Tea leaf’s microstructure and ultrastructure response to low temperature in indicating critical damage temperature

作     者：Yongzong Lu Yongguang Hu Richard LSnyder Eric RKent 

作者机构：Key Laboratory of Modern Agricultural Equipment and TechnologyMinistry of Education Jiangsu ProvinceJiangsu UniversityZhenjiangJiangsu 212013China Department of LandAir and Water ResourcesUniversity of CaliforniaDavisDavis 95616USA Department of Plant SciencesUniversity of CaliforniaDavisDavis 95616USA 

出 版 物：《Information Processing in Agriculture》 (农业信息处理（英文）)

年 卷 期：2019年第6卷第2期

页      面：247-254页

核心收录：

学科分类：0907[农学-林学] 0908[农学-水产] 08[工学] 0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0905[农学-畜牧学] 0707[理学-海洋科学] 0906[农学-兽医学] 0829[工学-林业工程] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0901[农学-作物学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：The authors are grateful to the financial support by Key R&D Program of Jiangsu Province(BE2016354) Jiangsu Agriculture Science and Technology Innovation Fund(CX(16)1045) Priority Academic Program Development of Jiangsu Higher Education Institutions(2014-37) Project of Innovation of Postgraduate of Jiangsu Province(KYCX17_1788) China Scholarship Council 

主　　题：Frost damage Freeze injury Chill injury Frost protection 

摘      要：To find out the critical damage temperature of tea leaf,a new method of subzero treatment was provided by fitting the air temperature data from six heavy frost ***,the study explored the characteristics of Fuding Dabai tea plant response to low temperature stress of 2,0,
2,
4,
8,
10 and
15
C by observing the microstructure and ultrastructure changes of the *** samples were collected in an ambient temperature of 8.6
C which served as *** electron microscopy(SEM)and transmission electron microscopy(TEM)were used to observe the microstructure and ultrastructure of stomata and *** observation results indicated that stomata of tea leaves have an obvious low temperature stress when the temperature was lower than
2
*** extent of opening of the stomata increased and enhanced guard cell protection of tea leaves against cold ***,dehydration,shrinkage and deformation of cells occurred as the temperature decreased from
2
C to
15
*** observations showed that the cell nucleus,cell walls,chloroplasts and mitochondria all had normal structure at a temperature of 8.6
C where the membrane and granum lamella were clearly *** the temperature decreased to
2
C,the membrane system of tea leaf was the first to be damaged and the cell walls became ***,the leaf microstructure and ultrastructure showed obvious changes at
2
C,which might define the critical damage temperature for freeze damage of Fuding Dabai *** strategy based this critical damage temperature is useful for wind machine frost protection in tea fields within the Yangtze River region.