检索结果-南通市图书馆

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

大白鼠视网膜中向同侧中枢投射的神经节细胞

Current Zoology 1985年第2期31卷 117-124页

作者：刁云程石健王永凯卜明亮中国科学院生物物理研究所

在大白鼠视束和上丘注射HRP作逆行标记表明,向对侧中枢投射的神经节细胞遍布视网膜各处,而同侧投射的细胞大部分位于颞下侧边缘的新月形区域内,少数细胞分布在此区以外。与视束注射比较,上丘注射时同侧视网膜颞新月区内标记细胞数大为... 详细信息

在大白鼠视束和上丘注射HRP作逆行标记表明,向对侧中枢投射的神经节细胞遍布视网膜各处,而同侧投射的细胞大部分位于颞下侧边缘的新月形区域内,少数细胞分布在此区以外。与视束注射比较,上丘注射时同侧视网膜颞新月区内标记细胞数大为减少。统计测量表明,同侧投射细胞胞体直径平均比对侧投射细胞的略长。文中对上述结果的生理学意义进行了讨论。

关键词： HEP labeling Eetina ganglion cell Albino rat

Melatonin potentiates glycine currents through distinct PC-PLC/PKC signaling pathway in rat retinal ganglion cells

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Melatonin potentiates glycine currents through distinct PC-P...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Wen-Jie ZHAO,Min ZHANG,Xiong-Li YANG*,Zhong-Feng WANG~* Institute of Neurobiology,Institutes of Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032,China

In vertebrate retina,melatonin regulates various physiological *** previous work demonstrated potentiation of glycine currents by melatonin in rat retinal ganglion cells(RGCs) via activating melatonin MT2 receptors. In the present work we investigated the mechanisms underlying the melatonin effects by whole-cell patch-clamp technique. Immunofluorescence double labeling showed that rat RGCs were solely immunoreactive to MT2 *** potentiated glycine currents of rat RGCs and promoted their desensitization,which were reversed by 4-P-PDOT,a specific antagonist of MT2 *** effects of melatonin were blocked by intracellular dialysis of GDP-β-S,a nonhydrolyzable inhibitor of G-proteins,and antibodies against regulator of G-protein signaling(RGS) 2 and 4 *** addition, preincubation with pertussis toxin(PTX) eliminated the melatonin-induced potentiation of glycine *** these results suggest that the melatonin effects were mediated by PTX-sensitive Gi/o ***,D609,a phosphatidylcholine (PC)-specific phospholipase C(PLC) inhibitor,but not U73122,a phosphatidylinositol(PI)-PLC inhibitor, blocked the melatonin *** protein kinase C(PKC) activator PMA potentiated the glycine currents,thus mimicking the effects of melatonin,and the presence of PMA melatonin no longer caused further potentiation of the currents, whereas application of the PKC inhibitor bisindolylmaleimideⅣ(BisⅣ) abolished the melatonin *** melatonininduced potentiation of glycine currents was calcium-independent,as evidenced by the fact that the effects persisted when intracellular Ca was chelated by *** shown by calcium imaging,melatonin induced no increase in intracellular *** cAMP-PKA nor cGMP-PKG signaling pathways seemed to be involved because application of 8-BrcAMP or 8-Br-cGMP failed to mimic the melatonin *** both the PKA inhibitor H-89 and the PKG inhibitor KT5823 did not block the melatonin-induced potent

关键词： melatonin MT2 receptor glycine receptor Gi/o PC-PLC/PKC pathway RGS2/4 ganglion cell retina

Overexpressing NeuroD1 reprograms Müller cells into various types of retinal neurons

在线全文

cnki会议

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第5期18卷 1124-1131页

作者： Di Xu Li-Ting Zhong Hai-Yang Cheng Zeng-Qiang Wang Xiong-Min Chen Ai-Ying Feng Wei-Yi Chen Gong Chen Ying Xu Guangdong-Hongkong-Macao Institute of CNS Regeneration Key Laboratory of CNS Regeneration(Ministry of Education)Jinan UniversityGuangzhouGuangdong ProvinceChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

The onset of retinal degenerative disease is often associated with neuronal loss. Therefore, how to regenerate new neurons to restore vision is an important issue. NeuroD1 is a neural transcription factor with the ability to reprogram brain astrocytes into neurons in vivo. Here, we demonstrate that in adult mice, NeuroD1 can reprogram Müller cells, the principal glial cell type in the retina, to become retinal neurons. Most strikingly, ectopic expression of NeuroD1 using two different viral vectors converted Müller cells into different cell types. Specifically, AAV7 m8 GFAP681::GFP-ND1 converted Müller cells into inner retinal neurons, including amacrine cells and ganglion cells. In contrast, AAV9 GFAP104::ND1-GFP converted Müller cells into outer retinal neurons such as photoreceptors and horizontal cells, with higher conversion efficiency. Furthermore, we demonstrate that Müller cell conversion induced by AAV9 GFAP104::ND1-GFP displayed clear dose-and time-dependence. These results indicate that Müller cells in adult mice are highly plastic and can be reprogrammed into various subtypes of retinal neurons.

