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Biosafety and Health 2023年第5期5卷 259-265页

作者： Jia Wan Xiaomin Zhang Jing Qu Bo Peng Dongfeng Kong Jianhua Lu Qinghua Hu Zhifeng Zhou Haiduan Lin Xiangjie Yao Yulin Fu Qing Xu Ying Lin Yan Yang Jinzhen Tang Lin Lin Huimin Li Ziquan Lv Zhen Zhang Xuan Zou Xiaolu Shi Shenzhen Center for Disease Control and Prevention Shenzhen 518000China Shenzhen Futian District Center for Disease Control and Prevention Shenzhen 518000China Shenzhen Longgang District Center for Disease Control and Prevention Shenzhen 518000China

This paper comprehensively analyses the first-ever monkeypox outbreak in Shenzhen,China,encompassing clinical symptomatology,therapeutic approaches,epidemiological research,and comprehensive laboratory tests,aiming to establish a robust reference for future monkeypox mitigation and management strategies.The investigation involved a thorough investigation of all identified positive cases,including extensive molecular analysis by nucleic acid detection and whole-genome sequencing of the monkeypox virus.Contact tracing and containment of the infected individuals were also undertaken.Three distinct monkeypox cases were identified in this unique outbreak,exhibiting mild and atypical clinical manifestations,primarily fever and rash.All cases were associated with a single transmission chain,primarily facilitated through close contact and homosexual behavior,indicative of a high-risk factor for monkeypox transmission.

关键词： Monkeypox Epidemiology genetic characteristics

Chromosome stability and genetic characteristics of a novel allohexaploid Brassica developed by selfing of microspore-derived lines

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Chromosome stability and genetic characteristics of a novel ...

Preliminary Analysis of genetic characteristics of Edible Fungus Tricholoma matsutake Isolated from Yajiang, China, by AFLP Technique

中国作物学会油料作物专业委员会第八次会员代表大会暨学术...

作者： ZHANG Kang-ni YANG Su HUANG Qian WANG Jian-su Theodore M.Mwamba ZHOU Wei-jun Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm Zhejiang University

In Brassica,only three diploid species and three allotetraploid species exist in nature.The synthesis of novel allohexaploid Brassica with A,B,C genomes would be a long-term goal in Brassica genetics and play an important role in crop breeding and genetic studies.In this investigation,we self-crossed a doubl haploid(DH)population obtained from microspore culture of an allohexaploid F((HI6-1) from the cross between two recently-synthesized allohexaploid Brassica lines.Plant morphology,chromosome number,plant ploidy,pollen activity,yield and quality traits were studied,tending to select the progenies with stable chromosomes and good characters.In addition,we used single nucleotide polymorphism(SNP) genetic variation based on restriction site-related DNA(RAD) sequencing to construct a high-density genetic linkage map of the population(including parents and146 DH progenies).As a result,a genetic map of this allohexaploid Brassica DH population was constructed with7 950 high quality SNP markers,which segregated normally(1:1) in the population.The linkage map contained all 27 chromosomes from the parental A,B and C genomes with a total genetic distance of 5 725.19 cM and an average of 0.75 cM between adjacent markers.We detected 5 fertility-related traits associated with 25 additive QTLs.Moreover,44 intra-genomic and 18 inter-genomic epistatic QTL pairs were detected for 4 phenotypic traits.Phenotypic,phenological parameters as well as yield components and quality traits were also investigated and compared between different generations.The results provide a foundation for improving stability and fertility in a future allohexaploid Brassica species.

关键词： allohexaploid Brassica chromosome stability genetic characteristics SNP QTL mapping

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Agricultural Sciences in China 2003年第2期2卷 229-236页

作者： CHEN Qiang, LIAO De-cong, ZHANG Xiao-ping, ZHANG Chang-bing and LI Deng-yuDepartment of Microbiology , Sichuan Agricultural University, Ya’an 625000, P.R. China Institute of Sichuan Grassland Science, Chengdu 611731 , P. R. China Department of Microbiology Sichuan Agricultural University Ya'an 625000 P. R. China

