Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

作     者：DONG HuiNa 1,2,4,5,ZHAO XueMing 2,4,5,MA YuanYuan 1* & ZHANG MinHua 1,3 1 Tianjin University R&D Center for Petrochemical Technology,Tianjin University,Tianjin 300072,China 2 Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China 3 State Key Laboratory of Engines,Tianjin University,Tianjin 300072,China 4 Key Laboratory of Systems Bioengineering,Ministry of Education,Tianjin University,Tianjin 300072,China 5 Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology,Tianjin University,Tianjin 300072,China 

出 版 物：《Science Bulletin》 (科学通报(英文版))

年 卷 期：2012年第Z2期

页      面：3782-3789页

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the National Basic Research Program of China (2011CBA00804) the National Natural Science Foundation of China(30900033,20806055,20875068) Project of Introducing Talents of Discipline to Universities (B06006) 

主　　题：recombinant Zymomonas mobilis synthetic medium ethanol production response surface methodology trisodium citrate xylose 

摘      要：A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman(PB) design and response surface methodology(RSM).The effects of 19 medium components were investigated by PB *** of these components were determined to have significant effects on ethanol *** statistical model was constructed via central composite design(CCD) using five selected variables,including xylose,trisodium citrate,choline chloride,pyridoxine,and *** validity of the developed model was *** ethanol concentration in the optimized medium was 6.7% higher than in the RM *** optimized medium was then simplified to give the final synthetic medium(S2),in which the ethanol concentration was 20.7% higher than in the RM *** and trisodium citrate were the key medium components influencing ethanol production,while calcium pantothenate was the only growth factor required by recombinant *** production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.