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Comparative proteomics analysis of tea leaves exposed to subzero temperature:Molecular mechanism of freeze injury

International Journal of Agricultural and Biological Engineering 2013年第4期6卷 27-34页

作者： Hu Yongguang Lu Yongzong Lu Jian Institute of Agricultural Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Institute of Life Sciences Jiangsu UniversityZhenjiang 212013JiangsuChina

Tea freeze injury is one of the most severe agro-meteorological disasters,which leads to sizable losses of tea production in *** freezing resistant ability of overwintering tea trees becomes weaker and weaker from early-spring to *** it decreases to critical temperature of-2℃or lower in the stage with one or two leaves,tea trees suffer from freeze injury and the yield or quality of spring tea production could decrease *** measurements have been taken to prevent such damage,the physiological and biochemical mechanism of how tea(Camellia Sinensis)plant response to freeze injury is still to be elucidated.A comparative proteomics analysis was made on tea leaves at the two-leaf *** differential image analysis showed 46 spots with density changes(29 spots increased and 17 spots decreased;pfreeze injury group compared with the control *** eight differential protein spots(pfreeze *** results would greatly increase the comprehension of the molecular mechanism for freeze injury and provide a better decision making for freeze protection and control.

关键词： freeze injury molecular mechanism freeze protection comparative proteomics Camellia Sinensis mass spectrometry

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Monitoring Winter Wheat freeze injury Using Multi-Temporal MODIS Data

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Agricultural Sciences in China 2009年第9期8卷 1053-1062页

作者： FENG Mei-chen YANG Wu-de CAO Liang-liang DING Guang-wei College of Agriculture Shanxi Agricultural University Taigu 030801 P.R. China Department of Chemistry Northern State University Aberdeen SD 57401 USA

freeze injury is an usual disaster for winter wheat in Shanxi Province, China, and monitoring freeze injury is of important economic significance. The aim of this article is to monitor and analyze the winter wheat freeze injury using remote sensing data, to monitor the occurrence and spatial distribution of winter wheat freeze in time, as well as the severity of the damage. The winter wheat freeze injury was monitored using multi-temporal moderate-resolution imaging spectroradiometer （MODIS） data, combined with ground meteorological data and field survey data, the change of normalized difference vegetation index （NDVI） before and after freeze injury was analyzed, as well as the effect of winter wheat growth recovery rate on yield. The results showed that the NDVI of winter wheat decreased dramatically after the suffering from freeze injury, which was the prominent feature for the winter wheat freeze injury monitoring. The degrees of winter wheat freeze injury were different in the three regions, of which, Yuncheng was the worst severity and the largest freeze injury area, the severity of freeze injury correlates with the breeding stage of the winter wheat. The yield of winter wheat showed positive correlation with its growth recovery rate （r=0.659^＊＊ which can be utilized to monitor the severity of winter wheat freeze injury as well as its impact on yield. It can effectively monitor the occurrence and severity of winter wheat freeze injury using horizontal and vertical profile distribution and growth wheat freeze injury in Shanxi Province. recovery rate, and provide a basis for monitoring the winter

关键词： winter wheat TM MODIS freeze injury growth recovery rate monitoring

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Assessing winter oilseed rape freeze injury based on Chinese HJ remote sensing data

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第2期16卷 131-144页

作者： Bao SHE Jing-feng HUANG Rui-fang GUO Hong-bin WANG Jing WANG Institute of Agricultural Remote Sensing and Information Technology Application College of Environmental and Resource SciencesZhejiang University Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Feidong Agricultural Technology Extension Center

The winter oilseed rape（Brassica napus L.） accounts for about 90% of the total acreage of oilseed rape in China. However, it suffers the risk of freeze injury during the winter. In this study, we used Chinese HJ-1A/1B CCD sensors, which have a revisit frequency of 2 d as well as 30 m spatial resolution, to monitor the freeze injury of oilseed rape. Mahalanobis distance-derived growing regions in a normal year were taken as the benchmark, and a mask method was applied to obtain the growing regions in the 2010–2011 growing season. The normalized difference vegetation index（NDVI） was chosen as the indicator of the degree of damage. The amount of crop damage was determined from the difference in the NDVI before and after the freeze. There was spatial variability in the amount of crop damage, so we examined three factors that may affect the degree of freeze injury： terrain, soil moisture, and crop growth before the freeze. The results showed that all these factors were significantly correlated with freeze injury degree（P0.01, two-tailed）. The damage was generally more serious in low-lying and drought-prone areas; in addition, oilseed rape planted on south- and west-oriented facing slopes and those with luxuriant growth status tended to be more susceptible to freeze injury. Furthermore, land surface temperature（LST） of the coldest day, soil moisture, pre-freeze growth and altitude were in descending order of importance in determining the degree of damage. The findings proposed in this paper would be helpful in understanding the occurrence and severity distribution of oilseed rape freeze injury under certain natural or vegetation conditions, and thus help in mitigation of this kind of meteorological disaster in southern China.

