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Determination of Sulfadiazine Based on Its Derivatization with fluorescamine by Self-Ordered Ring Fluorescence Microscopic Imaging Technique

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光谱学与光谱分析 2012年第9期32卷 2458-2463页

作者： YANG Le LIU Ying College of Life and Environmental Sciences Minzu University of China Beijing 100081 China Yinchuan No. 2 High School Ningxia 750004 China

A self-ordered ring(SOR) fluorescence microscopic imaging technique has been developed for the determination of trace amounts of sulfadiazine based on its derivatization with *** the presence of HAc-NaAc buffer solution(pH 3.12) and polyvinyl alcohol-124(PVA-124),the droplet containing fluorescamine derivatized sulfadiazine can form a SOR on the solid support after solvent evaporation with the diameter of 1.86mm and its ring belt width of 54.9 μ *** quantitative analysis of sulfadiazine is achieved with the linear range of 7.8×10-14 ～1.8×10-12 mol·ring-1(3.9×10-7 ～9.0×10-6 mol·L-1) and detection limit of 7.8×10-15 mol·ring-1(3.9×10-8 mol·L-1) when 0.2 μ L droplet was *** technique has been satisfactorily applied to the determination of sulfadiazine in the tablet,synthetic sample and residues in six different milk samples with the recoveries of 91.0%～105.8%,respectively,and RSDs less than 4.4%.

关键词： SOR Sulfadiazine fluorescamine Derivatization

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Study of the interaction of Zn^(2+) with Aβ(10-21) by fluorescamine assay

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Chinese Chemical Letters 2007年第1期18卷 97-98页

作者： Chao Feng Zhang Li Fan Pin Yang Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Zinc may play a role as a co-factor in the pathogenesis of Alzheimer＇s disease （AD） through influencing the conformation and neurotoxicity of amyloid β-protein （Aβ）. Using the fluorescamine assay, we show for the first time that Zn^2＋ induced Aβ（10-21） aggregate in a concentration-dependent manner. These results indicate that Aβ（10-21） can be used as an in vitro model in Zn^2＋ - induced Aβ aggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ（1-42）.

关键词： Aβ(10-21) fluorescamine Zn^2+ Aggregation

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High Performance Liquid Chromatography for the Determina- tion of Metoclopramide in Pharmaceutical Preparations Using Pre-column Derivatization with fluorescamine

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Chinese Journal of Chemistry 2012年第3期30卷 634-638页

作者： Tatar Ulu,Sevgi Department of Analytical Chemistry Faculty of PharmacyIstanbul UniversityIstanbul34416Turkey

A simple, rapid and sensitive high performance liquid chromatography （HPLC） method was developed for the determination of metoclopramide in pharmaceutical preparation. The method is based on the derivatization of metoclopramide with fluorescamine. The separation was achieved on a C18 column using methanol-water （70 ： 30, V/V） mobile phase. Fluorescence detector was used at the excitation and emission of 403 and 485 nm, respectively. The method was validated for linearity, limit of detection, limit of quantification, precision, accuracy, recovery, robust- ness and system suitability. The assay was linear over the concentration range of 100-2000 ng/mL. The mean recovery was 100.37%. The proposed method was successfully applied to the assay of metoclopramide in tablet preparation. The preparation was also analyzed with an official method and statistical comparison by t- and F-tests revealed that there was no significant difference between the results of the two methods with respect to mean values and standard deviations at the 95% confidence level.

关键词： metoclopramide fluorescamine validation HPLC derivatization determination

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Improved speciation of dissolved organic nitrogen in natural waters: amide hydrolysis with fluorescence derivatization

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Journal of Environmental Sciences 2008年第10期20卷 1273-1280页

作者： Ryan L.Fimmen Tamara D.Trouts Daniel D.Richter Jr. Dharni Vasudevan School of Earth Sciences The Ohio State University 125 S.Oval MallColumbus OH 43210 USA.Nicholas School of the Environment and Earth Sciences Duke University Durham NC 27708 USA School of Earth Sciences The Ohio State University 125 S.Oval MallColumbus OH 43210 USA Nicholas School of the Environment and Earth Sciences Duke UniversityDurham NC 27708 USA. Nicholas School of the Environment and Earth Sciences Duke UniversityDurham NC 27708 USA.Department of Chemistry Bowdoin CollegeBrunswick ME 04011 USA

The objective of this study was to improve primary-amine nitrogen (1°-N) quantification in dissolved organic matter (DOM) originating from natural waters where inorganic forms of N, which may cause analytical interference, are commonly encountered. Efforts were targeted at elucidating organic-N structural criteria influencing the response of organic amines to known colorimetric and fluorescent reagents and exploring the use of divalent metal-assisted amide hydrolysis in combination with fluorescence analyse...

关键词： dissolved organic matter/nitrogen (DOM/DON) o-phthaldialdehyde fluorescamine ninhydrin amino acids amide hydrolysis peptides

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A high throughput sequential injection fluorometric procedure for the determination of protein in human serum

A high throughput sequential injection fluorometric procedur...

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中国化学会第十三届有机分析与生物分析学术会议

作者：陈旭伟杨梅王建华 Research Center for Analytical Sciences Northeastern UniversityShenyang 110004 Research Center for Analytical Sciences Northeastern UniversityShenyang 110004 Research Center for Analytical Sciences Northeastern UniversityShenyang 110004

＜正＞A high throughput sensitive procedure for the quantification of total protein in human serum was presented with sequential injection sampling and fluorometric detection . The flow manifold is shown in Fig. 1. A few mi-croliters of sample and fluorescamine solutions were employed to facilitate the reaction between protein and fluorescamine and give rise to a blue-green-fluorescent deri-

关键词： Protein Sequential injection Human serum fluorescamine Fluorometry

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