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Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) in rice

Journal of Genetics and Genomics 2009年第2期36卷 117-123页

作者： Tao Chen Yadong Zhang Ling Zhao Zhen Zhu Jing Lin Suobing Zhang Cailin Wang Institute of Food Crops Jiangsu Academy of Agricultural Sciences Jiangsu High Quality Rice Research and Development Center Nanjing 210014 China

green-revertible albino is a novel type of chlorophyll deficiency in rice （Oryza sativa L.）, which is helpful for further research in chlorophyll synthesis and chloroplast development to illuminate their molecular mechanism. In the previous study, we had reported a single recessive gene, gra（t）, controlling this trait on the long arm of chromosome 2. In this paper, we mapped the gra（t） gene using 1,936 recessive individuals with albino phenotype in the F2 population derived from the cross between themo-photoperiod-sensitive genic male-sterile （T/PGMS） line Pei＇ai 64S and the spontaneous mutant Qiufeng M. Eventually, it was located to a confined region of 42.4 kb flanked by two microsatellite markers RM2-97 and RM13553. Based on the annotation results of RiceGAAS system, 11 open reading frames （ORFs） were predicted in this region. Among them, ORF6 was the most possible gene related to chloroplast development, which encoded the chloroplast protein synthesis elongation factor Tu in rice. Therefore, we designated it as the candidate gene of gra（t）. Sequence analysis indicated that only one base substitution C to T occurred in the coding region, which caused a missense mutation （Thr to Ile） in gra（t） mutant. These results are very valuable for further study on gra（t） gene.

关键词： green-revertible albino fine mapping gra(t) Oryza sativa L.

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Deletion of a Dna K protein gene causes seedling green-revertible albino by retarding chloroplast development in rice

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Science Bulletin 2015年第23期60卷 2054-2058页

作者： Weitao Li Junjie Yin Bangquan Ye Chunfang Peng Qinshu Cheng Jing Wang Can Yuan Heng Yin Zhixiong Chen Min He Yuping Wang Weilan Chen Jichun Wang Bingtian Ma Peng Qin Shigui Li Xuewei Chen Rice Research Institute Key Laboratory of Major Crop DiseasesState Key Laboratory of Hybrid Rice and Collaborative Innovation Center for Hybrid Rice in Yangtze River BasinSichuan Agricultural University

green-revertible albino mutants are important sources for studying chloroplast structure, chloroplast development, chlorophyll biosynthesis, and plant photo- synthesis. In the present study, we characterized a green- revertible albino mutant gra（k）, which was obtained from the tissue-cultured rice Kitaake. The mutant gra（k） exhib- ited albino on its first three leaves. The leaf color started to turn green at the four-leaf stage. The chlorophyll contents were deeply reduced at the seedling stage, and the chloroplast development was delayed in gra（k）. The green- revertible albino （gra） phenotype of the mutant gra（k） was temperature dependent. The main agronomic traits, including plant height, tilling number per plant, seed set- ting rate, and thousand-grain weight, slightly decreased in gra（k） comparing to those in the wild-type Kitaake. Genetic analysis showed that the gra phenotype was con- trolled by a single recessive nucleic gene. By using 5,168 recessive F2 individuals derived from the cross of gra（k） × Jodan, the locus of the gene Gra（k） was delimited in a DNA region of 200 kb between the makers B-31 and P11 on chromosome 5. Sequencing analysis indicated that the three functionally annotated genes, LOC_Os05g23700, LOC_Os05g23720, and LOC_Os05g23740, were all deleted in the 200 kb region in the mutant gra（k）. Trans- genic test revealed that the gra（k） plants over-expressing LOC_Os05g23740CDS were restored to normal green as the wild-type Kitaake. Our results proved that the deletion of the DnaK protein gene LOC_Os05g23740 （encoding the chaperon protein OsHsp70CP1） led to the gra phenotype in the mutant gra（k）.

关键词： Rice green-revertible albino Agronomic character - Chlorophyll content Finemapping

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