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A mini-review on the enzyme-mediated manipulation of proteins/peptides

Chinese Chemical Letters 2018年第7期29卷 1017-1021页

作者： Shaomin Lin Chunmao He School of Chemistry and Chemical Engineering South China University of Technology

Recent years have seen an ever increasing number of enzyme mediated protein/peptide modification reactions, which contribute significantly to the elucidation of related biological functions. The many available enzymes have, however, caused difficulties for practitioners in choosing the most appropriate enzyme for a certain purpose. This review surveyed the widely used enzymes(i.e., sortases, butelase 1,subtiligase, formylglycine generating enzyme and farnesyltransferase) in the manipulation of proteins/peptides, and the application fields of these enzymes as well as the advantages and limitations of each enzyme are summarized.

关键词： Protein modification Protein labeling Protein ligation Site-selective enzyme-mediated

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Insertion Mutagenesis of Trichoderma atroviride by Restriction enzyme-mediated DNA integration

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Journal of Shanghai Jiaotong university(Science) 2005年第S1期10卷 161-166,175页

作者：黄玉茜周晓英陈捷梁春浩范海延徐书法程根武刘海南 School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 ChinaDepart of Plant ProtectionShenyang Agricultural Univ. Shenyang 110161 School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China Institute of Plant Protection Liaoning Academy of Agricultural Sciences Shenyang 110161 Depart of Plant Protection Shenyang Agricultural Univ. Shenyang 110161

Restriction enzyme-mediated integration(REMI) of DNA has been recently received attention as a new technique for the generation of mutants by transformation in fungi. Trichoderma atroviride strain T23 was transformed with linearized plasmid pV2, conferring resistance to hygromycin B, in the presence of restriction enzyme used to linearize the plasmid. A total of 172 regeneration transformants were detected by successive inoculation for seven times subcultivation on fresh PDA plate containing hygromycin B. The plasmid was integrated stably into the chromosome DNA, which was confirmed by PCR and southern analysis. The difference between 172 transformants and the parent strain was confirmed in colonial color, sporulation and growth rate. The results showed that the significant difference appeared in above mentioned characters between transformants and parent strain is sporulation capability. Transformants TC6, TD5, TE7, TF1 and TK1 produced higher amounts of conidia than the parent strain T23. In addition, transformants TK1and TC6 showed stronger inhibition to the growth rate of the cucumber wilt pathogen (Fusarium oxyporum) in vitro.

关键词： Trichoderma atrovride restriction enzyme-mediated integration hygromycin resistance

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Preparation of Molecularly Imprinted Composites Initiated by Hemin/Graphene Hybrid Nanosheets and Its Application in Detection of Sulfamethoxazole

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Current Medical Science 2019年第1期39卷 159-165页

作者： Shao-fei GUO Xiao-yu CHEN Peng WANG Cheng CHEN Rui-hua PAN Yue-tao LING Yi-zhu TANG Hubei Entry-Exit Inspection and Quarantine Bureau of PRC Wuhan 430022China Hubei Institute of Sport Science Wuhan 430205China

Molecularly imprinted polymers(MIPs)exhibit high selectivity resulting from imprinted cavities and superior performance from functional materials,which have attracted much attention in many ***,the combination of MIPs film and functional materials is a great *** this study,hemin/graphene hybrid nanosheets(H-GNs)were used to initiate the imprinted polymerization by catalyzing the generation of free ***,MIPs using sulfamethoxazole as the template was directly prepared on the surface of H-GNs without any film *** importantly,the template could be absorbed on the H-GNs to enhance the number of imprinted sites per unit surface area,which could improve the selectivity of MIPs ***,the composites could exhibit high adsorption capacity(29.4 mg/g),imprinting factor(4.2)and excellent conductivity,which were modified on the surface of electrode for rapid,selective and sensitive detection of sulfamethoxazole in food and serum *** linear range was changed from 5μg/kg to 1 mg/g and the limit of detection was 1.2μg/*** sensor was free from interference caused by analogues of sulfamethoxazole,which provides a novel insight for the preparation of MIPs-based sensor and its application in food safety monitoring and human exposure study.

关键词： MIP/H-GNs composites sulfamethoxazole nano enzyme-mediated polymerization sensor dietary exposure

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