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Aqua-ammonia pretreatment of corn stover for enhancing enzymatic saccharification

International Journal of Agricultural and Biological Engineering 2012年第1期5卷 56-61页

作者： Ruplal Choudhary Arosha L.Umagiliyage John Haddock Plant Soil and Agricultural SystemsSouthern Illinois UniversityCarbondaleIL 62901USA Microbiology Life ScienceSouthern Illinois UniversityCarbondaleIL 62901USA

Aqua-ammonia pretreatment of corn stover was provided in a 1.2 L high pressure reactor with two ammonia:biomass ratios of 1:1(w/w)and 2:1(w/w);at three temperatures:60℃,90℃and 120℃,and two treatment time:5 min and 30 *** with water was used as *** pretreated samples were saccharified to fermentable sugars by commercial enzymes ACCELERASE 1500(cellulase)and ACCELERASE XC(xylanases).For ammonia:biomass ratio of 1:1,the yield of total fermentable sugar was 87%at 90℃for 30 min *** case of ammonia:biomass ratio of 2:1,fermentable sugar yield increased four fold of that of *** fermentable sugar yield of 99%was obtained for 2:1 ammonia:biomass ratio,90℃,and 30 min treatment *** results of this study are very promising for improving fermentable sugar yield from corn stover using smaller amount of ammonia than the ammonia fiber explosion and other pretreatments such as strong acid or alkali ***,further optimization is required for reducing pretreatment time.

关键词： aqua-ammonia pretreatment corn stover fermentable sugar enzymatic saccharification biomass

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High solid and low enzyme loading based saccharification of agricultural biomass

High solid and low enzyme loading based saccharification of ...

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第八届中国酶工程学术研讨会

作者： Yu Zhang Yun Liu Jing-Liang Xu Zhen-Hong Yuan Wei Qi Xin-Shu Zhuang Key Laboratory of Renewable Energy and Gas Hydrate ＆ Guangzhou Institute of Energy Conversion Chinese Academy of Sciences

Two agricultural biomass materials namely wheat straw and sugarcane bagasse were pretreated by NaOH,and then used as substrates for enzymatic *** the pretreatment,the increase in glucan content and the decrease in lignin content was more than 65%,while less than 20%increase occurred in xylan *** enzymatic saccharification was initiated with solid loading 9%（w/v）,and then 8%,7%and 6%（w/v） solid was fed at 8,24 and 48 h,*** final enzyme solid loading was 9.6 FPU/g solid and 30%（w/v）,*** 144 h,the produced glucose,xylose and reducing sugar concentrations for wheat straw were81.88,20.30 and 115.25 g/L,respectively,and for sugarcane bagasse they were 125.97,8.66 and 169.50 g/L,*** final conversion rates of wheat straw and sugarcane bagasse were 34.57%and 50.85%,*** assay showed that the surface structure of the two materials changed a lot via alkali-pretreatment and enzymatic ***,high concentration sugar is produced from the two agricultural biomass materials by high solid and low enzyme *** to wheat straw,sugarcane bagasse is more suitably used for sugar production.

关键词： Cellulase fermentable sugar High solid loading Enzymatic saccharification Biomass

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Ameliorated enzymatic saccharification of corn stover with a novel modified alkali pretreatment

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Journal of Bioresources and Bioproducts 2016年第1期1卷 42-47页

作者： Guang Yu Huanfei Xu Chao Liu Paul DeRoussel Chunyan Zhang Yuedong Zhang Bin Li Haisong Wang Xindong Mu CAS Key Laboratory of Bio-based Materials Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao266101China College of Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042China Procter and Gamble Manufacturing Procter and Gamble Co.MasonOhio45040USA Materials Process and DeliveryProcter and Gamble Singapore Innovation CentreProcter and Gamble Co.Singapore138648Singapore

Enzymatic saccharification/hydrolysis is one of the key steps for the bioconversion of lignocelluloses into sustainable *** this work,corn stover was pretreated with a novel modified alkali process(NaOH+anthraquinone(AQ)+sodium lignosulfonate(SLS)),and then enzymatically hydrolyzed with an enzyme cocktail(cellulase(Celluclast 1.5L),β-glucosidase(Novozyme 188)and xylanase(from thermomyceslanuginosus))in the pH range of *** was found that the suitable pH for the enzymatic saccharification process to achieve a high glucan yield was between 4.2 and 5.7,while the appropriate pH to obtain a high xylan yield was in the range of *** best pH for the enzymatic saccharification process was found to be 4.4 in terms of the final total sugar yield,as xylanase worked most efficiently in the pH range of 4.0-4.7,under the conditions in the *** addition of xylanase in the enzymatic saccharification process could hydrolyze xylan in the substrates and reduce the nonspecific binding of cellulase,thus improving the total sugar yields.

关键词： Enzymatic saccharification Hydrolysis pH Xylanase Corn stover Biorefinery fermentable sugar cellulase

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