检索结果-南通市图书馆

mIgM-mediated splenic marginal zone B cells targeting of folic acid for immunological evasion

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第2期14卷 808-820页

作者： Huan Wang Zhuxuan Jiang Zhiwei Guo Gan Luo Tianhao Ding Changyou Zhan Department of Pharmacology School of Basic Medical Sciences&Department of PharmacyShanghai Pudong HospitalFudan UniversityShanghai 200032China Department of Pharmaceutical Sciences School of PharmacyNaval Medical UniversityShanghai 200433China Shanghai Engineering Research Center for Synthetic Immunology Fudan UniversityShanghai 200032China

folic acid is a fully oxidized synthetic folate with high bioavailability and stability which has been extensively prescribed to prevent congenital disabilities.Here we revealed the immunosuppressive effect of folic acid by targeting splenic marginal zone B(MZB)cells.folic acid demonstrates avid binding with the Fc domain of immunoglobulin M(IgM),targeting IgM positive MZB cells in vivo to destabilize IgM-B cell receptor(BCR)complex and block immune responses.The induced anergy of MZB cells by folic acid provides an immunological escaping window for antigens.Covalent conjugation of folic acid with therapeutic proteins and antibodies induces immunological evasion to mitigate the production of anti-drug antibodies,which is a major obstacle to the long-term treatment of biologics by reducing curative effects and/or causing adverse reactions.folic acid acts as a safe and effective immunosuppressant via IgM-mediated MZB cells targeting to boost the clinical outcomes of biologics by inhibiting the production of anti-drug antibodies,and also holds the potential to treat other indications that adverse immune responses need to be transiently shut off.

关键词： Membrane-bound IgM Anti-drugantibodies Bcell anergy folic acid Marginal zoneBcells Bcell receptor Biologics Targeting

A folic acid-decorated nanoparticles loaded JQ1 for oral squamous cell carcinoma therapy

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第3期35卷 332-336页

作者： Dequan Zeng Zhongkai Ma Xin Zan Ting Luo Xiang Wang Xiang Gao Xianghui Fu School of Health Management Xihua UniversityChengdu 610039China State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China College of StomatologySichuan UniversityChengdu 610041China Department of Neurosurgery and Institute of Neurosurgery State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalWest China Medical SchoolSichuan University and Collaborative Innovation Center for BiotherapyChengdu 610041China Breast Disease Center Cancer CenterWest China HospitalSichuan UniversityChengdu 610041China

Oral squamous cell carcinoma(OSCC)is known as one of the most malignant tumors with high recurrence and fatality rate.The poor tumor-targeting ability of traditional chemotherapeutic drugs has been a grand challenge for anti-OSCC therapy.Beyond that,a large quantity of tumor associated macrophages in OSCC tissues further diminish the anti-tumor effects of these drugs.Therefore,we produced a therapeutic nano drug delivery system(FA-PEG-PLA-JQ1)through encapsulating JQ1[a small-molecule inhibitor of bromodomain containing protein 4(BRD4)]into the folic acid(FA)-modified nanoparticle(PEG-PLA),which could prolong the half-life of JQ1 and target the tumor tissues.And then,JQ1 released from this nanoparticle could prevent OSCC growth inducing tumor cell apoptosis,inhibiting tumor angiogenesis and the polarization of M2 type macrophages.In conclusion,our date demonstrated the therapeutic benefits of FA-PEG-PLA-JQ1 against OSCC in vivo or in vitro,which could be a novel treatment strategy for OSCC in coming days.

关键词： folic acid Nanoparticles JQ1 Oral squamous cell carcinoma Macrophages

Dietary folic acid addition reduces abdominal fat deposition mediated by alterations in gut microbiota and SCFA production in broilers

在线全文

学校读者我要写书评

暂无评论

Animal Nutrition 2023年第1期12卷 54-62页

作者： Yanli Liu Jiantao Yang Xiaoying Liu Rui Liu Yibin Wang Xinhuo Huang Yingge Li Ruifang Liu Xiaojun Yang College of Animal Science and Technology Northwest A&F UniversityYangling 712100China Nano Vitamin Engineering Research Center of Shaanxi Province Xi'an 710000China Shaanxi Province Animal Husbandry Technology Extension Station Xi'an 710016China

