High Performance Liquid Chromatography for the Determina- tion of Metoclopramide in Pharmaceutical Preparations Using Pre-column Derivatization with Fluorescamine

作     者：Tatar Ulu,Sevgi 

作者机构：Department of Analytical ChemistryFaculty of PharmacyIstanbul UniversityIstanbul34416Turkey 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2012年第30卷第3期

页      面：634-638页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 070302[理学-分析化学] 0903[农学-农业资源与环境] 0703[理学-化学] 

主　　题：metoclopramide fluorescamine validation HPLC derivatization determination 

摘      要：A simple, rapid and sensitive high performance liquid chromatography （HPLC） method was developed for the determination of metoclopramide in pharmaceutical preparation. The method is based on the derivatization of metoclopramide with fluorescamine. The separation was achieved on a C18 column using methanol-water （70 ： 30, V/V） mobile phase. Fluorescence detector was used at the excitation and emission of 403 and 485 nm, respectively. The method was validated for linearity, limit of detection, limit of quantification, precision, accuracy, recovery, robust- ness and system suitability. The assay was linear over the concentration range of 100-2000 ng/mL. The mean recovery was 100.37%. The proposed method was successfully applied to the assay of metoclopramide in tablet preparation. The preparation was also analyzed with an official method and statistical comparison by t- and F-tests revealed that there was no significant difference between the results of the two methods with respect to mean values and standard deviations at the 95% confidence level.