Inducible effects of icariin,icaritin,and desmethylicaritin on directional differentiation of embryonic stem cells into cardiomyocytes in vitro
Inducible effects of icariin,icaritin,and desmethylicaritin on directional differentiation of embryonic stem cells into cardiomyocytes in vitro作者机构：Department of Pharmacology and Toxicology College of Pharmaceutical Sciences Zhejiang University Hangzhou China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2005年第26卷第4期
页 面：477-485页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：国家自然科学基金 国家教育部重点项目 浙江省国际合作重点项目
主 题：embryonic stein cells directional differentiation cardiomyocytes icariin icaritin desmethylicaritin myosin heavy chain myosin light chain 2 cell cycle apoptosis
摘 要：Aim:To investigate the possible inducible effects of icariin,icaritin,anddesmethylicaritin on the directional differentiation of embryonic stem(ES)cellsinto cardiomyocytes in ***:ES cells were cultivated as embryoidbodies(EBs)in hanging drops with icariin,icaritin,or *** cellstreated with retinoic acid and with solvent were used as positive and negativecontrols,*** cardiomyocytes derived from the ES cells were veri-fied using *** expression of cardiac developmental-dependent genes was detected using the reverse transcription-polymerase chainreaction(RT-PCR)*** cycle distribution and apoptosis were analyzedusing flow cytometry to determine the partly inducible effect mechanisms ***:The total percentage of beating EBs treated with 1×10-7mol/L icariin,icaritin,or desmethylicaritin was 87%(P0.01),59%(P0.01),and 49%,*** the beating cardiomyocytes derived from the ES cells expressed cardiac-specific proteins for α-actinin and troponin *** them,1×10-7mol/L icariintreatment resulted in a significantly advanced and increased mRNA level ofα-cardiac myosin heavy chain(MHC)and myosin light chain 2v(MLC-2v)in EBsin the early cardiac developmental *** shifting to the cardiomyocytephenotype,icariin could evoke the accumulation of ES cells in G0/G1and acceler-ate apoptosis of the cell population(P0.05).Conclusion:Icariin facilitated thedirectional differentiation of ES cells into cardiomyocytes at a concentration of1×10-7mol/*** promoting effect of icariin on cardiac differentiation was relatedto increasing and accelerating gene expression of a-cardiac MHC and MLC-2v,aswell as regulating the cell cycles and inducing apoptosis.
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