Intensive selective breeding for high growth rate and body weight cause excess abdominal fat in broilers.Gut microbiota and folic acid were reported to regulate lipid metabolism.A total of 210 one-day-old broilers were divided into the control(folic acid at 1.3 mg/kg)and folic acid groups(folic acid at 13 mg/kg)to illustrate the effects of folic acid on growth performance,abdominal fat deposition,and gut microbiota,and the experiment lasted 28 d.Results revealed that dietary folic acid addition decreased abdominal fat percentage(Pfolic acid addition decreased caecal Firmicutes-to-Bacteroidetes ratio(Pacid,and propionic acid contents were found to be higher under folic acid treatment(Pacid content,and the same phenomenon was detected between propionic acid and Oscillospira and Ruminococcus(Pacid and Oscillospira were identified to be negatively associated with abdominal fat percentage(Pfolic acid reduced fat deposition in broilers by inhibiting abdominal adipocyte proliferation and differentiation,which might be mediated by changes in gut microbiota and short chain fatty acid production.

关键词： folic acid Caecal microbiota Abdominal adipocytes Short chain fatty acid Broilers

Engineering a folic acid-decorated ultrasmall gemcitabine nanocarrier for breast cancer therapy: Dual targeting of tumor cells and tumor-associated macrophages

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第3期12卷 1148-1162页

作者： Pearl Moharil Zhuoya Wan Apurva Pardeshi Jiang Li Haozhe Huang Zhangyi Luo Sanjay Rathod Ziqian Zhang Yuang Chen Bei Zhang Christian A.Fernandez Jingjing Sun Song Li Center for Pharmacogenetics Department of Pharmaceutical ScienceSchool of PharmacyUniversity of PittsburghPA 15261USA

Combination of passive targeting with active targeting is a promising approach to improve the therapeutic efficacy of nanotherapy.However,most reported polymeric systems have sizes above100 nm,which limits effective extravasation into tumors that are poorly vascularized and have dense stroma.This will,in turn,limit the overall effectiveness of the subsequent uptake by tumor cells via active targeting.In this study,we combined the passive targeting via ultra-small-sized gemcitabine(GEM)-based nanoparticles(NPs)with the active targeting provided by folic acid(FA)conjugation for enhanced dual targeted delivery to tumor cells and tumor-associated macrophages(TAMs).We developed an FAmodified prodrug carrier based on GEM(PGEM)to load doxorubicin(DOX),for co-delivery of GEM and DOX to tumors.The co-delivery system showed small particle size of~10 nm in diameter.The ligand-free and FA-targeted micelles showed comparable drug loading efficiency and a sustained DOX release profile.The FA-conjugated micelles effectively increased DOX uptake in cultured KB cancer cells that express a high level of folate receptor(FR),but no obvious increase was observed in 4T1.2 breast cancer cells that have a low-level expression of FR.Interestingly,in vivo,systemic delivery of FAPGEM/DOX led to enhanced accumulation of the NPs in tumor and drastic reduction of tumor growth in a murine 4T1.2 breast cancer model.Mechanistic study showed that 4T1.2 tumor grown in mice expressed a significantly higher level of FOLR2,which was selectively expressed on TAMs.Thus,targeting of TAM may also contribute to the improved in vivo targeted delivery and therapeutic efficacy.

关键词： folic acid Gemcitabine Polymeric micelles Breast cancer Dual targeting Tumor associated macrophages Ultrasmall nanocarrier Doxorubicin

Maternal dietary deficiencies in folic acid or choline worsen stroke outcomes in adult male and female mouse offspring

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第11期18卷 2443-2448页

作者： McCoy Clementson Lauren Hurley Sarah Coonrod Calli Bennett Purvaja Marella Agnes S.Pascual Kasey Pull Brandi Wasek Teodoro Bottiglieri Olga Malysheva Marie A.Caudill Nafisa M.Jadavji Department of Biomedical Sciences Midwestern UniversityGlendaleAZUSA College of Osteopathic Medicine Midwestern UniversityGlendaleAZUSA College of Veterinary Medicine Midwestern UniversityGlendaleAZUSA Center of Metabolomics Institute of Metabolic DiseaseBaylor Scott&White Research InstituteDallasTXUSA Division of Nutritional Sciences Cornell UniversityIthacaNYUSA Department of Neuroscience Carleton UniversityOttawaONCanada Department of Child Health College of Medicine–PhoenixUniversity of ArizonaPhoenixAZUSA

