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Optimization of wheat debranning using laboratory equipment for ethanol production

International Journal of Agricultural and Biological Engineering 2014年第6期7卷 54-66页

作者： Elizabeth George Bayartoghtok Rentsen Lope G.Tabil Venkatesh Meda Department of Chemical and Biological Engineering University of SaskatchewanSaskatoonSK S7N 5A9Canada

ethanol production from starchy cereal grains is increasing rapidly due to increasing demand for alternative fuels.In Canada,wheat is the primary feedstock in ethanol plants.To improve the productivity of the ethanol plants in terms of product quality and yield,debranning of wheat grains may be employed.Debranning is advantageous in two ways.Firstly,bran removal increases the starch content of the feedstock,improving the fermentation efficiency of the ethanol plants.Secondly,bran,a valuable co-product can be used as an animal feed ingredient.In this study,experiments to optimize the debranning process were carried out using two kinds of abrasive equipment,the Satake and the TADD(tangential abrasive dehulling device)mills.Wheat samples(30 and 200 g)were debranned in the Satake mill at 1215,1412,and 1515 r/min rotational speeds,30,36,and 40 grit sizes,and 30,60,and 90 s retention times,and in the TADD mill at 900 r/min rotational speed,30,36,50,and 80 grit sizes,and 120,180,240,and 300 s retention times.In addition to debranning efficiency,the starch separation efficiencies of the two mills were calculated in different debranning conditions.In the Satake mill,the 30 g and 200 g sample size,1412 r/min and 1515 r/min rotational speeds,all grit sizes,and 60 s of retention time demonstrated the highest debranning efficiency.Correspondingly,optimal results in the TADD mill were obtained with 200 g sample size,900 r/min rotational speed,50 and 80 grit sizes,and 180 s and 240 s retention times.However,based on the experimental results,Satake mill provided better debranning values compared to the TADD mill.The starch separation efficiency values supported these results.

关键词： wheat debranning ethanol production Satake mill tangential abrasive dehulling device grit size retention time rotational speed starch separation efficiency

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Direct ethanol production from rice straw by coculture with two high-performing fungi

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Frontiers of Chemical Science and Engineering 2012年第2期6卷 139-145页

作者： Maki TAKANO Kazuhiro HOSHINO Graduate School of Science and Engineering University of Toyama Toyama 930-8555 Japan

To develop efficient and economical direct ethanol production from fine rice straw crashed mechanically, two high-performing fungi, which can secret hyperactive cellulases and/or ferment effectively various sugars, were selected from some strains belong to Mucor circinelloides preserved in our laboratory. The simultaneous saccharification and fermentation （SSF） by coculture with these fungi was investigated. The screening of high- performing fungi resulted in the selection of NBRC 4572 as an ethanol-producing fungus and NBRC 5398 as a cellulase-secreting fungus. The strain 4572 produced ethanol aerobically from glucose and xylose in high yields of 0.420 g/g at 36 h and 0.478 g/g at 60 h, respectively, but secreted fairly low cellulases. On the other hand, the strain 5398 also produced ethanol from glucose in yield of 0.340 g/g though it had a little growth in xylose culture. However, it secreted hyperactive cellulases that are essential for hydrolysis of rice straw in culture and the maximum activities of endo-β-glucanase and β-glucosi- dase were 2.11 U/L and 1.47 U/L, respectively. In SSF of rice straw by coculture with two fungi selected, the ethanol production reached 1.28 g/L after 96 h when the inoculation ratio of the strain 5398 to the strain 4572 was 9.

关键词： Mucor circinelloides ethanol production cellulase secretion SSF coculture rice straw

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Vintage Effect on the Strain Dependent Dynamics of ethanol production in Vineries of Tokaj

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Advances in Bioscience and Biotechnology 2021年第1期12卷 31-44页

作者： Zoltán Kállai Gyula Oros Research Institute for Viticulture and Oenology Tarcal Hungary Department of Genetic and Applied Microbiology University of Debrecen Debrecen Hungary Plant Protection Institute HAS Budapest Hungary

