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DMPP mitigates N_(2)O and NO productions by inhibiting ammonia-oxidizing bacteria in an intensified vegetable field under different temperature and moisture regimes

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Pedosphere 2024年第3期34卷 652-663页

作者： Xi ZHANG Xintong XU Chenyuan WANG Qianqian ZHANG Yubing DONG Zhengqin XIONG Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjing 210095(China) Department of Soil Science of Temperate Ecosystems Department of Agricultural Soil ScienceGeorg-August University of GottingenGottingen 37077(Germany) State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300(China) Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu Jiangsu Academy of Agricultural SciencesHuai’an 223001(China)

Vegetable soils with high nitrogen input are major sources of nitrous oxide(N_(2)O)and nitric oxide(NO),and incorporation of the nitrification inhibitor 3,4-dimethylpyrazole phosphate(DMPP)into soils has been documented to effectively reduce ***,the efficiency of DMPP in terms of soil N_(2)O and NO mitigations varies greatly depending on soil temperature and moisture ***,further evaluations of DMPP efficiency in diverse environments are required to encourage widespread application.A laboratory incubation study(28 d)was established to investigate the interactive effects of DMPP,temperature(15,25,and 35?C),and soil moisture(55% and 80% of water-holding capacity(WHC))on net nitrification rate,N_(2)O and NO productions,and gene abundances of nitrifiers and denitrifiers in an intensive vegetable *** showed that incubating soil with 1%DMPP led to partial inhibition of the net nitrification rate and N_(2)O and NO productions,and the reduction percentage of N_(2)O production was higher than that of NO production(69.3%vs.38.2%)regardless of temperature and soil moisture *** increased temperatures promoted the net nitrification rate but decreased soil N_(2)O and NO *** moisture influenced NO production more than N_(2)O production,decreasing with the increased moisture level(80%).The inhibitory effect of DMPP on cumulative N_(2)O and NO productions decreased with increased temperatures at 55%***,the inhibitory effect of DMPP on cumulative N_(2)O production increased with increased temperatures at 80%*** on the correlation analyses and automatic linear modeling,the mitigation of both N_(2)O and NO productions from the soil induced by DMPP was attributed to the decreases in ammonia-oxidizing bacteria(AOB)amoA gene abundance and NO_(2)^(-)-N ***,our study indicated that DMPP reduced both N_(2)O and NO productions by regulating the associated AOB amoA gene abundance and NO_(2)^(-)-N concentra

关键词： 3,4-dimethylpyrazole phosphate environment condition gene abundance nitrification inhibitor nitrite accumulation soil water content

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Changes in the activities of key enzymes and the abundance of functional genes involved in nitrogen transformation in rice rhizosphere soil under different aerated conditions

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Journal of Integrative Agriculture 2023年第3期22卷 923-934页

作者： XU Chun-mei XIAO De-shun CHEN Song CHU Guang LIU Yuan-hui ZHANG Xiu-fu WANG Dan-ying The State Key Laboratoty of Rice Biology China National Rice Research InstituteHangzhou 310006P.R.China