Maternal one-carbon metabolism plays an important role in early life programming.There is a well-established connection between the fetal environment and the health status of the offspring.Howeve r,there is a knowledge gap on how maternal nutrition impacts stro ke outcomes in offspring.The aim of our study was to investigate the role of maternal dietary deficiencies in folic acid or choline on stroke outcomes in 3-month-old offspring.Adult female mice were fed a folic acid-deficient diet,choline-deficient diet,or control diet 4 weeks before pregnancy.They we re continued on diets during pregnancy and la ctation.Male and female offspring were weaned onto a control diet and at 2 months of age were subjected to ischemic stroke within the sensorimotor cortex via photothrombotic damage.Mothers maintained on either a folic acid-deficient diet or choline-deficient diet had reduced levels of S-adenosylm ethionine in the liver and S-adenosylhomocysteine in the plasma.After ischemic stro ke,motor function was impaired in 3-month-old offspring from mothers receiving either a folic acid-deficient diet or choline-deficient diet compared to the animals receiving a control diet.In brain tissue,there was no difference in ischemic damage volume.When protein levels were assessed in ischemic brain tissue,there were lower levels of active caspase-3 and hypoxia-inducible factor 1α in males compared to females and betaine levels were reduced in offspring from the mothers receiving a choline-deficient diet.Our results demonstrate that a deficient maternal diet at critical time points in neurodevelopment results in worse stro ke outcomes.This study emphasizes the importance of maternal diet and the impact it can have on offspring health.

关键词： apoptosis choline metabolism folic acid ischemic stroke maternal diet motor function one-carbon metabolism stroke outcome

Characterization of folic acid,5-methyltetrahydrofolate and synthetic folinic acid in the high-affinity folate transporters:impact on pregnancy and development

在线全文

学校读者我要写书评

暂无评论

Reproductive and Developmental Medicine 2023年第2期7卷 102-107页

作者： Ana M.Palacios Rachel A.Feiner Robert M.Cabrera Department of Nutritional Sciences Dell Pediatric Research InstituteUniversity of Texas at AustinAustinTexas Department of Health Policy and Community Health Jiann Ping Hsu College of Public HealthGeorgia Southern UniversitySavannahGeorgia Department of Behavioral Neuroscience College of ScienceNortheastern UniversityBostonMassachusetts Center for Precision Environmental Health Baylor College of MedicineHoustonTexas

Objectives:Folates are B vitamins that are essential for several molecular,cellular,and biological processes,including nucleotide synthesis,methylation,and methionine cycling.The physiological impacts of these processes on health also extend to cell proliferation,folate deficiency anemia,and reduction of the risk of birth defects during pregnancy.The primary objective of this study was to characterize the binding affinities of different folate forms,folic acid(FA),5-methyltetrahydrofolate(5MTHF),and folinic acid,to the folate receptorsαandβ,and to the bovine milk folate binding protein.These three dietary forms of folate are found in enriched grains(FA),various fruits and leafy vegetables(folinic acid),and red blood cells(5MTHF).Methods:The half maximal inhibitory concentration values and binding curves of each of these folates for each receptor were determined.Results:Our results indicated that FA had the highest affinity for all folate receptors,followed by 5MTHF,and lastly,by folinic acid,examined by several orders of magnitudes.Conclusion:These data are expected to provide new insights into the therapeutic applications of the different forms of folate in a variety of diseases.

关键词： 5-methyltetrahydrofolate folic acid folinic acid folate receptorα folate receptorβ affinity

Impact of increasing one-carbon metabolites on traumatic brain injury outcome using pre-clinical models

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第8期19卷 1728-1733页

作者： Sanika M.Joshi Theresa Currier Thomas Nafisa M.Jadavji College of Osteopathic Medicine Midwestern UniversityGlendaleCAUSA Department of Biomedical Sciences Midwestern UniversityGlendaleCAUSA Department of Child Health College of Medicine–PhoenixUniversity of ArizonaPhoenixAZUSA College of Veterinary Medicine Midwestern UniversityGlendaleCAUSA Department of Neuroscience Carleton UniversityOttawaCanada

