Effect of short neuropeptide F signaling on larval feeding in Mythimna separata
作者机构：State Key Laboratory of Crop Stress Biology in Arid AreasCollege of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi ProvinceChinaand School of Agricultural SciencesJiangxi Agricultural UniversityNanchangChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2024年第31卷第2期
页 面：417-434页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：funded by Special Fund for Agro-scientific Research in the Public Interest of China(201403031) the National Key R&D Program of China(2017YFD0201807)
主 题：feeding regulation Mythimna separata pest control target RNA interference short neuropeptide F sNPF receptors
摘 要：Mythimna separata is a notorious phytophagous pest which poses serious threats to cereal crops owing to the gluttony of the *** short neuropeptide F(sNPF)and its receptor sNPFR are involved in a diversity of physiological functions,especially in functions related to feeding in insects,it is a molecular target for pest ***,an sNPF and 2 sNPFRs were identified and cloned from *** analysis revealed that the sNPF and its receptors had a highly conserved RLRFamide C-terminus and 7 transmembrane domains,*** sNPF and its receptor genes were distributed across larval periods and tissues,but 2 receptors had distinct expression *** starvation-induced assay elucidated that sNPF and sNPFR expression levels were downregulated under food deprivation and recovered with subsequent *** interference knockdown of sNPF,sNPFR1,and sNPFR2 by injection of double-stranded RNA into larvae not only suppressed food consumption and increased body size and weight,but also led to decrease of glycogen and total lipid contents,and increase of trehalose compared with double-stranded green fluorescent protein ***,molecular docking was performed on the interaction mode between sNPFR protein and its ligand sNPF based on the 3-dimensional models constructed by AlphaFold;the results indicated that both receptors were presumably activated by the mature peptide *** results revealed that sNPF signaling played a considerably vital role in the feeding regulation of *** and represents a potential control target for this pest.
维普期刊数据库