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gecko crude peptides inhibit migration and lymphangiogenesis by down regulating the expression of VEGF-C in human hepatocellular carcinoma cells and human lymphatic endothelial cells

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中国药理学与毒理学杂志 2017年第10期31卷 958-959页

作者： Meng-li GUO Cai-e WANG Yi-meng DUAN Jian-gang WANG Medical College Henan University of Science and Technology The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology

OBJECTIVE To explore the role of gecko crude peptides(GCPs)in the proliferation,apoptosis,migration and lymphangiogenesis of human hepatocellular carcinoma cells(Hep G2)and human lymphaticendothelial cells(HLECs)in *** The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay was used to evaluate the anti-proliferative effect of GCPs and si RNA-VEGF-C on Hep G2 cells,Hoechst 33258 staining and flow cytometry were performed to analyze cycle and *** migration and invasion ability of cells were assayed by transwell chamber experiment and wound-healing *** protein and m RNA expressions of vascular endothelial growth factor-C(VEGF-C)and CXC chemokine receptor-4(CXCR4)were detected by q-PCR,immunofluorescence,Western *** protein expressions of the extracellular signal regulated kinase(ERKI/2),c-Jun N-terminal kinase(JNK),p38-mitogen activated protein kinases(p38 MAPK),serine/threonine kinase(Akt)and phosphatidylinositol-3-kinase(PI3K)were detected by western *** anti-lymphangiogenesis effect of GCPs on the HLECs was analyzed using an in vitro tube-formation *** protein and m RNA expressions of vascular endothelial growth factor receptor-3(VEGFR-3)and stromal cell-derived factor-1(SDF-1)were detected by q-PCR,Western *** GCPs and si RNA-VEGF-C inhibited Hep G2 proliferation,invasion and migration,and the most obvious inhibitory effect was both synergistic ***,GCPs suppressed HLECs proliferation,migration and tubelike structure formationin a dose-dependent manner,and had inhibitory effect of tumor-induced lymphangiogenesis in ***,we found that GCPs and si RNA-VEGF-C decreased the expressions of MMP-2,MMP-9,VEGF-C,CXCR4,phospho-ERK1/2,phospho-P38,phospho-JNK and PI3K in Hep G2 ***,GCPs had a dose-dependent depressive effecton the expressions of VEGFR-3,SDF-1 in *** The low expression of VEGF-C mediated by si RNA-VEGF-C and GCPs inhibit tumor proliferation,invas

关键词： gecko crude peptides hepatic carcinoma vascular endothelial growth factor-C RNA interference(RNAi) lymphangiogenesis

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