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Aquaculture and Fisheries 2023年第1期8卷 67-75页

作者： Pinxian Yang Xiaoqin Li Bowen Song Ming He Chaoyi Wu Xiangjun Leng National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture Shanghai Ocean UniversityShanghai 201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai 201306China

This study investigated the effects of replacing fishmeal with Clostridium autoethanogenum protein(CAP)on growth,feed utilization and intestinal histology of largemouth bass(Micropterus salmoides).The control diet contained 350 g/kg fishmeal,and then dietary fishmeal was decreased to 300 g/kg,250 g/kg,200 g/kg,150 g/kg and 100 g/kg by CAP inclusion,respectively(FM-30,FM-25,FM-20,FM-15,FM-10).The largemouth bass(110.0±1.0 g)were fed one of the six diets for 8 *** groups of FM-30,FM-25 and FM-20 showed the similar weight gain(WG)and feed conversion ratio(FCR)to the control(P>0.05),while the WG of FM-10 group was decreased by 15.9%,and FCR increased by 0.12 when compared to the control(P0.05).In conclusion,CAP could replace 150 g/kg fishmeal in diet(350 g/kg fishmeal)without adverse effects on growth,feed utilization and intestinal histology of largemouth bass.

关键词： Largemouth bass Clostridium autoethanogenum protein Growth performance feed utilization Intestinal histology

The digestible phosphorus requirement in practical diet for largemouth bass(Micropterus salmoides)based on growth and feed utilization

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Aquaculture and Fisheries 2022年第6期7卷 632-638页

作者： Pu Wang Xiaoqin Li Zhen Xu Dong Ji Ming He JiangYu Dang Xiang-jun Leng National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityChina Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture Shanghai Ocean UniversityChina Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityChina

The present study was conducted to estimate the optimum requirement of phosphorus in practical diets for largemouth bass(Micropterus salmoides).Six iso-energetic and iso-nitrogenous diets were formulated with the supplementation of monocalcium phosphate(MCP)of 0,5,10,15,20 and 25 g/kg in basal diet containing 350 g/kg fish meal,*** digestible phosphorus of the six diets was measured as 5.7,6.7,7.8,8.7,9.3 and 10.0 g/kg,*** diet was randomly fed to triplicate groups of 25 juvenile fish(initial body weight,16.0±0.2 g)for 60 *** results showed that weight gain increased,and feed conversion ratio decreased with the increase of dietary *** dietary MCP exceeded 15 g/kg(digestible phosphorus of 8.7 g/kg),the WG and FCR were maintained at the similar *** crude ash,phosphorus and calcium contents in whole body,apparent digestibility of phosphorus and protein retention also increased with the increasing phosphorus level in *** phosphorus and calcium levels and plasma phosphorus showed no further increase when dietary MCP reached 20,20 and 10 g/kg with digestible phosphorus of 9.3,9.3 and 7.8 g/kg,***-line analysis indicated that the digestible phosphorus requirement for largemouth bass were 8.9,8.7 and 9.6 g/kg based on weight gain,feed conversion ratio and vertebral phosphorus *** conclusion,the supplementation of MCP in a diet containing 350 g/kg fish meal improved the growth and feed utilization of largemouth bass,and the digestible phosphorus requirement was suggested to be 8.7–8.9 g/kg with MCP supplementation of 15.0–15.8 g/kg.

关键词： Largemouth bass Digestible phosphorus Growth feed utilization

Growth performance,feed utilization,body and fatty acid composition of Nile tilapia (Oreochromis niloticus) fed diets containing elevated levels of palm oil

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Aquaculture and Fisheries 2017年第2期2卷 67-77页

作者： Christian Larbi Ayisi Jinliang Zhao Emmanuel Joseph Rupia Key Laboratory of Freshwater Fishery Germplasm Resources Ministry of AgricultureShanghai Ocean UniversityShanghai 201306China Key Laboratory of Exploration and utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China

This study was conducted to evaluate effects of dietary palm oil levels on growth performance,feed utilization,body composition and serum metabolites of Oreochromis *** isonitrogenous diets,32% crude protein with increasing palm oil levels of 0(Control,CTRL),2%,4%,6% and 8% were used as the major lipid source for the *** results showed that greatest weight gain,specific growth rate,and protein efficiency ratio occurred at 6% dietary palm oil *** palm oil levels significantly(Pfeed formulation for tilapia,Oreochromis niloticus.