Traumatic brain injury is a major cause of death and disability worldwide,affecting over 69 million individuals yearly.One-carbon metabolism has been shown to have beneficial effects after brain damage,such as ischemic stroke.However,whether increasing one-carbon metabolite vitamins impacts traumatic brain injury outcomes in patients requires more investigation.The aim of this review is to evaluate how one-carbon metabolites impact outcomes after the onset of traumatic brain injury.PubMed,Web of Science,and Google Scholar databases were searched for studies that examined the impact of B-vitamin supplementation on traumatic brain injury outcomes.The search terms included combinations of the following words:traumatic brain injury,dietary supplementation,one-carbon metabolism,and B-vitamins.The focus of each literature search was basic science data.The year of publication in the literature searches was not limited.Our analysis of the literature has shown that dietary supplementation of B-vitamins has significantly improved the functional and behavioral recove ry of animals with traumatic brain injury compared to controls.Howeve r,this improvement is dosage-dependent and is contingent upon the onset of supplementation and whether there is a sustained or continuous delive ry of vitamin supplementation post-traumatic brain injury.The details of supplementation post-traumatic brain injury need to be further investigated.Overall,we conclude that B-vitamin supplementation improves behavioral outcomes and reduces cognitive impairment post-traumatic brain injury in animal model systems.Further investigation in a clinical setting should be stro ngly considered in co njunction with current medical treatments for traumatic brain injury-affected individuals.

关键词： folic acid nicotinamide one-carbon metabolism riboflavin traumatic brain injury vitamin B12 vitamin B2 vitamin B3

folic acid supplementation attenuates hyperhomocysteinemia-induced preeclampsia-like symptoms in rats

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2012年第25期7卷 1954-1959页

作者： Jun Wang Yan Cui Jing Ge Meijing Ma Department of Gynaecology and Obstetrics the 202 Hospital of People's Liberation Army Shenyang 110003 Liaoning Province China Department of Emergency Medicine General Hospital of Shenyang Military Region Shenyang 110016 Liaoning Province China Department of Gynaecology and Obstetrics the Second People's Hospital of Tongliao Tongliao 280000 Inner Mongolia AutonomousRegion China

folic acid participates in the metabolism of homocysteine and lowers plasma homocysteine levels directly or indirectly. To establish a hyperhomocysteinemic pregnant rat model, 2 mL of DL-homocysteine was administered daily by intraperitoneal injection at a dose of 200 mg/kg from day 10 to day 19 of gestation. folic acid was administered by intragastric administration at a dose of 20 mg/kg during the period of preeclampsia induction. Results showed that systolic blood pressure, proteinuria/creatinine ratio, and plasma homocysteine levels in the hyperhomocysteinemic pregnant rats increased significantly, and that body weight and brain weight of rat pups significantly decreased. folic acid supplementation markedly reversed the above-mentioned abnormal changes of hyperhomocysteinemic pregnant rats and rat pups. These findings suggest that folic acid can alleviate the symptoms of hyperhomocysteinemia- induced preeclampsia in pregnant rats without influencing brain development of rat pups.

关键词： folic acid preeclampsia hyperhomocysteinemia proteinuria creatinine preeclampsia-likesymptom pregnant rats offspring brain nervous system regeneration neural regeneration

folic acid Modified Electrospun Poly(vinyl alcohol)/Polyethyleneimine Nanofibers for Cancer Cell Capture Applications

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2016年第6期34卷 755-765页

作者： Zhang-yu Fan Yi-li Zhao 朱晓玥 Yu Luo Ming-wu Shen 史向阳 College of Chemistry Chemical Engineering and BiotechnologyDonghua UniversityShanghai 201620China Key Laboratory of Textile Science&Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai 201620China

Capture and detection of metastatic cancer cells are crucial for diagnosis and treatment of malignant neoplasm. Here, we report the use of folic acid （FA） modified electrospun poly（vinyl alcohol）（PVA）/polyethyleneimine （PEI） nanofibers for cancer cell capture applications. Electrospun PVA/PEI nanofibers crosslinked by glutaraldehyde vapor were modified with FA via a poly（ethylene glycol）（PEG） spacer, followed by acetylation of the fiber surface PEI amines. The formed FA-modified nanofibers were well characterized. The morphology of the electrospun PVA/PEI nanofibers is smooth and uniform despite the surface modification. In addition, the FA-modified nanofibers display good hemocompatibility as confirmed by hemolysis assay. Importantly, the developed FA-modified nanofibers are able to specifically capture cancer cells overexpressing FA receptors, which were validated by quantitative cell counting assay and qualitative confocal microscopy analysis. The developed FA-modified PVA/PEI nanofibers may be used for capturing circulating tumor cells for cancer diagnosis applications.

关键词： Poly（vinyl alcohol） Polyethyleneimine Electrospun nanofibers folic acid Cancer cell capture.