Jiaxi Lin Zhen Yao Xiaomei Lyu Lidan Ye Hongwei Yu Institute of Bioengineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311200China Key Laboratory of Systems Microbial Biotechnology Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China School of Food Science and Technology Jiangnan UniversityWuxi 214122China

Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter PSSA4 to control the expression of PGAL-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products.

关键词： transcriptional activator directed evolution dynamic control heat-shock isoprene

A New Duplex Reverse Transcription PCR for Simultaneous Detection of Viable Cryptosporidium parvum Oocysts and Giardia duodenalis Cysts

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Biomedical and Environmental Sciences 2010年第2期23卷 146-150页

作者： SEHEE NAM GYUCHEOL LEE Water Analysis and Research Center K-water Daejeon 306-711 Republic of Korea

Objective To simultaneously detect viable Cryptosporidium parvum oocysts and Giardia duodenalis cysts for the purpose of reducing time and cost spent. Methods A duplex reverse transcription polymerase chain reaction （RT-PCR） method was newly developed. Results Using duplex RT-PCR method for the hsp70 gene, viable （oo）cyst concentrations of 10^1 and l0^3 （oo）cysts/100 *** could be detected for C. parvum and G duodenalis, respectively. However, after heat-shock stimulation the expression of hsp70 mRNAs was detectable at 10^0 and 10^1 （oo）cysts/100 μL concentrations of C. parvum and G. duodenalis, respectively. Thus, the detection sensitivity was significantly increased when the viable （oo）cysts were exposed to heat shock. Conclusion This study describes a new duplex RT-PCR method for hsp70 gene to detect the viable （oo）cysts of the C. parvum and G duodenalis with less time consumed and at a lower cost. This newly developed duplex RT-PCR method may be used to detect these parasites not only in aquatic environments but also in clinical samples.

关键词： Cryptosporidiumparvum Giardia duodenalis heat-shock RT-PCR

Role of TRP channels and HSPs in thermal stress response in the aphid parasitoid Aphelinus asychis(Hymenoptera:Aphelinidae)

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Journal of Integrative Agriculture 2020年第6期19卷 1530-1542页

作者： LIU Xiang KANG Zhi-wei YU Xing-lin LI Fan LIU Tong-xian LI Qiang Department of Entomology College of Plant ProtectionYunnan Agricultural UniversityKunming 650000P.R.China State Key Laboratory of Crop Stress Biology for Arid Areas/Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture and Rural Affairs Northwest A&F UniversityYangling 712100P.R.China

Aphelinus asychis is an important aphid *** field and greenhouse conditions,high temperature is one of the factors limiting the application of A asychis for biological pest *** explore the potential role of transient receptor potential(TRP)channels and heat shock proteins(HSPs)in this process,we identified 11 genes encoding TRP channels and nine genes encoding *** proteins(AasyTRPA5,AasyPyrexia,AasyPainless)that belong to transient receptor potential ankyrin(TRPA)subfamily and nine HSPs are involved in the response to high *** also investigated the survival of A asychis and the response of the identified TRP channels and HSPs to high *** results showed that the maximum temperature that allowed *** survival was approximately 41°C;females had higher survival rates than that of the males at 40 and 41°***-term heat-shock resulted in increased expression of Amsysbsp in males,and Aasyhsp40,Aasyhsp68,Aasyhsp70-4,Aasyhsp70-5 and Aasyhsp90 were upregulated and then down regulated,whereas Aasyhsp70-3was upregulated at41°***,Aasyhsp40and Aasyhsp90showed higherexpression levels in females,while Aasyshsp and Aasyhsp70-3 presented opposite expression *** temperature above 35°C,expression of AasyPyrexia in females was significant higher than that in males,whereas AasyPainless mnd AasyTRPA5 presented higher expression in males at 40 and 41°C,***,these results indicate that protect!on against thermal stress in *** is coordinated by TRP channels and *** findings provide a basis for understanding the potential mechanism of A asychis in response to high temperatures.

关键词： Aphelinus asychis TRP channels heat-shock proteins thermal stress gene expression

Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe

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Journal of Genetics and Genomics 2019年第9期46卷 423-432页

作者： Jing Zhang Ji-Long Liu MRC Functional Genomics Unit Department of PhysiologyAnatomy and GeneticsUniversity of OxfordOxford0X13PTUnited Kingdom School of Life Science and Technology ShanghaiTech UniversityShanghai201210China

The metabolic enzyme CTP synthase(CTPS) is able to compartmentalize into filaments,termed cytoophidia,in a variety of organisms including bacteria,budding yeast,fission yeast,fruit flies and mammals.A previous study in budding yeast shows that the filament-forming process of CTPS is not sensitive to temperature *** we study CTPS filamentation in the fission yeast Schizosaccharomyces *** our surprise,we find that both the length and the occurrence of cytoophidia in *** decrease upon cold shock or heat *** temperature-dependent changes of cytoophidia are fast and *** advantage of yeast genetics,we demonstrate that heat-shock proteins are required for cytoophidium assembly in *** sensitivity of cytoophidia makes *** an attractive model system for future investigations of this novel membraneless organelle.

关键词： CTP synthase Cytoophidium Schizosaccharomyces pombe heat-shock protein Nucleoside/nucleotide metabolism Cell biology Yeast genetics Cell compartmentalization

Mechanisms of Action of Noninvasive Monopolar Radiofrequency: Technology Review

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Open Journal of Orthopedics 2013年第1期3卷 23-28页

作者： Terry L. Whipple Scott P. Steinmann Department of Orthopaedics Mayo Clinic Rochester USA. Orthopaedic Research of Virginia Richmond USA

Tendinosis is now understood as the result of a failed tendon healing process regardless of where it occurs in the body. Current noninvasive therapeutic alternatives are anti-inflammatory in nature and outcomes are unpredictable at best. The benefit of invasive alternatives resides in the induction of the healing response as demonstrated in pre-clinical and clinical studies in cardiology and orthopaedics. A new technology that employs noninvasivemonopolar capacitive-coupled radiofrequency (mcRF), has demonstrated the ability to raise temperatures in tendons and ligaments upwards of 50°C, the temperature threshold for collagen modulation and recruitment of macrophages, fibroblasts and heat shock Protein factors—without damaging the overlying structures—resulting in activation of the Wound Healing Response (WHR).

关键词： Thermal Shrinkage Thermoplasty heat-shock AT2 Radiofrequency Collagen Denaturation Tendinosis Wound Healing Response Tendinopathy Microtenotomy Antinociceptive