检索结果-南通市图书馆

Inducible effects of icariin,icaritin,and desmethylicaritin on directional differentiation of embryonic stem cells into cardiomyocytes in vitro

引用

Acta Pharmacologica Sinica 2005年第4期26卷 477-485页

作者： Dan-yah ZHU Yi-jia LOU~2 Department of Pharmacology and Toxicology,College of Pharmaceutical Scienees,Zhejiang University,Hangzhou 310031,China Department of Pharmacology and Toxicology College of Pharmaceutical Sciences Zhejiang University Hangzhou China

Aim:To investigate the possible inducible effects of icariin,icaritin,anddesmethylicaritin on the directional differentiation of embryonic stem(ES)cellsinto cardiomyocytes in ***:ES cells were cultivated as embryoidbodies(EBs)in hanging drops with icariin,icaritin,or *** cellstreated with retinoic acid and with solvent were used as positive and negativecontrols,*** cardiomyocytes derived from the ES cells were veri-fied using *** expression of cardiac developmental-dependent genes was detected using the reverse transcription-polymerase chainreaction(RT-PCR)*** cycle distribution and apoptosis were analyzedusing flow cytometry to determine the partly inducible effect mechanisms ***:The total percentage of beating EBs treated with 1×10-7mol/L icariin,icaritin,or desmethylicaritin was 87%(Pcells expressed cardiac-specific proteins for α-actinin and troponin *** them,1×10-7mol/L icariintreatment resulted in a significantly advanced and increased mRNA level ofα-cardiac myosin heavy chain(MHC)and myosin light chain 2v(MLC-2v)in EBsin the early cardiac developmental *** shifting to the cardiomyocytephenotype,icariin could evoke the accumulation of ES cells in G0/G1and acceler-ate apoptosis of the cell population(Pcells into cardiomyocytes at a concentration of1×10-7mol/*** promoting effect of icariin on cardiac differentiation was relatedto increasing and accelerating gene expression of a-cardiac MHC and MLC-2v,aswell as regulating the cell cycles and inducing apoptosis.

关键词： embryonic stein cells directional differentiation cardiomyocytes icariin icaritin desmethylicaritin myosin heavy chain myosin light chain 2 cell cycle apoptosis

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：