关键词： amacrine cell ganglion cell horizontal cell in vivo reprogramming Müller cell NeuroD1 photoreceptor regeneration retina retinal degeneration

Numerical simulation of neuronal spike patterns in a retinal network model

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2011年第16期6卷 1254-1260页

作者： Lei Wang Shenquan Liu Shanxing Ou Department of Mathematics South China University of Technology Guangzhou 510640 Guangdong Province China Department of Radiology General Hospital of Guangzhou Mifitary Command Guangzhou 510640 Guangdong Province China

This study utilized a neuronal compartment model and NEURON software to study the effects of external light stimulation on retinal photoreceptors and spike patterns of neurons in a retinal network Following light stimulation of different shapes and sizes, changes in the spike features of ganglion cells indicated that different shapes of light stimulation elicited different retinal responses. By manipulating the shape of light stimulation, we investigated the effects of the large number of electrical synapses existing between retinal neurons. Model simulation and analysis suggested that interplexiform cells play an important role in visual signal information processing in the retina, and the findings indicated that our constructed retinal network model was reliable and feasible. In addition, the simulation results demonstrated that ganglion cells exhibited a variety of spike patterns under different light stimulation sizes and different stimulation shapes, which reflect the functions of the retina in signal transmission and processing.

关键词： computational network model retina light stimulation ganglion cell spike pattern

Hyperactivity of ON-type retinal ganglion cells in streptozotocin-induced diabetic mice

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hyperactivity of ON-type retinal ganglion cells in streptozo...

中国神经科学学会第十届全国学术会议

作者： Jun YU Lu WANG Shi-Jun WENG Xiong-Li Yang Dao-Qi ZHANG Yong-Mei ZHONG Institute of Neurobiology Institutes of Brain Science and State Key Laboratory of Medical NeurobiologyFudan University

Objective Impairment of visual function has been detected at the early stage of diabetes,but the underlying neural mechanisms involved are largely *** and functional alterations of retinal ganghon cells,the final output neurons of the vertebrate retina,are thought to be the major cause of visual defects in diabetes but direct evidence to support this notion is *** this study we investigated functional changes of retinal ganglion cells（GCs）in a type 1-like diabetic mouse *** Diabetes was induced with three consecutive injections of streptozotozin in C57BL/6 *** were studied over the course of 3- to 4-months after onset of *** spontaneous spiking activity of retinal GCs was analyzed using loose patch recording techniques,whereas apoptosis of retinal neurons was evaluated in flat-mount retinas by TUNEL *** The spontaneous spiking and bursting activity of retinal GCs were significantly increased in 3- to 4-month-old diabetic retinas and these increases were only observed in ON-type retinal GCs,but not in OFF-type or ON-OFF-type *** this stage of diabetes,no apoptotic signals were detected in the ganglion cell layer of the retina,suggesting that the functional alterations in GCs occur prior to massive GC ***,we found that the increased activity of ON-type GCs was mainly a result of reduced inhibitory signaling to the cells in *** The present study provides the first-ever characterization of retinal GC electrical activity in a type 1 diabetic mouse *** found that ON-type GCs were hyperactive in diabetes and that the hyperactivity appeared to be predominantly mediated by a diabetes-induced reduction in inhibitory amacrine cell *** novel mechanism provides insight into how visual function is impaired in diabetic retinopathy.

关键词： diabetes retina ganglion cell spontaneous activity inhibitory neurotransmitter apoptosis

Changes in morphological and physiological properties of retinal ganglion cells in STZ-induced diabetic mice

在线全文

cnki会议

学校读者我要写书评

暂无评论

Changes in morphological and physiological properties of ret...

中国神经科学学会第十二届全国学术会议

作者： Run-Ze CUI Lu WANG Sheng-Nan QIAO Shi-Jun WENG Xiong-Li YANG Yong-Mei ZHONG Institutes of Brain Science Fudan University

