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Journal of Genetics and Genomics 2019年第11期46卷 547-549页

作者： Jing Wang Qinghua Shi Xianrui Guo Fangpu Han Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China State Kay Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Durum wheat(Triticum durum Desf.,2n=28,AABB),which is mainly used to make pasta,is the second cultivated wheat variety worldwide.However,the durum wheat production is severely affected by a number of diseases,such as Fusarium head blight(FHB)and stem rust(Klindworth et al.,2017;Zhao et al.,2018).Thinopyrum elongatum(2n=4x=28),a close relative of wheat.

关键词： establishment characterization Thinopyrum

establishment of wheat-Thinopyrum ponticum translocation lines with resistance to Puccinia graminis f. sp. tritici Ug99

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Journal of Genetics and Genomics 2019年第8期46卷 405-407页

作者： Hongwei Li Willem H.P.Boshoff Zacharias A.Pretorius Qi Zheng Bin Li Zhensheng Li State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing100101China Department of Plant Sciences University of the Free StateBloemfontein9300South Africa

Stem rust, caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), is a destructive wheat disease and has the potential to cause significant yield losses (Olivera et al., 2015;Soko et al., 2018). The emergence of Pgt race TTKSK, virulent to the widely deployed stem rust resistance gene Sr31 and originally named as isolate Pgt-Ug99 (Pretorius et al., 2000).

关键词： establishment wheat-Thinopyrum ponticum

establishment and identification of induced pluripotent stem cells in liver cancer patients

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Asian Pacific Journal of Tropical Medicine 2014年第4期7卷 253-256页

作者： Da-Ming Zhang Jian-Jun Li Peng Yan Jian-Ting Hu Department of Laparoscopic Surgery People's Hospital of Zhengzhou

Objective:To induce pluripotent stem(IPS)cells from fibrocytes that are separated from liver cancer patients.Methods:The fibrocytes were reprogrammed to IPS cells by lentiviral vector,stained and identified by immunohistochemistry.Results:The IPS cells were successfully established from fibrocytes after infection,and IPS cell clones formed in round shape under a microscopy.The induction rate was 0.013%±0.007%.No tumor formed at the back of nude mice within 8 weeks after the inoculation of cell clone.However,tetatoma appeared in nude mice within 1 week after IPS inoculation.A few tumors formed in nude mice within 4 weeks after the inoculation of cell clones.However,subcutaneous tumors formed within 1 week after IPS inoculation.The induced IPS cells showed three germ layers in tetatoma.Nanog and OCT4 in the induced IPS cells showed hypomethylation.SSEA-A,TRA-1-6-,TRA-1-81 and Nanog were highly expressed in the induced IPS cells,indicating the IPS cells possessed the similar ability as the stem cells.Conclusion:The IPS cells of liver cancer patients can be established effectively from fibrocytes and can be cultured stably in vitro,which provides an approach for the treatment of intermediate or advanced stage liver cancer.

关键词： Fibrocyte Liver cancer Induced pluripotent stem cells establishment

establishment and evaluation of a theater influenza monitoring platform

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Military Medical Research 2018年第2期5卷 111-116页

作者： Jian Wang Hui-Suo Yang Bing Deng Meng-Jing Shi Xiang-Da Li Qing-Gong Nian Wen-Jing Song Feng Bing Qing-Feng Li Center for Disease Prevention and Control of Beijing Military Region 66th Heishitou RoadShijingshan DistrictBeijing 100042China

