检索结果-南通市图书馆

Use of adenovirus vector expressing the mouse full estrogen receptor alpha gene to infect mouse primary neurons

Neural Regeneration Research 2010年第15期5卷 1138-1143页

作者： Xiao Hu Lei Lou Jun Yuan Xing Wan Jianyi Wang Xinyue Qin Department of Neurology the First Affiliated Hospital of Chongqing Medical University Chongqing 400016 China Department of Neurology Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China Department of Pediatrics Zunyi Medical College Zunyi 563003 Guizhou Province China Central Laboratory Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China Department of Oncology Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China Department of Pediatrics Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China

estrogen plays important regulatory and protective roles in the central nervous system through estrogen receptor a mediation. Previous studies applied eukaryotic expression and lentiviral vectors carrying estrogen receptor a to cladfy the underlying mechanisms. In the present study, an adenovirus vector expressing the mouse full estrogen receptor a gene was constructed to identify biological characteristics of estrogen receptor a recombinant adenovirus infecting nerve cells. Primary cultured mouse nerve cells were first infected with estrogen receptor a recombinant adenovirus at various multiplicities of infection, followed by 100 multiplicity of infection. Results showed overexpression of estrogen receptor α mRNA and protein in the infected nerve cells. estrogen receptor a recombinant adenovirus at 100 multiplicity of infection successfully infected neurons and upregulated estrogen receptor a mRNA and protein expression.

关键词： estrogen receptor a adenovirus neural cell C57BL/6 mice gene transfection nerve factor neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of fulvestrant on biological activity and Wnt expression in rat GH3 cells

引用

Neural Regeneration Research 2012年第4期7卷 283-289页

作者： Jiwei Bai Yan Wang Chuzhong Li Yazhuo Zhang Beijing Neurosurgical Institute Capital Medical University Beijing 100050 China Center of Clinical Genetics Affiliated Bayi Children＇s Hospital General Hospital of Beijing Military Command of Chinese PLA Beijing 100700 China

The present study investigated the influence of anti-estrogen treatment （fulvestrant） on pituitary adenoma cell line GH3 biological activity, the estrogen receptor a pathway, the WnT pathway, and mechanisms of decreased Wnt inhibitory factor-1 expression in GH3 cells. Results showed that fulvestrant suppressed GH3 cell proliferation and reduced hormone secretion in a dose-dependent manner. estrogen receptor a and Wnt4 expression decreased, but Wnt inhibitory factor-1 expression increased in a dose-dependent manner following fulvestrant treatment, and β-catenin expression remained unchanged. Inhibitors of DNA methylation and histone modification upregulated Wnt inhibitory factor-1 expression. Results suggested that fulvestrant suppressed biological activity of GH3 cells via the estrogen receptor a and Wnt pathways. These results suggested that decreased Wnt inhibitory factor-1 expression in GH3 cells played a role in epigenetic mechanisms. Anti-estrogen therapies could provide novel treatments for growth hormone adenomas.

关键词： β-catenin estrogen estrogen receptor a GH3 cell line Wnt inhibitory factor-1 Wnt4

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：