关键词： Serum metabolites Growth feed utilization Palm oil Oreochromis niloticus

Effect of chicken haemoglobin powder on growth, feed utilization, immunity and haematological index of largemouth bass (Micropterus salmoides)

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Aquaculture and Fisheries 2020年第4期5卷 187-192页

作者： Guitao Ding Songlin Li An Wanga Naisong Chen National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China Research Centre of the Agriculture Ministry on Environmental Ecology and Fish Nutrition Shanghai Ocean UniversityShanghai20136China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai201306China

A 12-week feeding trial was conducted to evaluate the effects of replacing fish meal by chicken haemoglobin powder in largemouth bass *** isonitrogenous(48%)and isolipidic(12%)diets were formulated to replace 0%(control),9.80%,19.61%and 29.41%of fish meal with chicken haemoglobin *** diet was fed to triplicate groups of fish(initial weight:49.50±0.07 g)twice *** fish specific growth rate was significantly reduced when diet replacement level was up to 19.61%,which may be related to the feed intake and apparent digestibility coefficient of protein and amino ***,the feed efficiency ratio and protein efficiency ratio were not significantly decreased until the replacement level up to 29.41%.The activity of CCP was significantly reduced when 19.61%of fish meal was ***,the activity of lysozyme and serum protein content was significantly altered in fish fed with the diets up to 29.41%***,the red blood cell count and haemoglobin content was significantly decreased when the replacement level was up to 19.61%.In overall,the diet with 9.80%of chicken haemoglobin powder was more suitable for largemouth bass.

关键词： Chicken haemoglobin meal Growth feed utilization Non-specific immunity Largemouth bass

Effects of Using Exogenous Digestive Enzymes or Natural Enhancer Mixture on Growth, feed utilization, and Body Composition of Rabbitfish, Siganus rivulatus

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Journal of Agricultural Science and Technology(B) 2014年第3期4卷 180-187页

作者： Mahmoud A. O. Dawood Ashraf El-Dakar Mohamed Mohsen Elsayed Abdelraouf Shunsuke Koshio Manabu Ishikawa Saichiro Yokoyama Animal Production Department Faculty of Agriculture Kafrelsheikh University Kafrelsheikh 33516 Egypt Laboratory of Aquatic Animal Nutrition Faculty of Fisheries Kagoshima University Shimoarata 4-50-20 Japan Fish Resources and Aquaculture Department Suez Canal University El-Arish 43511 Egypt

This study was conducted to study the effects of a commercial exogenous digestive enzymes （EDE） containing cellulases, xylanases, u-amylase and proteases from anaerobic bacterium and natural enhancer mixture （NEM） containing allicin, high unit hydrolytic enzyme, Bacillus subtilis spores and ginseng extracts on survival, growth, carcass composition and feed cost/benefit in rabbitfish, Siganus rivulatus. Nine net cages （100 cm × 100 cm × 40 cm; L ×W ×H） were stocked with 10 juvenile rabbitfish （1.1 g per fish） each and placed in a large rectangular tank and offered feed at 4% body weight daily. Three replicates per treatment were offered three isonitrogenous and isocaloric diets, control and two supplemented diets with EDE or NEM at 2 g/kg diet for 74 days. Fish in all cages were weighed at two-week intervals and feed regimen was adjusted accordingly. Rabbitfish offered the control diet exhibited lower growth significantly （P 〈 0.05） and feed utilization than the other two experimental treatments. Experimental diet supplemented with NEM recorded the highest growth performance, feed conversion ratio, protein efficiency ratio, net protein utilization and energy retention significantly （P 〈 0.05） than the other two tested groups. Carcass composition and somatic parameters index was not affected by dietary EDE or NEM inclusion. Ultimately, when all variables are considered, EDE and NEM inclusion to diets appears to reduce feed cost per unit growth of rabbitfish.

关键词： Probiotic digestive enzymes growth feed utilization Siganus rivulatus.

Effect of Using Baker＇s Yeast and Exogenous Digestive Enzymes as Growth Promoters on Growth, feed utilization and Hematological Indices of Nile tilapia, Oreochromis niloticus Fingerlings

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Journal of Agricultural Science and Technology(B) 2012年第1期2卷 15-28页

作者： Ashraf Mohamed Abdelsamee Goda Hafez Abdel-Hamid Hassan Mabrouk Mohamed Abd EI-Hamid Wafa Tarek Mohamed E1-Afifi National Institute of Oceanography and Fisheries （NIOF） Fish Nutrition Laboratory Cairo 24135 Egypt Regional Center for Foods and feeds Agriculture Research Center Ministry of Agriculture Cairo 24135 Egypt

