检索结果-南通市图书馆

Overexpression of S-adenosylmethionine decarboxylase(SAMDC)in Xeno-pus embryos activates maternal program of apoptosis as a "fail-safe"mechanism.of early embryogenesis

引用

Cell Research 2003年第3期13卷 147-158页

作者： MASATAKEKAI,CHIKARAKAITO,HIROSHIFUKAMACHI,TAKAYASUHIGO,EIJI TAKAYAMA,HIROSHI HARA,YOSHIKAZU OHYA,KAZUEI IGARASHI,KOICHIRO SHIOKAWA Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan Department of Parasitology National Defense Medical College Namiki 3-2 Tokorozawa Saitama 359-8843 Japan Medicinal Research Laboratories Taisho Pharmaceutical Co. Ltd. 1-403 Yoshino-cho Kita-ku Saitama 331-9530 Japan Department of Integrated Biosciences Graduate School of Frontier Sciences University of Tokyo 5-1-5 Kashiwanoha Kashiwa Chiba 277-8562 Japan Faculty of Pharmaceutical Sciences Chiba University Yayoi-cho 1-33 Inage-ku Chiba 263-8522 Japan Laboratory of Molecular Embryology Department of Biological Sciences Graduate School of Science University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-0033 Japan

In Xenopus, injection of S-adenosylmethionine decarboxylase (SAMDC) mRNA into fertilized eggs or2-cell stage embryos induces massive cell dissociation and embryo-lysis at the early gastrula stage due toactivation of the maternal program of apoptosis. We injected SAMDC mRNA into only one of the animal side blastomeres of embryos at different stages of cleavage, and examined the timing of the onset of theapoptotic reaction. In the injection at 4- and 8-cell stages, a considerable number of embryos developed intotadpoles and in the injection at 16- and 32-cell stages, all the embryos became tadpoles, although tadpolesobtained were sometimes abnormal. However, using GFP as a lineage tracer, we found that descendant cellsof the blastomere injected with SAMDC mRNA at 8- to 32-cell stages are confined within the blastocoel atthe early gastrula stage and undergo apoptotic cell death within the blastocoel, in spite of the continueddevelopment of the injected embryos. These results indicate that cells overexpress

关键词： SAMDC overexpression maternal apoptosis program processing-defective SAMDC mutant MBT fail-safe mechanism.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：