关键词： Brassica napus freeze injury Remote sensing Crop monitoring HJ-CCD

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Effects of Facilities Condition on Occurrence of freeze injury and Fresh Pod Yield of Broad Bean

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Agricultural Science & Technology 2015年第5期16卷 890-897页

作者：吴春芳卞晓春曹云英夏礼如江苏沿江地区农业科学研究所江苏如皋226541

Using Tongxian No.2 as material, the effects of different film-covering time, different sowing time and different planting density on the occurrence of freeze injury and yield of fresh broad beans were investigated. The randomized block design was adopted. The results showed that with the delayed film covering, the incidence of mild freeze injury and number of headless seedlings were increased correspondingly, but the yield was increased; with the delayed sowing, the branch number per plant, effective branch number per plant, incidence of mild freeze injury and number of headless seedlings were all reduced, and the broad beans, sowed on September 30 th, obtained the highest yield; planting density showed on effect on the occurrence of freeze injury, and the yield was increased with the increase of planting density. Under the same film-covering time, the incidence of freeze injury was reduced with the delayed sowing time and it showed no changes when planting density was changed, but the yield was increased with the increase of planting density and it was highest when broad bean seeds were sowed on September 30th;under the same sowing time, the incidence of freeze injury was increased with the delayed film-covering time and it showed no changes when planting density was changed, and the yield was increased with the delayed film-covering time and increased planting density; under the same planting density, the incidence of freeze injury was increased with the delayed film-covering time but was reduced with the delayed sowing time, and the yield was increased with the delayed film-covering time and it was highest when the broad bean seeds were sowed on September30 th. Under same film-covering time and sowing time, the total branch number per plant and effective branch number per plant were reduced, but the yield was increased with the increase of planting density; under same film-covering time and planting density, the incidence of freeze injury was reduced with the de

关键词： Facility broad bean Film-covering time Sowing time Planting density freeze injury Fresh pod yield

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Tea leaf’s microstructure and ultrastructure response to low temperature in indicating critical damage temperature

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Information Processing in Agriculture 2019年第2期6卷 247-254页

作者： Yongzong Lu Yongguang Hu Richard LSnyder Eric RKent Key Laboratory of Modern Agricultural Equipment and Technology Ministry of Education Jiangsu ProvinceJiangsu UniversityZhenjiangJiangsu 212013China Department of Land Air and Water ResourcesUniversity of CaliforniaDavisDavis 95616USA Department of Plant Sciences University of CaliforniaDavisDavis 95616USA

To find out the critical damage temperature of tea leaf,a new method of subzero treatment was provided by fitting the air temperature data from six heavy frost ***,the study explored the characteristics of Fuding Dabai tea plant response to low temperature stress of 2,0,2,4,8,10 and15C by observing the microstructure and ultrastructure changes of the *** samples were collected in an ambient temperature of 8.6C which served as *** electron microscopy(SEM)and transmission electron microscopy(TEM)were used to observe the microstructure and ultrastructure of stomata and *** observation results indicated that stomata of tea leaves have an obvious low temperature stress when the temperature was lower than2*** extent of opening of the stomata increased and enhanced guard cell protection of tea leaves against cold ***,dehydration,shrinkage and deformation of cells occurred as the temperature decreased from2C to15*** observations showed that the cell nucleus,cell walls,chloroplasts and mitochondria all had normal structure at a temperature of 8.6C where the membrane and granum lamella were clearly *** the temperature decreased to2C,the membrane system of tea leaf was the first to be damaged and the cell walls became ***,the leaf microstructure and ultrastructure showed obvious changes at2C,which might define the critical damage temperature for freeze damage of Fuding Dabai *** strategy based this critical damage temperature is useful for wind machine frost protection in tea fields within the Yangtze River region.

关键词： Frost damage freeze injury Chill injury Frost protection

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