检索结果-南通市图书馆

pSVPoMCAT微基因修饰雪旺氏细胞移植治疗实验性脊髓损伤的酶及免疫组化评价(英文)

中国组织工程研究 2002年第6期6卷 906-907页

作者：陈礼刚朴永旭毛伯镛高立达卢敏曾凡俊王方 General Hospital of Chengdu Military Command Chengdu 610083 China Huaxi Hospital Sichuang University Chengdu 610041 China

Objective To provide morphological evidence for therapeutic effects of microgene pSVPoMcat implanted to modify schwann cells(SC) on the experimental spinal cord injury(SCI).Methods Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically SC(group A),SC implanted group(group B) and the control group(group C),each groups having 10 ***(AchE) and acid phosphatase(ACP)activity of anterior horn cells in the A,B,C groups were measured respectively with the quantitative enzyme histochemistry ;The number of axon in the spinal white matter was surveyed in the three groups by means of the quantitative immunohitochemistry. Results (1)AchE activity of anterior horn cells in the A,B,C groups were AB >C;(2)The number of axon in a certain area of spinal white matter in the A,B,C were A >B >*** The therapeutic effects of microgene pSVPoMcat implanted to modify SC on the experimental SCI have the reliable morphological evidence.

关键词： spinal cord injury microgene enzyme histochemistry immunohistochemistry

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Effect of Hypertension on Hearing Function,LDH and ChE of the Cochlea in Older Rats

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第3期23卷 306-309页

作者：李穗龚树生杨燕珍余青松 Department of Otolaryngology

The relationship between the hypertension and the aging process of hearing organ was investigated Twenty Wistar 3-month old rats and 20 Wistar 12-month old rats, 20 spontaneously hypertensive rat stroke-prone (SHRSP) 3-month old rats and 20 SHRSP 12-month old rats free of middle ear infections as observed under otomicroscopy, with normal tympanic membrane and auricle reflex, were selected to be divided into two experimental groups and two control groups respectively The tail artery blood pressure was measured non-invasively The threshold of auditory brainstem response (ABR) was measured by Spirit TM evoked potential meter The LDH and ChE staining in the inner ear was performed and the optical density was analyzed by the HPIAS analysis system The results showed that there was no difference in the ABR thresholds, the activities of LDH and ChE between Wistar 3-month old group and SHRSP 3-month old group ( P >0 05) The mean value of ABR threshold and the activities of LDH and ChE in the Wistar 12-month old group at relevant sections were significantly greater than those in the two 3-month old groups ( P< 0 05), whereas the mean value of ABR threshold and the activities of LDH and ChE in the SHRSP 12-month old group at relevant sections were significantly higher than those in the 3-month old control group ( P< 0 01) It was concluded that presbycusis existed in the Wistar 12-month old group rats The glycogenosis and the abnormal secretion of neural transmitter were discerned after hypertension All the above factors may worsen the aging of the hearing system

关键词： spontaneously hypertensive rat stroke-prone hypertension presbycusis cochlea electric physiology enzyme histochemistry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Research on Activity and Expression of Tyrosinase in Varicorhinus macrolepis

引用

Agricultural Science & Technology 2012年第2期13卷 417-420页

作者：庞秋香王阳阳赵博生孙欢欢山东理工大学生命科学院山东淄博255049

[Objective] This study aimed to analyze tyrosinase activity and its expression in Varicorhinus macrolepis. [Method] V. macrolepis was used as experimental material for the analysis and research of tyrosinase in nine kinds of organs and tissues of male and female V. macrolepis individuals by using polyacrylamide gel electrophoresis and biochemical staining method, spectrophotometry and enzyme histochemical technology. [Result] Tyrosinase exists in the liver and pancreas, intestine and spleen of female and male V. macrolepis and in the gallbladder of male V. macrolepis. Tyrosinase activities in various tissues of V. macrolepis varied largely. Specifically, tyrosinase activities in the spleen was the maximum, which was higher in female V. macrolepis than in males. According to the enzyme histochemistry results, strong positive signals of tyrosinase existed in the spleen, intestine, liver and pancreas and gallbladder of V. macrolepis, which was the strongest in the spleen. [Conclusion] In this paper, research on tissue localization of tyrosinase in V. macrolepis had been first reported, which provided theoretical basis for further exploring the functions of tyrosinase in V. macrolepis.

关键词： V. macrolepis Tyrosinase Electrophoresis Spectrophotometry enzyme histochemistry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：