Effects of different protein and lipid levels on the growth performance and intestinal microflora of loach(Paramisgurnus dabryanus)

作     者：Zirui Wang Shuyao Li Qiubai Zhou Jinhua Zhang Yongan Li Youjie Li Zhiwen Yuan Guanghua Huang Zirui Wang;Shuyao Li;Qiubai Zhou;Jinhua Zhang;Yongan Li;Youjie Li;Zhiwen Yuan;Guanghua Huang

作者机构：College of Animal Science and TechnologyJiangxi Agricultural UniversityNanchang 330045China Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy BreedingNanchang330045China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第13卷第2期

页      面：229-239页

核心收录：

学科分类：0710[理学-生物学] 090801[农学-水产养殖] 0908[农学-水产] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by National Natural Science Foundation of China(No.31860732) China Agriculture Research System of MOF and MARA(CARS-46) Graduate Innovative Special Fund Project of Jiangxi Province(YC2021-S337) 

主　　题：Paramisgurnus dabryanus Protein/lipid ratio Growth performance Feed utilization Intestinal microflora 

摘      要：The aim of this study was to examine the effects of dietary protein and lipid levels on the growth per-formance and homeostasis of the intestinal flora in Paramisgurnus *** 8-week 3×3 two-factorial experiment was conducted to investigate the interaction between dietary crude protein(CP:30%,35%,40%)and ether extract(EE:6%,10%,14%)on the growth rate and the intestinal microflora of ***.A total of 2,160 fish(5.19±0.01 g)were randomly allotted to 36 aquariums each with 60 *** were fed the experimental diet twice *** revealed that weight gain rate(WGR),specific growth rate(SGR),protein efficiency ratio and net protein utilization significantly increased when increasing protein levels from 30%to 40%(P0.05).Both WGR and SGR enhanced first but reduced thereafter with maximum value at 10%lipid level as dietary lipid increased from 6%to 14%(P0.05).Significant interactions between protein and lipid were found with feed conversion rate,lipid efficiency ratio and net lipid utilization(P0.05).At the phylum level,Proteobacteria and Actinobacteria were the dominant bacteria;at the genus level,Burkholderia-Caballeronia-Paraburkholderia was the dominant *** fed the diet containing 10%lipid had a higher abundance of Proteobacteria and unclassi-fied_f_Eenterobacteriaceae than those fed the 14%lipid diet,and a higher abundance of Rhodobacter than those fed the 6%lipid diet(P0.05).Analysis of the predicted functions showed that metabolism in the intestine of fish in the CP40EE10 group was more active than that in CP30EE14 *** regression analysis found that a diet containing 40.87%protein and 9.88%lipid can be considered optimal for ***.