The dynamics of ethanol production of local strains of three yeast species and their ternary mixtures was examined in two Tokaj vineries. Although, the performance of them diverged significantly in first etaps of vinification—up to the utilization of half of the sugar content of grape juice—the variations vintages per vintages surpassed the strain-dependent alterations. The divergence in the latter aspect diminished during the last etap, and the ethanol concentration in young wines fermented by Saccharomyces cerevisiae, S. uvarum and Starmerella bacillaris (2 local strains of each) and their mixtures did not vary considerably (c.v. 4.2%). The vinification of grape juice performed more rapidly in fermentors inoculated with strains of S. cerevisiae, S. uvarum and St. bacillaris as well as with their mixtures than in spontaneously initiated ones by wild mycoflora in each vintage. The strains responded in different manners to conditions vintage per vintage, however, their ternary mixtures always fermented more intensively the grape juice than the strains alone. The strains affected the dynamics of alcohol production to different extents, but the alterations between them exceeded the variation between the average effects of the species. The circumstances of vinification significantly influenced the subsequent events of fermentation, but the maximum intensity of ethanol production was inversely proportional to the time required to start alcohol production (p

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Trends in Starch and Sucrose Content among Sweet Sorghum Genotypes and Implications for Sucrose and ethanol production

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Journal of Agricultural Science and Technology 2011年第2期5卷 161-167页

作者： M. M. Zhou C. A. Kimbeng T. L. Tew M. D. Shoko School of Plant Environmental and Soil Sciences LSU AgCenter Baton Rouge LA 70803 USA South African Sugarcane Research Institute Private Bag X02 Mount Edgecombe KwaZulu-Nata14300 South Africa Sugarcane Research Unit ARS USDA Houma LA 70360 USA Faculty of Agriculture and Natural Resources Africa University Box 1320 Mutare Zimbabwe

Sweet sorghum has been suggested as a feedstock into the sugarcane mills for sucrose production in Zimbabwe and Swaziland. Sweet sorghum is widely grown by subsistence farmers and matures in 3 to 6 months in February, March and April, before sugarcane harvesting begins. Sweet sorghum has low sucrose content that is difficult to extract during processing. The hypothesis of the study was that sweet sorghum was a potential feedstock to sugarcane mills for the production of sugar and ethanol. The objective of this study was to investigate the trends in starch and sucrose content of four sweet sorghum genotypes namely M337, M81-E, Theis and Topper, and evaluate the potential of sweet sorghum as a feed stock for sugar and ethanol production. The sorghum juice was collected on August 10, August 24, September 8, September 18 and October 2, 2006 and starch and sucrose content were determined. There were significant (P < 0.001) genotypes by sampling date interaction effects. Both starch and sucrose content increased with crop sampling date. Genotypes M337 and Theis were late maturing for sucrose content compared to M81-E and Topper. All genotypes except M337 produced no significant increase in starch after 101DAP. Trends in sucrose and starch content were similar, indicating the reason sucrose was difficult to extract from sweet sorghum. The impact of this study would be boosting the incomes of small scale growers who would be subcontracted by the sugar mills to produce sweet sorghum as a feedstock to the mills before sugarcane matures.

关键词： Sweet sorghum genotypes starch sucrose sugar processing ethanol production sampling dates

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Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

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Science Bulletin 2012年第Z2期 3782-3789页

作者： DONG HuiNa 1,2,4,5,ZHAO XueMing 2,4,5,MA YuanYuan 1* & ZHANG MinHua 1,3 1 Tianjin University R&D Center for Petrochemical Technology,Tianjin University,Tianjin 300072,China 2 Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China 3 State Key Laboratory of Engines,Tianjin University,Tianjin 300072,China 4 Key Laboratory of Systems Bioengineering,Ministry of Education,Tianjin University,Tianjin 300072,China 5 Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology,Tianjin University,Tianjin 300072,China

A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman(PB) design and response surface methodology(RSM).The effects of 19 medium components were investigated by PB design.Eight of these components were determined to have significant effects on ethanol production.The statistical model was constructed via central composite design(CCD) using five selected variables,including xylose,trisodium citrate,choline chloride,pyridoxine,and thiamine.The validity of the developed model was verified.The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium.The optimized medium was then simplified to give the final synthetic medium(S2),in which the ethanol concentration was 20.7% higher than in the RM medium.Xylose and trisodium citrate were the key medium components influencing ethanol production,while calcium pantothenate was the only growth factor required by recombinant Z.mobilis.ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.

