Aqua-ammonia pretreatment of corn stover for enhancing enzymatic saccharification

作     者：Ruplal Choudhary Arosha L.Umagiliyage John Haddock 

作者机构：PlantSoil and Agricultural SystemsSouthern Illinois UniversityCarbondaleIL 62901USA MicrobiologyLife ScienceSouthern Illinois UniversityCarbondaleIL 62901USA 

出 版 物：《International Journal of Agricultural and Biological Engineering》 (国际农业与生物工程学报（英文）)

年 卷 期：2012年第5卷第1期

页      面：56-61页

学科分类：0710[理学-生物学] 0810[工学-信息与通信工程] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 070303[理学-有机化学] 0703[理学-化学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：This study was financially supported by the Office of Vice Chancellor for Research SIU Carbondale via an Interdisciplinary Research Seed Grant 

主　　题：aqua-ammonia pretreatment corn stover fermentable sugar enzymatic saccharification biomass 

摘      要：Aqua-ammonia pretreatment of corn stover was provided in a 1.2 L high pressure reactor with two ammonia:biomass ratios of 1:1(w/w)and 2:1(w/w);at three temperatures:60℃,90℃and 120℃,and two treatment time:5 min and 30 *** with water was used as *** pretreated samples were saccharified to fermentable sugars by commercial enzymes ACCELERASE 1500(cellulase)and ACCELERASE XC(xylanases).For ammonia:biomass ratio of 1:1,the yield of total fermentable sugar was 87%at 90℃for 30 min *** case of ammonia:biomass ratio of 2:1,fermentable sugar yield increased four fold of that of *** fermentable sugar yield of 99%was obtained for 2:1 ammonia:biomass ratio,90℃,and 30 min treatment *** results of this study are very promising for improving fermentable sugar yield from corn stover using smaller amount of ammonia than the ammonia fiber explosion and other pretreatments such as strong acid or alkali ***,further optimization is required for reducing pretreatment time.