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Effect of short neuropeptide F signaling on larval feeding in Mythimna separata

Insect Science 2024年第2期31卷 417-434页

作者： Mei-Mei Li Qi Yang Li-Hui Chen Yan-Ying Li Jun-Xiang Wu Xiang-Li Xu State Key Laboratory of Crop Stress Biology in Arid Areas College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi ProvinceChinaand School of Agricultural Sciences Jiangxi Agricultural UniversityNanchangChina

Mythimna separata is a notorious phytophagous pest which poses serious threats to cereal crops owing to the gluttony of the larvae.Because short neuropeptide F(sNPF)and its receptor sNPFR are involved in a diversity of physiological functions,especially in functions related to feeding in insects,it is a molecular target for pest control.Herein,an sNPF and 2 sNPFRs were identified and cloned from M.separata.Bioinformatics analysis revealed that the sNPF and its receptors had a highly conserved RLRFamide C-terminus and 7 transmembrane domains,respectively.The sNPF and its receptor genes were distributed across larval periods and tissues,but 2 receptors had distinct expression patterns.The starvation-induced assay elucidated that sNPF and sNPFR expression levels were downregulated under food deprivation and recovered with subsequent re-feeding.RNA interference knockdown of sNPF,sNPFR1,and sNPFR2 by injection of double-stranded RNA into larvae not only suppressed food consumption and increased body size and weight,but also led to decrease of glycogen and total lipid contents,and increase of trehalose compared with double-stranded green fluorescent protein injection.Furthermore,molecular docking was performed on the interaction mode between sNPFR protein and its ligand sNPF based on the 3-dimensional models constructed by AlphaFold;the results indicated that both receptors were presumably activated by the mature peptide sNPF-2.These results revealed that sNPF signaling played a considerably vital role in the feeding regulation of M.separata and represents a potential control target for this pest.

关键词： feeding regulation Mythimna separata pest control target RNA interference short neuropeptide F sNPF receptors

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Dorsomedial hypothalamic nucleus neurons integrate important peripheral feeding-related signals in rats

Dorsomedial hypothalamic nucleus neurons integrate important...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Jing-Ning ZHU,Chun-Li GUO,Hong-Zhao LI,Jian-Jun WANG Department of Biological Science and Technology,School of Life Sciences,Nanjing University,Nanjing 210093,China

＜正＞Several studies have implicated the dorsomedial hypothalamic nucleus（DMN） in regulation of feeding behavior and body weight,but clear mechanisms by which it controls food intake are not well understood.Here we report that DMN received important peripheral short-term and long-term feeding-related afferent signals,including the gastric vagal inputs, glycemia,cholecystokinin（CCK） as well as leptin,an adipostatic signal forcefully inhibiting food intake and increasing metabolic rate.Among the 279 DMN neurons recorded,173（62.0%） responded to the stimulation of gastric vagal nerves. Also,of the 123 DMN neurons responsive to the gastric vagal stimulation that tested for intravenous glucose administration, 75（61.0%） were identified to be glycemia-sensitive.Moreover,it is noteworthy that within the 23 DMN neurons responding to both of the gastric vagal and intravenous glucose stimulations,most cells（19/23,82.6%） were sensitive to circulating leptin and some neurons（n = 7） were also responsive to systemic CCK,suggesting gastric vagal,glycemic,CCK and leptin inputs converge onto single DMN neurons.Furthermore,synergistic interactions between leptin and glucose on single DMN neurons were observed（n = 6）.These results demonstrate that those important peripheral feeding-related gastric vagal,glycemic,CCK and leptin signals not only reach the DMN but also interact on single DMN neurons, suggesting that the DMN may not just function as a relay station,but independently integrate the short-term and longterm feeding-associated information and actively participate in the direct regulation of feeding behavior.

关键词： dorsomedial hypothalamic nucleus leptin gastric vagal nerves glycemia-sensitive neurons feeding regulation

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