Background:Influenza is an acute respiratory infectious disease with a high incidence rate in the Chinese army,which directly disturbs military training and affects soldiers’health.Influenza surveillance systems are widely used around the world and play an important role in influenza epidemic prevention and control.Methods:As a theater centers for disease prevention and control,we established an influenza monitoring platform(IMP)in 2014 to strengthen the monitoring of influenza-like illness and influenza virus infection.In this study,we introduced the constitution,influenza virus detection,and quality control for an IMP.The monitoring effect was also evaluated by comparing the monitoring data with data from national influenza surveillance systems.The experiences and problems associated with the platform also were summarized.Results:A theater IMP was established based on 3 levels of medical units,including monitoring sites,testing laboratories and a checking laboratory.A series of measures were taken to guarantee the quality of monitoring,such as technical training,a unified process,sufficient supervision and timely communication.The platform has run smoothly for 3 monitoring years to date.In the 2014–2015 and 2016–2017 monitoring years,sample amount coincided with that obtained from the National Influenza Surveillance program.In the 2015–2016 monitoring year,due to the strict prevention and control measures,an influenza epidemic peak was avoided in monitoring units,and the monitoring data did not coincide with that of the National Influenza Surveillance program.Several problems,including insufficient attention,unreasonable administrative intervention or subordination relationships,and the necessity of detection in monitoring sites were still observed.Conclusion:A theater IMP was established rationally and played a deserved role in the prevention and control of influenza.However,several problems remain to be solved.

关键词： Influenza monitoring Military establishment Evaluation

Actin Polymerization Mediated by AtFH5 Directs the Polarity establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis

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Molecular Plant 2018年第11期11卷 1389-1399页

作者： Chang Liu Yi Zhang Haiyun Ren Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life ScienceBeijing Normal UniversityBeijing 100875China

The process of pollen germination is crucial for flowering plant reproduction,but the mechanisms through which pollen grains establish polarity and select germination sites are not well understood.In this study,we report that a formin family protein,AtFH5,is localized to the vesicles and rotates ahead of Lifeact-mEGFPlabeled actin filaments during pollen germination.The translocation of AtFH5 to the plasma membrane initiates the assembly of a collar-like actin structure at the prospective germination site prior to germination. Genetic and pharmacological evidence further revealed an interdependent relationship between the mobility of AtFH5-1 abeled vesicles and the polymerization of actin filaments:vesicle-localized AtFH5 promotes actin assembly,and the polymerization and elongation of actin filaments,in turn,is essential for the mobility of AtFH5-1 abeled vesicles in pollen grains.Taken together,our work revealed a molecular mechanism underlying the polarity establishment and vesicle mobility during pollen germination.

关键词： Chinese cabbage Pollen germination Polarity establishment Vesicle trafficking Actin Formin

Soil resource availability and its effect on the ecophysiology and establishment of Stipa pulchra

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Journal of Plant Ecology 2019年第4期12卷 603-614页

作者： Robert L.Fitch Erin J.Questad Edward G.Bobich Department of Biological Sciences California State Polytechnic UniversityPomona3801 West Temple Avenue PomonaCA 91768USA

Aims The objectives of this study were to(i)determine the importance of soil moisture and soil nitrogen availability as resource requirements for the establishment of Stipa pulchra,(ii)to examine how changes in slope within a local habitat can affect the accumulation of soil moisture and soil nitrogen and(iii)to measure the physiological re-sponses of S.pulchra among such slope locations.Methods The field experiment occurred in the San JoséHills of Southern California on the California State Polytechnic University,Pomona campus.Plots were established within three different slope loca-tions(shallow,moderate and steep)within four separate canyons.Three nitrogen treatments(ambient,addition and removal)were replicated within each slope location for all four canyons,and five S.pulchra plants were planted in each plot.Soil nitrogen accumula-tion and soil moisture,as well as other abiotic variables,were meas-ured across the varied slope locations and nitrogen treatments.We measured the cover,stress,and reproduction of S.pulchra plants for 2 years and used a mesocosm experiment to determine how S.pul-chra responds to varying water and nitrogen availability.Important Findings Stipa pulchra had a strong positive response to the wettest watering treatment and a weak response to nitrogen addition in the meso-cosm experiment.In the field experiment,S.pulchra responded positively in moderate slope locations and did not respond to ni-trogen treatments,possibly due to drought.Field patterns of soil moisture were driven by slope,soil compaction and solar radi-ation.Soil nitrogen accumulation did not vary among slope lo-cations.For restoration of S.pulchra,priority should be placed on managing soil moisture availability and determining suitable edaphic factors.