A total of 10 Tricholoma matsutake strains isolated from Yajiang County, Sichuan Province of China were analyzed by using AFLP technique. The results showed that the genetic characteristics of Tricholoma matsutake strains can be properly revealed by AFLP, and among the 10 Tricholoma matsutake strains, the genetic aspects were very similar. At the level of 90% similarity, all the strains collected together, and at the similarity of 92 % , three AFLP groups formed. Simultaneously, the effects of quantity of the primers on the second AFLP PCR were determined. The optimal quantity of Eco +1/Mse+1, Eco + 2/Mse + 2 and Eco + 3/ Mse+3 primer pairs used in the AFLP was 1 pmol/5 pmol, 0. 6 pmol/10 pmol and 0.1 pmol/8 pmol, respectively.

关键词： Tricholoma matsutake genetic characteristics AFLP

Comprehensive characterization of the genetic landscape of familial Hirschsprung's disease

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World Journal of Pediatrics 2023年第7期19卷 644-651页

作者： Jun Xiao Lu-Wen Hao Jing Wang Xiao-Si Yu Jing-Yi You Ze-Jian Li Han-Dan Mao Xin-Yao Meng Jie-Xiong Feng Department of Pediatric Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology1095 Jiefang AvenueWuhan 430030China Hubei Clinical Center of Hirschsprung's Disease and Allied Disorders Wuhan 430030China Department of Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology1095 Jiefang AvenueWuhan 430030China

Background Hirschsprung's disease(HSCR)is one of the most common congenital digestive tract malformations and can cause stubborn constipation or gastrointestinal obstruction after birth,causing great physical and mental pain to patients and their families.Studies have shown that more than 20 genes are involved in HSCR,and most cases of HSCR are sporadic.However,the overall rate of familial recurrence in 4331 cases of HSCR is about 7.6%.Furthermore,familial HSCR patients show incomplete dominance.We still do not know the penetrance and genetic characteristics of these known risk genes due to the rarity of HSCR families.Methods To find published references,we used the title/abstract terms"Hirschsprung"and"familial"in the PubMed data-base and the MeSH terms"Hirschsprung"and"familial"in Web of Science.Finally,we summarized 129 HSCR families over the last 40 years.Results The male-to-female ratio and the percentage of short segment-HSCR in familial HSCR are much lower than in sporadic HSCR.The primary gene factors in the syndromic families are ret proto-oncogene(RET)and endothelin B receptor gene(EDNRB).Most families show incomplete dominance and are relevant to RET,and the RET mutation has 56%pen-etrance in familial HSCR.When one of the parents is a RET mutation carrier in an HSCR family,the offspring's recurrence risk is 28%,and the incidence of the offspring does not depend on whether the parent suffers from HSCR.Conclusion Our findings will help HSCR patients obtain better genetic counseling,calculate the risk of recurrence,and provide new insights for future pedigree studies.

关键词： genetic characteristics Hirschsprung's disease Penetrance Recurrence risk Ret proto-oncogene(RET)

Geological characteristics and models of fault-foldfracture body in deep tight sandstone of the second member of Upper Triassic Xujiahe Formation in Xinchang structural belt of Sichuan Basin,SW China

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Petroleum Exploration and Development 2023年第3期50卷 603-614页

作者： LIU Junlong LIU Zhongqun LIU Zhenfeng LIU Yali SHEN Baojian XIAO Kaihua BI Youyi WANG Xiaowen WANG Ail FAN Lingxiao LI Jitongl Sinopec Petroleum Exploration&Production Research Institute Beijing 100083China Sinopec Southwest Oil&Gas Company Chengdu 610081China

In the second member of the Upper Triassic Xujiahe Formation(T_(3)x_(2))in the Xinchang area,western Sichuan Basin,only a low percent of reserves has been recovered,and the geological model of gas reservoir sweet spot remains unclear.Based on a large number of core,field outcrop,test and logging-seismic data,the T_(3)x_(2) gas reservoir in the Xinchang area is examined.The concept of fault-fold-fracture body(FFFB)is proposed,and its types are recognized.The main factors controlling fracture development are identified,and the geological models of FFFB are established.FFFB refers to faults,folds and associated fractures reservoirs.According to the characteristics and genesis,FFFBs can be divided into three types:fault-fracture body,fold-fracture body,and fault-fold body.In the hanging wall of the fault,the closer to the fault,the more developed the effective fractures;the greater the fold amplitude and the closer to the fold hinge plane,the more developed the effective fractures.Two types of geological models of FFFB are established:fault-fold fracture,and matrix storage and permeability.The former can be divided into two subtypes:network fracture,and single structural fracture,and the later can be divided into three subtypes:bedding fracture,low permeability pore,and extremely low permeability pore.The process for evaluating favorable FFFB zones was formed to define favorable development targets and support the well deployment for purpose of high production.The study results provide a reference for the exploration and development of deep tight sandstone oil and gas reservoirs in China.

