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Effects of Crude Enzyme Preparation Supplemented to Barley Diet on the Performance, Hormone Levels and Biochemical Parameters in gosling

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Journal of Shanghai Jiaotong university(Science) 2005年第S1期10卷 115-119页

作者：艾晓杰 School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China

Twenty four 7-day-old crossbred goslings （Chuan white×Tai lake） were divided randomly into control and treatment groups. Enzyme preparation was added in the diet of treatment group at dosage of 1 g/kg. The blood samples were collected from 60 day-old goslings via the wing vein. During the fifty-three days experiment, body weights were adopted measured each week, related hormone contents and biochemical parameters in plasma were determined. The results were as following:（1） Compared with control, body weight of treatment group were significantly higher （Pgoslings. The weight gains were significant higher （Pgoslings too, feed intake were increased and the "feed: body weight gain" was decreased; （2） Compared with control, growth hormone （GH） and insulin-like growth factor-I （IGF-I） levels were dramatically enhanced by 36.62% （Pgosling growth by improving the barely digestibility, carbohydrate metabolism and influencing the hormone levels.

关键词： enzyme preparation barley gosling performance hormones biochemical parameters

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“戴着眼镜的蜘蛛”James gosling访谈

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程序员 2003年第11期 22-26页

作者：熊建国孟岩

大约八年前的今天,Java刚刚诞生后不久,《经济学家》在一篇题为“Will your next computer be a tin can and a wire?”的报道中画了一副有趣的漫画,一个人怀抱着一台电脑,电脑的一端连着一条线,线的另一端是一张网络(蜘蛛网),蜘蛛网上... 详细信息

大约八年前的今天,Java刚刚诞生后不久,《经济学家》在一篇题为“Will your next computer be a tin can and a wire?”的报道中画了一副有趣的漫画,一个人怀抱着一台电脑,电脑的一端连着一条线,线的另一端是一张网络(蜘蛛网),蜘蛛网上蹲坐着一只硕大无比的“戴着一副眼镜的蜘蛛”。今天,这只“蜘蛛”就笑眯眯的坐在我们面前,他就是Java之父James gosling。

关键词： Java语言程序员软件开发 James gosling

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Simple and Rapid Method for Detection of Goose Parvovirus in the Field by Loop-Mediated Isothermal Amplification

Simple and Rapid Method for Detection of Goose Parvovirus in...

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第五届中国畜牧科技论坛

作者： Yang Jin-long Yang Rui Cheng An-chun Wang Ming-shu Fu Li-zhi Yang Song-quan Zhang Su-hui Yang Liu Xu Zhi-yong Avian Diseases Research Center College of Veterinary Medicine of Sichuan Agricultural University Chongqing Academy of Animal Science Key Laboratory of Animal Diseases and Human Health of Sichuan Province College of Animal Sciences Henan Institute of Science and Technology

Background:Goose parvovirus(GPV) is a Dependovirus associated with latent infection and mortality in ***,it severely affects geese production *** objective of this study was to develop a loop-mediated isothermal amplification(LAMP) method for sensitive,rapid and inexpensive detection of GPV in the ***:A set of six specific primers was designed by targeting the GPV VP3 DNA. With Bst DNA polymerase large fragment,the target DNA could be amplified at 65℃as early as 20 min incubation in a simple water bath.A positive reaction was identified by the detection of the LAMP product by color change visible to the naked *** detection limit of the assay was 28 copies/μl of plasmid pVP3,with equally sensitivity and specificity detection by fluorescent quantitative real-time PCR(FQ-PCR).Conclusions: The high sensitivity,specificity,and simplicity,with no complicated equipment and technical training of the GPV LAMP assay,combined with a high throughput,make this method suitable for a broad spectrum both in field condition and in laboratory operation for specific detection of GPV infected.

关键词： GPV LAMP gosling real-time PCR

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Development of a rapid and ultrasensitive real-time PCR assay for detection and quantification of goose parvovirus

Development of a rapid and ultrasensitive real-time PCR assa...

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第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛

作者： Jin-Long Yang An-Chun Cheng Ming-Shu Wang Kang-Cheng Pan Min Li Xue-Feng Qi Xin-Feng Han De-Kang Zhu Xiao-Yue Chen (Avian Diseases Research Center,College of Veterinary Medicine of Sichuan Agricultural University,Yaan 625014,Sichuan China Key Laboratory of Animal Diseases and Human Health of Sichuan Province,Yaan 625014,Sichuan Province,China Research Center of Animal Microecological Engineering,Sichuan Agricultural University,Yaan,Sichuan,625014,P.R.China WEN’S GROUP,Guangzhou,510000,Guangdong Province,China)

It is important for controling gosling plague effectively that detecting Goose parvovirus rapidly and *** the present article,a real-time PCR assay was developed for the diagnosis of Goose *** method was established and optimized through the primer and probe designed according to the gene sequence of Goose parvovirus *** detection limit of the assay was found as little as 0.2 fg (28 copies) of the DNA,the sensitivity of the assay was compared with conventional PCR technique and 1000 times more than the conventional PCR ***,it was compared with conventional PCR and virus neutralization assay in detecting of clinical infected goslings,the positive percentage based on the real-time PCR was 100%,the conventional PCR was 78.8-90.9%,and the virus neutralization assay was 63.6-81.8%.This assay has the potential to be used for the rapid and specific clinical diagnostic purposes particularly for the detection and quantitation of low-grade Goose parvovirus infections.

关键词： Real-time PCR GPV gosling ultrasensitive VP3

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向数字技术老爹致敬

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微电脑世界 2004年第12期 6-6页

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基于Java的网络版JQ开发心得

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艺术科技 2016年第12期29卷 70-页

作者：聂称心杜月莹吉璇沈阳理工大学辽宁沈阳110159

Java语言诞生于1991年,起初他只是Sun公司为自己旗下的一些消费性电子产品所设计的通用环境,在那个年代,硬件领域出现了单片式计算机系统,这种价格低廉的系统一出现就立即引起了自动控制领域人员的注意。Sun公司研发人员根据嵌入式软件... 详细信息

Java语言诞生于1991年,起初他只是Sun公司为自己旗下的一些消费性电子产品所设计的通用环境,在那个年代,硬件领域出现了单片式计算机系统,这种价格低廉的系统一出现就立即引起了自动控制领域人员的注意。Sun公司研发人员根据嵌入式软件的要求对C++进行了改造,去除了留在C++的一些不太实用及影响安全的成分,并结合嵌入式系统的实时性要求,开发了Oak面向对象语言,Java的前身诞生了。经过对oak语言的一系列修改,Java在James gosling手中诞生了。

关键词： Java James gosling 面向对象 Oak

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