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Adhesive ginsenoside compound k patches for cartilage tissue regeneration

Regenerative Biomaterials 2023年第1期10卷 1442-1450页

作者： Jun-Ho Yang Hyun Ho Shin Donghyeon kim Ji Hyun Ryu Eun-Jung Jin Department of Biological Sciences College of Health SciencesWonkwang UniversityIksanJeonbuk 54538South Korea Department of Chemical Engineering Wonkwang UniversityIksanJeonbuk 54538South Korea Department of Carbon Convergence Engineering Wonkwang UniversityIksanJeonbuk 54538South Korea Integrated Omics Institute Wonkwang UniversityIksanJeonbuk 54538South Korea

Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery *** instance,compound k(Ck)is a major metabolite of ginsenosides that is known to protect against joint degeneration by inhibiting the production of inflammatory cytokines and the activation of immune ***,its effects on cartilage degradation and tissue regeneration remain ***,tissue-adhesive drug delivery depots that stably adhere to cartilage defects are required for Ck *** this study,Ck-loaded adhesive patches were reported to seal cartilage defects and deliver Ck to defect sites,preventing cartilage degradation and accelerating cartilage tissue *** patches are stable and suitable for application in surgical procedures under physiological conditions and show excellent adhesiveness to cartilage *** addition,there were no significant differences in the adhesive polymeric networks before and after Ck ***-loaded hydrocaffeic acid-conjugated chitosan patches significantly inhibited the stimulation of cartilage-degrading enzymes and apoptosis in osteoarthritic cartilage by releasing Ck in cartilage ***,the NFkB signaling pathway of released Ck from the adhesive patches in the treatment of osteoarthritis is ***,the Ck-loaded adhesive patches are expected to significantly contribute to cartilage regeneration.

关键词： ginsenoside compound k adhesive patch drug delivery hydrocaffeic acid-conjugated chitosan osteoarthritis

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Determination of ginsenoside compound k in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts

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Acta Pharmaceutica Sinica B 2015年第5期5卷 461-466页

作者： Yunhui Chen Youming Lu Yong Yang Xiaoyan Chen Liang Zhu Dafang Zhong Guangdong College of Pharmacy Shanghai Institute of Materia Medica Chinese Academy of Sciences

ginsenoside compound k(GCk), the main metabolite of protopanaxadiol constituents of Panax ginseng, easily produces alkali metal adduct ions during mass spectrometry particularly with lithium. Accordingly, we have developed a rapid and sensitive liquid chromatography–tandem mass spectrometric method for analysis of GCk in human plasma based on formation of a lithium adduct. The analyte and paclitaxel(internal standard) were extracted from 50 m L human plasma using methyl tertbutyl ether. Chromatographic separation was performed on a Phenomenex Gemini C18 column(50 mm 2.0 mm; 5 μm) using stepwise gradient elution with acetonitrile–water and 0.2 mmol/L lithium carbonate at a flow rate of 0.5 m L/min. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions at m/z 629-449 for the GCk-lithium adduct and m/z 860-292 for the adduct of paclitaxel. The assay was linear in the concentration range 1.00–1000 ng/m L(r^2>40.9988)with intra- and inter-day precision of ±8.4% and accuracy in the range of 4.8% to 6.5%. Recovery,stability and matrix effects were all satisfactory. The method was successfully applied to a pharmacokinetic study involving administration of a single GCk 50 mg tablet to healthy Chinese volunteers.

关键词： LC MS/MS ginsenoside compound k Lithium adduct ion Pharmacokinetics

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Biotransformation differences of ginsenoside compound k mediated by the gut microbiota from diabetic patients and healthy subjects

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Chinese Journal of Natural Medicines 2023年第10期21卷 723-729页

作者： HUANG Sutianzi SHAO Li CHEN Manyun WANG Lin LIU Jing ZHANG Wei HUANG Weihua Department of Clinical Pharmacology Xiangya HospitalCentral South UniversityChangsha 410008China Institute of Clinical Pharmacology Hunan Key Laboratory of PharmacogeneticsCentral South UniversityChangsha 410078China The First Affiliated Hospital of Shantou University Medical College Shantou 515041China Department of Pharmacognosy School of PharmacyHunan University of Chinese MedicineChangsha 410128China

Many natural products can be bio-converted by the gut microbiota to influence pertinent *** compound k(GCk)is a potential anti-type 2 diabetes(T2D)saponin,which is mainly bio-transformed into protopanaxadiol(PPD)by the gut *** have shown that the gut microbiota between diabetic patients and healthy subjects are significantly ***,we aimed to characterize the biotransformation of GCk mediated by the gut microbiota from diabetic patients and healthy *** on 16S rRNA gene sequencing,the results indicated the bacterial profiles were considerably different between the two groups,especially Alistipes and Parabacteroides that increased in healthy *** quantitative analysis of GCk and PPD showed that gut microbiota from the diabetic patients metabolized GCk slower than healthy subjects through liquid chromatography tandem mass spectrometry(LC-MS/MS).The selected strain *** and *** exhibited a different metabolic capability of *** conclusion,the different biotransformation capacity for GCk may impact its anti-diabetic potency.

关键词： ginsenoside compound k Gut microbiota Biotransformation Diabetes

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Transformation of compound k from Saponins in Leaves of Panax notoginseng by Immobilized β-Glucanase

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Chinese Herbal Medicines 2010年第1期2卷 -页

作者： DONG Hui-juan1,2,JIANG Bin-hui3,HAN Ying3,GENG Yong1,ZHAO Yu-qing4 1.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China 2.Graduate School of Chinese Academy of Sciences,Beijing 100039,China 3.School of Resources and Civil Engineering,Northeastern University,Shenyang 110004,China 4.Shenyang Pharmaceutical University,Shenyang 110016,China Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China Graduate School of Chinese Academy of Sciences Beijing 100039 China School of Resources and Civil Engineering Northeastern University Shenyang 110004 China Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China Shenyang Pharmaceutical University Shenyang 110016 China

Objective To prepare an active anti-tumor component,compound k(C-k),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-*** Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-*** amount and purity of C-k obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were *** β-Glucanase can be immobilized and reused with either of the ***,using Alg 1 resulted in higher enzyme activity than Alg *** optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4–6 h;and the optimal concentration of the crosslinking agent was 6%– 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-k from SLPN.

关键词： β-glucanase ginsenoside compound k immobilization saponins in leaves of Panax notoginseng(Burk.) F.H.Chen transformation

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