Soil microorganisms play important roles in nitrogen transformation. The aim of this study was to characterize changes in the activity of nitrogen transformation enzymes and the abundance of nitrogen function genes in rhizosphere soil aerated using three different methods(continuous flooding(CF), continuous flooding and aeration(CFA), and alternate wetting and drying(AWD)). The abundances of amoA ammonia-oxidizing archaea(AOA) and ammonia-oxidizing bacteria(AOB), nirS, nirK, and nifH genes, and the activities of urease, protease, ammonia oxidase, nitrate reductase, and nitrite reductase were measured at the tillering(S1), heading(S2), and ripening(S3) stages. We analyzed the relationships of the aforementioned microbial activity indices, in addition to soil microbial biomass carbon(MBC) and soil microbial biomass nitrogen(MBN), with the concentration of soil nitrate and ammonium nitrogen. The abundance of nitrogen function genes and the activities of nitrogen invertase in rice rhizosphere soil were higher at S2 compared with S1 and S3 in all treatments. AWD and CFA increased the abundance of amoA and nifH genes, and the activities of urease, protease, and ammonia oxidase, and decreased the abundance of nirS and nirK genes and the activities of nitrate reductase and nitrite reductase, with the effect of AWD being particularly strong. During the entire growth period, the mean abundances of the AOA amoA, AOB amoA, and nifH genes were 2.9, 5.8, and 3.0 higher in the AWD treatment than in the CF treatment, respectively, and the activities of urease, protease, and ammonia oxidase were 1.1, 0.5, and 0.7 higher in the AWD treatment than in the CF treatment, respectively. The abundances of the nirS and nirK genes, and the activities of nitrate reductase and nitrite reductase were 73.6, 84.8, 10.3 and 36.5% lower in the AWD treatment than in the CF treatment, respectively. The abundances of the AOA amoA, AOB amoA, and nifH genes were significantly and positively correlated wi

关键词： rhizosphere aeration gene abundance enzyme activities soil microbial biomass carbon soil microbial nitrogen

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Decreased nitrous oxide emissions associated with functional microbial genes under bio-organic fertilizer application in vegetable fields

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Pedosphere 2021年第2期31卷 279-288页

作者： Yajun GENG Yiming YUAN Yingcheng MIAO Junzhang ZHI Mengyuan HUANG Yihe ZHANG Hong WANG Qirong SHEN Jianwen ZOU Shuqing LI Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjing 210095(China) Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource UtilizationNanjing Agricultural UniversityNanjing 210095(China) College of Resource and Environment Anhui Science and Technology UniversityChuzhou 233100(China)

Bio-organic fertilizers enriched with plant growth-promoting microbes(PGPMs)have been widely used in crop fields to promote plant growth and maintain soil microbiome ***,their potential effects on N_(2)O emissions are of increasing *** this study,an in situ measurement experiment was conducted to investigate the effect of organic fertilizer containing Trichoderma guizhouense(a plant growth-promoting fungus)on soil N_(2)O emissions from a greenhouse vegetable *** following four treatments were used:no fertilizer(control),chemical fertilizer(NPK),organic fertilizer derived from cattle manure(O),and organic fertilizer containing ***(O+T,referring to bio-organic fertilizer).The abundances of soil N cycling-related functional genes(amoA)from ammonium-oxidizing bacteria(AOB)and archaea(AOA),as well as nirS,nirK,and nosZ,were simultaneously determined using quantitative PCR(qPCR).Compared to the NPK plot,seasonal total N_(2)O emissions decreased by 11.7%and 18.7%in the O and O+T plots,respectively,which was attributed to lower NH_(4)^(+)-N content and AOB amoA abundance in the O and O+T *** nosZ abundance was significantly greater in the O+T plot,whilst the AOB amoA abundance was significantly lower in the O+T plot than in the O *** to the organic fertilizer,bio-organic fertilizer application tended to decrease N_(2)O emissions by 7.9%and enhanced vegetable yield,resulting in a significant decrease in yield-scaled N_(2)O ***,the results of this study suggested that,compared to organic and chemical fertilizers,bio-organic fertilizers containing PGPMs could benefit crop yield and mitigate N_(2)O emissions in vegetable fields.