关键词： recombinant Zymomonas mobilis synthetic medium ethanol production response surface methodology trisodium citrate xylose

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Enhancing short-term ethanol-type fermentation of waste activated sludge by adding saccharomycetes and the implications for bioenergy and resource recovery

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Journal of Environmental Sciences 2022年第3期34卷 179-189页

作者： Linke Zheng Ying Xu Hui Geng Xiaohu Dai State Key Laboratory of Pollution Control and Resource Reuse School of Environmental Science and EngineeringTongji UniversityShanghai 200092China East China Architecture Design&Research Institute Shanghai 200002China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China

ethanol-type sludge fermentation has recently attracted much attention because it can enhance direct interspecies electron transfer and thus improve the anaerobic digestion of waste activated sludge(WAS). In this paper, the enhancement of short-term ethanol-type fermentation of WAS via adding Saccharomyces was investigated. The experimental results show that the maximum ethanol production of 1030.8 ± 20.6 mg/L was achieved, with the optimum fermentation conditions of a p H of 5.1, temperature of 26.0 ℃ and time of 8.0 hr. Although the content of volatile fatty acid(VFA) increased within 10 hr, it is one order of magnitude lower than the content of ethanol, indicating that the VFA generation did not affect the efficient production of ethanol. The analyses of changes in the microbial community during the fermentation process demonstrate that the greatest Saccharomyces activity occurred in the first 8 hr and it can play an important role in ethanol production even at a very low relative abundance. Meanwhile, most typical acid-producing bacteria were inhibited, but the hydrogenotrophic methanogens(i.e., Methanobacterium) were enriched to a certain extent. Further statistical analyses reveal that the Rhodobacter, Thermomonas, Terrimonas and Saccharomyces are responsible for ethanol production during the fermentation. However, these findings not only provide a reference for the development of enhancing ethanol-type fermentation of sludge, but also are expected to provide a new way of thinking for the efficient bioenergy and resource recovery from sludge.

关键词： Anaerobic digestion Sewage sludge Methane production ethanol production Biodegradability

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Conversion of soy molasses,soy solubles,and dried soybean carbohydrates into ethanol

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International Journal of Agricultural and Biological Engineering 2013年第1期6卷 62-68页

作者： Craig C.Long William R.Gibbons Biology and Microbiology Department South Dakota State UniversityBrookingsSD57007 USA

Soy molasses and soy solubles are byproducts of the conventional soy protein concentrate and soy protein isolate manufacturing processes,respectively.Conversion of the carbohydrates in these byproducts into ethanol was examined.Standardized amounts of commercial cellulase enzymes(Novozyme cellulase,β-glucosidase,and pectinase)were added to soy molasses and soy solubles solutions prepared at various solid loading rates(33%,50%,60%,75%,and 80%)to hydrolyze oligosaccharides,followed by fermentation for 96 h using Saccharomyces cerevisiae NRRL Y-2034 and Scheffersomyces stipitis NRRL Y-7124.ethanol-extracted soybean meal(SBM)carbohydrates were also fermented for 96 h without enzymes.S.cerevisiae and S.stipitis produced about 12.5-45.0 g/L and 6.0-28.0 g/L ethanol,respectively,on molasses and solubles across these solid loading rates.The S.stipitis produced about 6.5-17 g/L ethanol and S.cerevisiae produced about 6.5-22 g/L ethanol on ethanol-extracted carbohydrates.

关键词： ethanol production soy molasses soy solubles oligosaccharides

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Potential production and spatial distribution of hybrid poplar as a biofuel crop in Connecticut,USA

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International Journal of Agricultural and Biological Engineering 2014年第2期7卷 10-18页

作者： Zhao Xue Liqin Qu Xiusheng Yang Department of Natural Resources and the Environment University of ConnecticutStorrsCT 06269-4087USA

The objective of this study was to assess the biomass production potential from hybrid poplars using marginal lands in the state of Connecticut,USA.A land-use suitability model was developed to identify and classify marginal lands in the state that could be used for growing hybrid poplars as a biofuel woody energy crop.The model was built on a geographic information system(GIS)platform,consisting of an exclusion area section,an ecological suitability section,and an economic/land-use suitability section.The model then was used to estimate the total biomass of the land-cover forests,annual biomass from forest and agricultural residues,and in particular the production potential of biomass from hybrid poplars over marginal lands in the state at county level.The results indicated that about 50%of the land in this state is unavailable for hybrid poplar cultivation and that less than 5%is highly suitable.The amount of usable area is highly variable on the county level.Without large-scale land use change,it appears that biofuel production in this state can only be a supplemental resource to the current energy supply.

关键词： biofuel plants hybrid poplar spatial distribution ethanol production Connecticut suitability model ecological suitability

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