关键词： fault-fold-fracture body fracture control factor genetic characteristics geological model deep layer tight sandstone Xinchang structural belt Upper Triassic Xujiahe Formation Sichuan Basin

Spread of invasive migratory pest Spodoptera frugiperda and management practices throughout China

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Journal of Integrative Agriculture 2021年第3期20卷 637-645页

作者： ZHOU Yan WU Qiu-lin ZHANG Hao-wen WU Kong-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

The fall armyworm(FAW),Spodoptera frugiperda(J.E.Smith)(Lepidoptera:Noctuidae),a notorious migratory pest native to tropical and subtropical America,invaded China in December 2018,then spread through 26 provinces(autonomous regions,municipalities)in 2019 and 27 in 2020,damaging 1.125 and 1.278 million hectares of crops,respectively.Maize was the most severely affected crop,although wheat and other plants were also ruined.Considering the biological characteristics,incidence regularity and migration patterns of the FAW populations,Chinese government implemented a regional control strategy and divided the areas infested with FAW into the annual breeding grounds in Southwest and South China,the transitional migration area in Jiangnan and Jianghuai and the key preventive area in the Huang-Huai-Hai region and North China.The National Agro-Tech Extension and Service Center constructed"the National Information Platform for the Prevention and Control of the Fall Armyworm"at the county level,which would entail people reporting and mapping the spread of fall armyworm.According to forecasting information,millions of extension workers and small-scale growers in entire country were rallied by local governments to fight the pest through comprehensive control tactics including chemical,physical,biological and ecological measures.Thanks to the joint prevention and control,the final loss of crops infested was controlled within 5%of the total in 2019 and 2020.This review also gives a discussion on existing problems and future management scenarios.

关键词： Spodoptera frugiperda genetic characteristics occurrence and damage monitoring and early-warning control measures

Summary of Short-vine Watermelon Breeding Practice

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Agricultural Biotechnology 2023年第2期12卷 26-27,41页

作者： Kun CHEN Jianfeng SUN Xianfu HOU Shangqiu Academy of Agriculture and Forestry Sciences Shangqiu 476000China Henan Aoxue Agricultural Technology Co. Ltd.Shangqiu 476000China

This paper expounded the current situation and genetic mechanisms of short-vine watermelon breeding from the aspects of material sources,breeding process and genetic characteristics of F_1,hoping to provide a theoretical basis for short-vine watermelon breeding,and breeding materials for watermelon planting innovation,as well as new opportunities for high-quality and high-yield watermelon.

关键词： Watermelon Short vine Breeding genetic characteristics

A multi-center study on Molecular Epidemiology of Human Respiratory Syncytial Virus from Children with Acute Lower Respiratory Tract Infections in the Mainland of China between 2015 and 2019

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Virologica Sinica 2021年第6期36卷 1475-1483页

作者： Xiangpeng Chen Yun Zhu Wei Wang Changchong Li Shuhua An Gen Lu Rong Jin Baoping Xu Yunlian Zhou Aihuan Chen Lei Li Meng Zhang Zhengde Xie Beijing Key Laboratory of Pediatric Respiratory Infection Diseases Key Laboratory of Major Diseases in ChildrenMinistry of EducationNational Clinical Research Center for Respiratory DiseasesResearch Unit of Critical Infection in ChildrenChinese Academy of Medical Sciences2019RU016Laboratory of Infection and VirologyBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China The 2nd Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou 325027China Children's Hospital of Hebei Province Shijiazhuang 050031China Guangzhou Women and Children's Medical Center Guangzhou 510623China Guiyang Maternal and Child Health Hospital Guiyang 550003China Beijing Children's Hospital Capital Medical UniversityNational Center for Children's HealthBeijing 10045China The Children's Hospital of Zhejiang University School of Medicine Hangzhou 310003China The First Affiliated Hospital of Guangzhou Medical University Guangzhou 510120China Yinchuan Maternal and Child Health Care Hospital Yinchuan 750001China