关键词： chemical fertilizer gene abundance greenhouse vegetable N cycle-related genes plant growth-promoting microbe Trichoderma guizhouense yield-scaled N_(2)O emission

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Integrated management of crop residue and nutrient enhances new carbon formation by regulating microbial taxa and enzymes

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Journal of Integrative Agriculture 2022年第6期21卷 1772-1785页

作者： WU Hong-liang CAI An-dong XING Ting-ting HUAI Sheng-chang ZHU Ping HAN Xiao-zeng XU Ming-gang LU Chang-ai Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable LandBeijing 100081P.R.China Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Agricultural Resources and Environment Jilin Academy of Agricultural SciencesChangchun 130033P.R.China Northeast Institute of Geography and Agroecology Chinese Academy of SciencesChangchun 130102P.R.China College of Resources and Environment Shanxi Agricultural UniversityTaiyuan 030031P.R.China

Although returning crop residue to fields is a recommended measure for improving soil carbon(C)stocks in agroecosystems,the response of newly formed soil C(NFC)to the integrated supply of residue and nutrients and the microbial mechanisms involved in NFC are not fully ***,an 84-day incubation experiment was conducted to ascertain the microbial mechanisms that underpin the NFC response to inputs of residue and nitrogen(N),phosphorus(P),and sulfur(S)in two black(Phaeozem)soils from experimental plots at Gongzhuling,Jilin Province and Hailun,Heilongjiang Province,*** results showed that adding residue alone accelerated microbial nutrient mining,which was supported by decreases of 8^(-1)6%in the ratios of C:N and C:P enzyme activities,relative to soils with nutrient *** NFC amounts increased from 1156 to 1722 mg kg^(−1) in Gongzhuling soil and from 725 to 1067 mg kg^(−1) in Hailun soil as the levels of nutrient supplementation *** regression tree analysis suggested thatβ-glucosidase(BG),acid phosphatase(AP),microbial biomass C(MBC),and Acidobacteria accounted for 27.8,18.5,14.7,and 8.1%,respectively,of the NFC in Gongzhuling soil and for 25.9,29.5,10.1,and 13.9%,respectively,of the NFC in Hailun *** analysis determined that Acidobacteria positively influenced NFC both directly and indirectly by regulating BG,AP,and MBC,in which MBC acquisition was regulated more by *** amount of NFC was lower in Hailun soil than in Gongzhuling soil and was directly affected by AP,indicating the importance of soil properties such as SOC and pH in determining ***,our results reveal the response of NFC to supplementation by N,P,and S,which depends on Acidobacteria and Proteobacteria,and their investment in BG and AP in residue-amended soil.

关键词： newly formed soil carbon extracellular enzyme activities gene abundance nutrient supplementation black soil

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Effects of Inoculum Density on Plant Growth and Hydrocarbon Degradation

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Pedosphere 2016年第5期26卷 774-778页

作者： Ghulam SHABIR Muhammad ARSLAN Kaneez FATIMA Imran AMIN Qaiser Mahmood KHAN Muhammad AFZAL Environmental Biotechnology Division National Institute for Biotechnology and Genetic Engineering (NIBGE) Earth Sciences Department King Fahd University of Petroleum and Minerals

The combined use of plants and bacteria is a promising approach for the remediation of soil contaminated with organic pollutants. Different biotic and abiotie factors can affect the survival and activity of the applied bacteria and consequently plant growth and phy- toremediation efficiency. The effect of inoculum density on the abundance and expression of alkune-degrading genes in the rhizosphere of plant vegetated in hydrocarbon-contaminated soil has been rarely observed. In this study, an alkane-degrading bacterium （Pantoea sp. strain BTRH79）, at different inoculum densities （10^5 to 10^8 cells cm^-3 soil）, was inoculated to ryegrass （Lolium perenne） vegetated in diesel-contaminated soil to find the optimum inoculum density needed for its efficient colonization and hydrocarbon degradation activity. Bacterial inoculation improved plant growth and hydrocarbon degradation. Maximum plant growth and hydrocarbon degra- dation were observed with the inoculum having the highest cell density （10^8 cells cm^-3 soil）. Moreover, the inoculum with higher cell density exhibited more abundance and expression of alkane hydroxylase gene, CYP153. This study suggests that the inoculum density is one of the main factors that can affect bacterial colonization and activity during phytoremediation.

关键词： alkane-degrading bacterium gene abundance gene expression phytoremediation plant-bacteria partnership

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