The present study was conducted to evaluate the effect of baker yeast, Saccharomyces cerevisiae （SC） and exogenous digestive enzymes （pepsin, papain and a-amylase, EDE） dietary supplementation on growth performance, feed utilization and hematological indices of Nile tilapia, Oreochromis niloticus fingerlings. A total of 630 Nile tilapia fingerlings with an average body weight of 26.4 ± 0.2 g were divided in the seven experimental net-pen treatments （three replicates each）. The experiment was conducted for 119 days. Seven isonitrogenous （26.50%） digestible protein and isocaloric （13.40 MJ kgl） digestible energy experimental diets were formulated. The control diet had no SC and EDE added. Diets 2-3 each contained SC at levels of 2 and 4 g 100 g diet-t, respectively, while diets 4-5 each contained EDE at levels of （0.64, 1.28, 0.16） and （1.28, 2.56, 0.32） g 100 gdiefI of pepsin, papain and a-amylase, respectively. Diet 6 contained mixture of SC and EDE at levels of 1 g yeast and 0.32, 0.64, 0.08 g of pepsin, papain and a-amylase, respectively 100 gdiet1 and diet D7 contained 2 g yeast and 0.64, 1.28, 0.16 g of pepsin, papain and a-amylase, respectively 100 g dietl. Growth performance and feed utilization efficiency of Nile tilapia were significantly （P 〈 0.05） higher in all treatments receiving SC and/or EDE supplemented-diets than the control diet which suggests that the addition of SC and EDE enhanced the growth performance. Red blood cells counts, hematocrit and hemoglobin were significantly （P 〈 0.05） highest in all treatments receiving mixture of SC and EDE supplemented-diets （D6 ＋ D7）. The same trend was observed for total plasma protein and total plasma globulin levels. The results of present study suggested that Nile tilapia fingerlings fed diets containing the mixture of I g yeast, SC and 0.32, 0.64, 0.08 g of pepsin, papain and a-amylase, respectively 100 gdiet^-1, for 119 days had enhanced growth performance, diet utilization efficiency and hematological ind

关键词： Baker yeast digestive enzymes growth feed utilization hematological indices Nile tilapia.

The Effects of Size-fractionated Fish Hydrolysate in the Diet on Growth, and feed utilization of Turbot （Scophthalmus maximus）

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feed & Livestock 2010年第3期3卷 9-9页

作者： Mengqing Liang Hongbo Yao Keke Zhang Qing chang Jia linwan Yellow Sea Fisheries Research Institute 106 Nanjing Road Qingdao 266071 P. R China

Four experimental diets were fed to turbet to examine the effect of fish bydrolyside and ultra filtered fish hydrolysafe on growth performate feed utilization and growth regulation using diets low in dietary fish meal inclusion. Diets A, B contained 3.7%,1.2% fish hydrolysate to replace fish meal respectively and the fish hydrolysate in two diets was ultra filtered to keep low molecular weight compounds. The diets A,

关键词： Tutbot Fish meal Fish hydrolysate Growth performance feed utilization

Water Intake and utilization in Mithun (Bos frontalis):Effect of Environmental Temperature,Rearing System and Concentrate feed Supplement

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Journal of Mountain Science 2008年第2期5卷 178-188页

作者： D.T.Pal A.Dhali S.K.Mondal C.Rajkhowa K.M.Bujarbaruah National Research Centre on Mithun ICAR Complex Jharnapani Medziphema Nagaland-797106 India Scientist NRC on Mithun Nagaland India Scientist (SS) Indian Veterinary Research Institute Izatnagar Bareilly UP-243122 India Director NRC on Mithun Nagaland India Deputy Director General (AS) ICAR Krishi Bhavan New Delhi India

Seasonal and sexual variations as well as the effect of dry feed supplement on total drinking water intake and its utilization were observed in mithun （Bosfrontalis） - a semi-wild animal found in North Eastern Hill Region （NEHR） of India. In a completely randomized design, twelve adult mithuns （B. frontalis） as per their sex and body weight were assigned in two different rearing systems （free grazing and free grazing with dry concentrate feed supplementation）, and ten growing male mithuns as per their body weight assigned in two different levels of dry concentrate feed supplementation （1.o kg and 2.0 kg dry concentrate feeds on green forage based diet） and in two different seasons （summer and winter）. It was observed that the environmental temperature had a significant effect on drinking water intake by mithuns. Drinking water consumption （per unit of body weight） was significantly （P 〈 0.05） higher in summer than in winter. Supplementation of concentrate feed on free grazing animals resulted in increase in water consumption. Total water consumption （drinking as well as performed water） was found to be 15.18 litres per 100 kg body weight by growing mithun. feed dry matter and digestible nutrient intakes by growing mithun were observed to be increased with the increase of supplementation of dry concentrate feed. Roughage to concentrate ratio did not affect the nutrient digestibility. Mithun calves drank an average of 4.30 litres water for each kg of dry matter intake. Metabolic water was significantly （P〈0.01） increased with the increase of supplementation of concentrate feed whereas water turn over, which depends upon the body weight of the animals, did not differ significantly on offering of lower or higher level of dry feed. Faecal water loss of growing mithun was decreased with the increase in intake of concentrate feed and was estimated to be 33 - 46 % of total water intake. Excretion of water through faeces of mithun was about 3.8 % of body weight. It could, t