Objective Diabetic retinopathy is a leading cause of blindness in diabetic patients. While loss of retinal ganglion cells（RGCs） has been reported in both diabetic patients and animal models of diabetes, little is known about morphological and functional alterations of RGCs in diabetic mice. Here we investigate changes in morphological and electrophysiological properties of RGCs in a diabetic mouse model. Methods Hyperglycemia was induced by intraperitoneal injection of streptozocin（STZ） and experiments were conducted in flat-mount retinas of 3-month STZ-diabetic mice and age-matched normal mice（control）. Morphology of four subtypes of RGCs was examined in Thy1-YFP transgenic mice, in which RGCs were labeled with yellow fluorescent protein. Using whole-cell patch-clamp recording, electrophysiological properties of two subtypes of RGCs were analyzed in C57 BL/6 mice. Results（1） Comparisons of the genetically identified RGCs in control and diabetic Thy1-YFP mice revealed that some subtypes underwent significant morphological changes 3 months after the onset of diabetes. There was an increase in the number of dendritic branches of both ON-RGA2 and OFF-RGA2 cells, whereas there was a decrease in dendritic field area in ON-RGA2 cells. In contrast, RGC1 cells were structurally unaffected in diabetic mice. RGD cells are bistratified cells and their dendritic arbors distributed in ON sublamina of the inner plexiform layer（IPL） exhibited significant changes, but those distributed in the OFF sublamina of the IPL did not alter their structure in diabetic mice.（2） In 3-month STZ-diabetic mice, ON-RGA2 cells showed a decrease in maximum firing rate, but an increase in half width of action potential. Moreover, resting potential, membrane time constant and maximum firing rate of transient-OFF-RGA2 cells were increased, but half width of action potential was decreased. Conclusion ON-, OFF-RGA2 and RGD cells undergo significant changes in dendritic structure at the early stage of dia

关键词： diabetic retinopathy ganglion cell dendrite electrophysiological properties

Rectal biopsy for Hirschsprung's disease:a review of techniques,pathology,and complications

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2016年第2期12卷 135-141页

作者： Eleanor Dorothy Muise Robert Anthony Cowles Section of Pediatric Surgery Department of SurgeryYale University School of MedicineNew HavenCTUSA

Background:Hirschsprung's disease(HD)is one of the most common congenital anomalies of colorectal function,affecting approximately 1 in 5000 live births,with a 4:1 male *** is characterized by aganglionosis that is most often limited to the rectosigmoid,but can extend proximally along the colon and,in rare instances,reach into the small intestine.A clinical history of delayed passage of meconium beyond 48 hours after birth,physical exam findings of abdominal distention and vomiting,and a contrast enema demonstrating a transition zone are highly suggestive of *** sources:We searched databases including PubMed,Google Scholar,and Scopus for the following key words:Hirschsprung's disease,rectal biopsy,pathology,ganglion cell,nerve trunk hypertrophy,pediatric constipation,and selected publications written in English that were relevant to the scope of this ***:Based on the data presented in the literature,we reviewed 1)biopsy techniques for the diagnosis of Hirschsprung's disease,addressed inadequate biopsies,and complications from rectal biopsy,and 2)pathologic and histologic interpretation of biopsy specimens for the diagnosis of Hirschsprung's ***:A well-executed rectal biopsy with expert pathologic evaluation of the specimen remains the gold standard for the diagnosis of Hirschsprung's disease and is the subject of this review.

关键词： ganglion cell Hirschsprung's disease nerve trunk hypertrophy pediatric constipation rectal biopsy

Localization of dopamine Dl receptor in carp retina

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Localization of dopamine Dl receptor in carp retina

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Xiao-Min ZHANG,Yong-Mei ZHONG,Xiong-Li YANG Institute of Neurobiology,Institutes of Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032,China

＜正＞Dopamine,as an important neuromodulator in the vertebrate retina,exerts its actions through two classes of receptors,known as the D1 and D2 *** this work,localization of D1 receptors in carp retina was studied using immunofluorescence double labeling and confocal laser scanning microscopy.D1-immunoreactivity（-IR） was observed in a lot of cells in the inner nuclear layer（INL） and some cells in the ganglion cell layer（GCL）.Anti-protein kinase C（PKC）, tyrosine hydroxylase（TH） and choline acetyltransferase（ChAT） antibodies were used to label rod-ON bipolar cells, dopaminergic amacrine cells（ACs） and cholinergic ACs,*** labeling experiments showed that D1 was strongly expressed in the somata of rod-ON bipolar cells,whereas the dendrites in the outer plexiform layer（OPL） and the terminals in the inner plexiform（IPL） of these cells were weakly ***-labeled dopaminergic ACs were located in the inner part of the INL and sent processes mainly into the outer sublayer of the IPL.D1 -IR was clearly observed in the somata but not seen in the processes of dopaminergic *** addition,D1 was also expressed in the somata of two types of cholinergic ACs（ACh-a,ACh-b）,with their cell bodies located in INL and GCL,respectively,but not in their processes in the ***,labeling for D1 was also observed in the ganglion cells which were retrogradely labelled by fluorogold. The wide expression profile of D1 suggests that the retinal dopaminergic system is organized in a way that favors its working through both wiring and volume transmission.

关键词： dopamine D1 receptor immunofluorescence double labeling bipolar cell amacrine cell ganglion cell