Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) in rice

作     者：Tao Chen Yadong Zhang Ling Zhao Zhen Zhu Jing Lin Suobing Zhang Cailin Wang 

作者机构：Institute of Food Crops Jiangsu Academy of Agricultural Sciences Jiangsu High Quality Rice Research and Development Center Nanjing 210014 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2009年第36卷第2期

页      面：117-123页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National High-tech Research and Development Program of China (863 Program) (No. 2006AA100101) the National Science and Technology Support Program (No. 2006BAD01A01-5) Special Program for Rice Scientific Research of Ministry of Agriculture (No. nyhyzx 07-001-006) Super Rice Breeding and Demonstration Program of Ministry of Agriculture, and Jiangsu Selfinnovation Fund for Agricultural Science and Technology (No. CX603) 

主　　题：green-revertible albino fine mapping gra(t) Oryza sativa L. 

摘      要：Green-revertible albino is a novel type of chlorophyll deficiency in rice （Oryza sativa L.）, which is helpful for further research in chlorophyll synthesis and chloroplast development to illuminate their molecular mechanism. In the previous study, we had reported a single recessive gene, gra（t）, controlling this trait on the long arm of chromosome 2. In this paper, we mapped the gra（t） gene using 1,936 recessive individuals with albino phenotype in the F2 population derived from the cross between themo-photoperiod-sensitive genic male-sterile （T/PGMS） line Pei＇ai 64S and the spontaneous mutant Qiufeng M. Eventually, it was located to a confined region of 42.4 kb flanked by two microsatellite markers RM2-97 and RM13553. Based on the annotation results of RiceGAAS system, 11 open reading frames （ORFs） were predicted in this region. Among them, ORF6 was the most possible gene related to chloroplast development, which encoded the chloroplast protein synthesis elongation factor Tu in rice. Therefore, we designated it as the candidate gene of gra（t）. Sequence analysis indicated that only one base substitution C to T occurred in the coding region, which caused a missense mutation （Thr to Ile） in gra（t） mutant. These results are very valuable for further study on gra（t） gene.