Use of adenovirus vector expressing the mouse full estrogen receptor alpha gene to infect mouse primary neurons

作     者：Xiao Hu Lei Lou Jun Yuan Xing Wan Jianyi Wang Xinyue Qin 

作者机构：Department of Neurology the First Affiliated Hospital of Chongqing Medical University Chongqing 400016 China Department of Neurology Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China Department of Pediatrics Zunyi Medical College Zunyi 563003 Guizhou Province China Central Laboratory Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China Department of Oncology Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China Department of Pediatrics Guizhou Provincial People's Hospital Guiyang 550002 Guizhou Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2010年第5卷第15期

页      面：1138-1143页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Research Project of Science and Technology Department of Guizhou Province No.sy3054 

主　　题：estrogen receptor a adenovirus neural cell C57BL/6 mice gene transfection nerve factor neural regeneration 

摘      要：Estrogen plays important regulatory and protective roles in the central nervous system through estrogen receptor a mediation. Previous studies applied eukaryotic expression and lentiviral vectors carrying estrogen receptor a to cladfy the underlying mechanisms. In the present study, an adenovirus vector expressing the mouse full estrogen receptor a gene was constructed to identify biological characteristics of estrogen receptor a recombinant adenovirus infecting nerve cells. Primary cultured mouse nerve cells were first infected with estrogen receptor a recombinant adenovirus at various multiplicities of infection, followed by 100 multiplicity of infection. Results showed overexpression of estrogen receptor α mRNA and protein in the infected nerve cells. Estrogen receptor a recombinant adenovirus at 100 multiplicity of infection successfully infected neurons and upregulated estrogen receptor a mRNA and protein expression.