A mini-review on the enzyme-mediated manipulation of proteins/peptides

作     者：Shaomin Lin Chunmao He 

作者机构：School of Chemistry and Chemical EngineeringSouth China University of Technology 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2018年第29卷第7期

页      面：1017-1021页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：The financial support from the National Recruitment Program of Global Youth Experts(1000 Talents Plan) the National Natural Science Foundation of China (No. 81703406) 

主　　题：Protein modification Protein labeling Protein ligation Site-selective Enzyme-mediated 

摘      要：Recent years have seen an ever increasing number of enzyme mediated protein/peptide modification reactions, which contribute significantly to the elucidation of related biological functions. The many available enzymes have, however, caused difficulties for practitioners in choosing the most appropriate enzyme for a certain purpose. This review surveyed the widely used enzymes(i.e., sortases, butelase 1,subtiligase, formylglycine generating enzyme and farnesyltransferase) in the manipulation of proteins/peptides, and the application fields of these enzymes as well as the advantages and limitations of each enzyme are summarized.