Human respiratory syncytial virus(RSV) is a major pathogen of acute lower respiratory tract infection among young children. To investigate the prevalence and genetic characteristics of RSV in China, we performed a molecular epidemiological study during 2015–2019. A total of 964 RSV-positive specimens were identified from 5529 enrolled patients during a multi-center study. RSV subgroup A(RSV-A) was the predominant subgroup during this research period except in2016. Totally, 535 sequences of the second hypervariable region(HVR-2) of the G gene were obtained. Combined with182 Chinese sequences from GenBank, phylogenetic trees showed that 521 RSV-A sequences fell in genotypes ON1(512),NA1(6) and GA5(3), respectively;while 196 RSV-B sequences fell in BA9(193) and SAB4(3). ON1 and BA9 were the only genotypes after December 2015. Genotypes ON1 and BA9 can be separated into 10 and 7 lineages, respectively. The HVR-2 of genotype ON1 had six amino acid changes with a frequency more than 10%, while two substitutions H258 Q and H266 L were co-occurrences. The HVR-2 of genotype BA9 had nine amino acid substitutions with a frequency more than10%, while the sequences with T290 I and T312 I were all from 2018 to 2019. One N-glycosylation site at 237 was identified among ON1 sequences, while two N-glycosylation sites(296 and 310) were identified in the 60-nucleotide duplication region of BA9. To conclusion, ON1 and BA9 were the predominant genotypes in China during 2015–2019. For the genotypes ON1 and BA9, the G gene exhibited relatively high diversity and evolved continuously.

关键词： Human respiratory syncytial virus(RSV) genetic characteristics Molecular epidemiology Genotype Acute lower respiratory tract infection(ALRTI)

A Review of Fatty Acids and genetic Characterization of Safflower(Carthamus Tinctorius L.) Seed Oil

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World Journal of Traditional Chinese Medicine 2016年第2期2卷 48-52页

作者： Lei Liu Ling-Liang Guan Yu-Xia Yang College of Life Science & Biotechnology Mianyang Normal University Tropical Crops genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences Sichuan Academy of Traditional Chinese Medicine Sciences

Safflower, Carthamus tinctorius L., is an annual oilseed crop that is cultivated on small plots all over the world. The seed oil content ranges from 20% to 45%; the oil is high in linoleic acid, an unsaturated fatty acid that aids in lowering the blood cholesterol level. Thus, safflower has long been used as medical plant in many countries, especially in China and India. However, for industrial purposes, it has long been neglected because of the low seed yield or oil content, until its physical role was revealed. In recent years, research works carried out in many countries mostly focused on improving the seed or oil yield. In this review, after illustrating the fatty acid composition of safflower seed oil as well as the genetic characteristics of safflower and their relationships with agronomic traits, a brief analysis of the current worldwide situation and future prospects of safflower utilization are presented.

关键词： Safflower fatty acid seed development genetic characteristics agronomic traits

Advances in Agaricus bitorquis genetics and Breeding Research

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食用菌学报 2007年第4期 81-86页

作者： CAI Weiming1,JIN Qunli1,FENG Weilin1,FAN Lijun1,LI Nanyi1(Horticultural Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zheijiang 310021,China)

Advances in research on Agaricus bitorquis mating and breeding systems,and the breeding of new strains of this mushroom both in China and overseas,have been reviewed.Progress in our understanding of the genetic basis of homokaryotic fruiting,nuclear migration and mitochondrial inheritance,has been presented.The three main groups of A.bitorquis strains (temperate,bridging and tropical) categorized on the basis of mating behavior,and the distribution of homogenic and heterogenic incompatibility within the groups,have been discussed.Existing problems have been identified,and proposals made for future research.

关键词： Agaricus bitorquis genetic characteristics Breeding advances