关键词： establishment ecophysiology slope soil moisture soil properties nitrogen deposition

establishment and Application of China Pituitary Disease Register Network

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Chinese Medical Journal 2017年第12期130卷 1512-1512页

作者： Cong-Xin Dai Ren-Zhi Wang Ming Feng Department of Neurosurgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China

To the Editor： China is the most populous country and has the largest number of clinical cases of pituitary diseases in the world. However, in the past few years, the work of clinical data collection and analysis was very weak, which lagged very far behind the developed country. To fiirther obtain the complete infornlation about Chinese patients with pituitary disease, the China Pituitary Disease Register Network （CPDRN） was established by Peking Union Medical Hospital in 2015. The CPDRN not only provides a perfect database for clinical data collection and infornlation sharing but also raises people＇s attention to the collection of clinical intb,＇mation.

关键词： establishment Application Network

First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2

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World Journal of Gastroenterology 2023年第41期29卷 5683-5698页

作者： Ning-Zu Jiang Ming-Zhen Bai Chong-Fei Huang Ze-Long Ma Ru-Yang Zhong Wen-Kang Fu Long Gao Liang Tian Ning-Ning Mi Hai-Dong Ma Ya-Wen Lu Zi-Ang Zhang Jin-Yu Zhao Hai-Ying Yu Bao-Ping Zhang Xian-Zhuo Zhang Yan-Xian Ren Chao Zhang Yong Zhang Ping Yue Yan-Yan Lin Wen-Bo Meng The First Clinical Medical College Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of General Surgery The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Laboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of General Surgery The First Hospital of Lanzhou University and Laboratory of Biological Therapy and Regenerative Medicine Transformation Gansu ProvinceLanzhou 730000Gansu ProvinceChina

BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were posi

关键词： Carcinosarcoma Drug resistance Xenograft Cell line establishment

establishment and Optimization of HPLC-MS/MS for Detection of Progesterone Residue in Raw Milk

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Agricultural Biotechnology 2019年第1期8卷 80-82,85页

作者： Zhen ZHANG Jun ZHANG Lei YAN Yuxi ZHAO Aifeng ZHANG Yuefei YAN Henan Dairy Herd Improvement Center Key Laboratory of Immunology of Ministry of Agriculture Henan Academy of Agricultural Sciences Henan Province International Joint Laboratory of Breeding Healthy Dairy Cows Henan Qianxiang Agriculture and Animal Husbandry Technology Co.Ltd. Puyang Natural Forest Husbandry Ranch Science & Technology Development Co. Ltd. Henan Cattle Breeding Genetic Determination Center

In order to establish an HPLC-MS/MS method for detecting progesterone in raw milk,raw milk was extracted with methanol,and then determined by HPLC-MS/MS after the purification with a C18 solid phase extraction column. Qualitative determination was performed according to retention time and selective ion abundance ratio,and quantification was performed by external standard method. The results showed that progesterone had a good linear relation in the range of0. 1-5. 0 ng/ml,with a correlation coefficient of 0. 999 9. The detection limit of the established method was 0. 02 μg/kg,and the quantification limit was0. 04 μg/kg. In the standard addition range of 0. 1-5. 0 ng/ml,the recovery ranged from 79. 6% to 105. 1%,with relative standard deviation≤8. 9%. Compared with the industry standard,the detection limit and the quantification limit of the established method are significantly reduced,and the sensitivity of the method is enhanced,so this method is suitable for the detection of progesterone residue in fresh milk in pastures.

关键词： HPLC-MS/MS Fresh and raw milk Progesterone Method establishment Optimization

Review of Marxist Political Party Theory Innovation Over the 70 Years Since the establishment of PRC

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学术界 2019年第11期 215-224页

作者： Meng Pengfei School of Marxism Southwest University

since the establishment of the People’s Republic of China,the central leader-groups from generation to generation have been continuously exploring and innovating Marxist political party theory in terms of its value target,ideological construction,working style,and organization developments.This process can be categorized into five stages:the founding stage in Mao Zedong’s era,the development stage in Deng Xiaoping’s ear,the promotion stage in Jiang Zemin’s times,the deepening stage under Hu Jintao’s leadership,and the stage of further innovation by Xi Jinping in the new era,with the characteristics of inheritance,development as well as scientificity and valuation.

关键词： 70years since the establishment of New China Marxist political party theory progress characteristics