关键词： Mithun water intake water excretion seasons grazing concentrate feed feed utilization

Effect of Different Dietary Protein and Lipid Levels on the Growth,Body Composition,and Intestinal Digestive Enzyme Activities of Juvenile Yellow Drum Nibea albiflora(Richardson)

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Journal of Ocean University of China 2018年第5期17卷 1261-1267页

作者： WANG Ligai HU Shenyu LOU Bao CHEN Dongxing ZHAN Wei CHEN Ruiyi LIU Feng XU Dongdong Zhejiang Marine Fisheries Research Institute Zhejiang Provincial Key Laboratory of Mariculture & Enhancement Marine and Fishery Research Institute Zhejiang Ocean University

An 8-week feeding trial was conducted to determine the optimal dietary protein-to-lipid ratio for juvenile Nibea albiflora with an initial weight of(11.76 ± 0.20) *** experimental diets containing different concentrations of protein(40%,47%,or 54%) and lipids(5%,9%,or 13%) in a 3 × 3 factorial experimental design were tested in triplicate groups of fish,while the protein-to-energy(P/E) ratios of the diets varied in the range of 19.74–28.32 mg k J^(-1).Results showed that fish fed diets containing 9% or 13% lipids with 54% protein exhibited significantly higher weight gains and specific growth rates than those fed other *** feed conversion rate of fish fed the diet with 40% protein and 5% lipids was significantly poorer than that of fish fed other *** protein efficiency rate of fish fed diets with 5% lipids was significantly lower than that of fish fed 9% or 13% lipid *** lipid and energy contents were positively correlated with dietary lipid level regardless of protein *** fed a 54% protein diet showed the highest trypsin *** intestinal lipase activity of fish fed the diet containing 13% lipids was significantly higher than that of fish fed 5% or 9% lipid *** results demonstrate the high protein dietary requirements of ***.A diet containing 54% protein and 9%–13% lipids with a P/E ratio of 26.2–27.81 mg protein k J^(-1) can be considered optimal for juvenile ***.

关键词： Nibea albiflora protein to lipid ratio growth performance feed utilization digestive enzyme activities

Effects of different protein and lipid levels on the growth performance and intestinal microflora of loach(Paramisgurnus dabryanus)

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Animal Nutrition 2023年第2期13卷 229-239页

作者： Zirui Wang Shuyao Li Qiubai Zhou Jinhua Zhang Yongan Li Youjie Li Zhiwen Yuan Guanghua Huang College of Animal Science and Technology Jiangxi Agricultural UniversityNanchang 330045China Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding Nanchang330045China

The aim of this study was to examine the effects of dietary protein and lipid levels on the growth per-formance and homeostasis of the intestinal flora in Paramisgurnus *** 8-week 3×3 two-factorial experiment was conducted to investigate the interaction between dietary crude protein(CP:30%,35%,40%)and ether extract(EE:6%,10%,14%)on the growth rate and the intestinal microflora of ***.A total of 2,160 fish(5.19±0.01 g)were randomly allotted to 36 aquariums each with 60 *** were fed the experimental diet twice *** revealed that weight gain rate(WGR),specific growth rate(SGR),protein efficiency ratio and net protein utilization significantly increased when increasing protein levels from 30%to 40%(Pfeed conversion rate,lipid efficiency ratio and net lipid utilization(P<0.05).At the phylum level,Proteobacteria and Actinobacteria were the dominant bacteria;at the genus level,Burkholderia-Caballeronia-Paraburkholderia was the dominant *** fed the diet containing 10%lipid had a higher abundance of Proteobacteria and unclassi-fied_f_Eenterobacteriaceae than those fed the 14%lipid diet,and a higher abundance of Rhodobacter than those fed the 6%lipid diet(P<0.05).Analysis of the predicted functions showed that metabolism in the intestine of fish in the CP40EE10 group was more active than that in CP30EE14 *** regression analysis found that a diet containing 40.87%protein and 9.88%lipid can be considered optimal for ***.

关键词： Paramisgurnus dabryanus Protein/lipid ratio Growth performance feed utilization Intestinal microflora