Establishment and Optimization of HPLC-MS/MS for Detection of Progesterone Residue in Raw Milk

作     者：Zhen ZHANG Jun ZHANG Lei YAN Yuxi ZHAO Aifeng ZHANG Yuefei YAN 

作者机构：Henan Dairy Herd Improvement Center Key Laboratory of Immunology of Ministry of AgricultureHenan Academy of Agricultural Sciences Henan Province International Joint Laboratory of Breeding Healthy Dairy Cows Henan Qianxiang Agriculture and Animal Husbandry TechnologyCo.Ltd. Puyang Natural Forest Husbandry Ranch Science & Technology Development Co.Ltd. Henan Cattle Breeding Genetic Determination Center 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2019年第8卷第1期

页      面：80-82,85页

学科分类：09[农学] 

基　　金：Supported by Science and Technology Open Cooperation Project of Henan Province(162106000017) Science and Technology People-benefiting Plan Project of Henan Province(152207110004) Major Science and Technology Program of Henan Province(16110051020) Puyang Science and Technology Plan Project(160215) 

主　　题：HPLC-MS/MS Fresh and raw milk Progesterone Method Establishment Optimization 

摘      要：In order to establish an HPLC-MS/MS method for detecting progesterone in raw milk,raw milk was extracted with methanol,and then determined by HPLC-MS/MS after the purification with a C18 solid phase extraction column. Qualitative determination was performed according to retention time and selective ion abundance ratio,and quantification was performed by external standard method. The results showed that progesterone had a good linear relation in the range of0. 1-5. 0 ng/ml,with a correlation coefficient of 0. 999 9. The detection limit of the established method was 0. 02 μg/kg,and the quantification limit was0. 04 μg/kg. In the standard addition range of 0. 1-5. 0 ng/ml,the recovery ranged from 79. 6% to 105. 1%,with relative standard deviation≤8. 9%. Compared with the industry standard,the detection limit and the quantification limit of the established method are significantly reduced,and the sensitivity of the method is enhanced,so this method is suitable for the detection of progesterone residue in fresh milk in pastures.