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Pathogen-informed breeding for crop disease resistance

Journal of Integrative Plant Biology 2021年第2期63卷 305-311页

作者： Qi Li Bi Wang Jinping Yu Daolong Dou Jiangsu Key Laboratory for the Research and Utilization of Plant Resources Institute of BotanyJiangsu Province and Chinese Academy of SciencesNanjing 210014China Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095China

The development of durable and broad-spectrum resistance is an economical and eco-friendly approach to control crop diseases for sustainable agricultural production. Emerging knowledge of the molecular basis of pathogenesis and plant–pathogen interactions has contributed to the development of novel pathogen-informed breeding strategies beyond the limits imposed by conventional breeding. Here,we review the current status of pathogen-assisted resistance-related gene cloning. We also describe how pathogen effector proteins can be used to identify resistance resources and to inform cultivar deployment. Finally, we summarize the main approaches for pathogen-directed plant improvement,including transgenesis and genome editing. Thus, we describe the emerging role of pathogen-related studies in the breeding of disease-resistant varieties, and propose innovative pathogen-informed strategies for future applications.

关键词： disease resistance breeding effector protein plant immune system plant pathogens

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Two NIS1-like proteins from apple canker pathogen(Valsa mali)play distinct roles in plant recognition and pathogen virulence

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Stress Biology 2022年第1期2卷 606-620页

作者： Jiajun Nie Wenjing Zhou Yonghui Lin Zhaoyang Liu Zhiyuan Yin Lili Huang State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University3 Taicheng RoadYangling 712100ShaanxiChina College of Plant Protection China Agricultural UniversityBeijing 100193China

Conserved effectors produced by phytopathogens play critical roles in plant-microbe ***1-like proteins represent a newly identified family of effectors distributed in multiple fungal ***,their biological functions in a majority of pathogenic fungi remain largely elusive and require further *** this study,we characterized two NIS1-like proteins VmNIS1 and VmNIS2 from Valsa mali,the causal agent of apple Valsa *** of these two proteins were predicted to be *** agroinfiltration,we found that VmNIS1 induced intense cell death,whereas VmNIS2 suppressed INF1 elicitin-triggered cell death in Nicotiana *** of *** with VmNIS1 recombinant protein produced by Escherichia coli activated a series of immune responses and enhanced plant disease resistance against Phytophthora *** contrast,VmNIS2 suppressed plant immune responses and promoted *** infection when transiently expressed in *** VmNIS1 and VmNIS2 were shown to be highly induced at late stage of *** *** individually knocking out of these two genes in ***,however,only VmNIS2 was shown to be required for pathogen virulence as well as tolerance to oxidative ***,we further showed that C-terminal extension of VmNIS1 was essential for plant recognition and VmNIS2 may escape plant detection via sequence *** data collectively indicate that VmNIS1 and VmNIS2 play distinct roles in plant recognition and pathogen virulence,which provided new insights into the function of NIS1-like proteins in plant-microbe interactions.

关键词： Valsa mali effector protein Cell death Plant immunity Virulence

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Functional Characterization of a Novel Chloroplast-targeting Non-classically Secreted protein(PcCt2) in the Pathogenesis of Phytophthora capsici

Functional Characterization of a Novel Chloroplast-targeting...

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中国植物病理学会2024年学术年会

作者： HUA Cai LAI Bingting QIU Xiang GUO Yaru XU Kaiyi LI Linying ZHANG Kaidi GUO Hengyuan LIU Wenbo Justice Norvienyeku Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests Ministry of EducationSchool of Tropical Agriculture and ForestryHainan University Danzhou Invasive Species Observation and Research Station of Hainan Province Hainan University

The oomycetes Phytophthora capsici is an economically destructive plant pathogen that causes Phytophthora-blight disease in multiple fruit and vegetable crops,including Capsicum annum,Solanum lycopersicum,Citrullus lanatus,Vicia faba,Solanum melongena among *** Phytophthora-blight pathogen remains a significant threat to global food security due to inadequate knowledge of the molecular determinants of pathogenicity or virulence of *** during pathogen-host interaction to support the development of sustainable and environmentally friendly disease control *** and effectome analysis revealed a hypothetical protein containing chloroplast-targeting peptide(cTP) in ***(PcCt2) as a novel non-classically secreted candidate effector protein that targets host chloroplast during pathogen-host ***,we deployed transcriptomic,biochemical,and functional geneticstrategies,including CRISPR-Cas9 gene editing and homologous recombination approach to generate PcCT2 targeted gene replacement *** exclusive and significant up-regulation in the expression pattern of PcCT2 at early and late stages of *** interaction with pepper,tomato,faba bean,kidney bean,watermelon,and *** the same time,phenotypic characterization of the ΔPcct2 strains showed that targeted replacement of PcCT2 triggered a significant reduction in the vegetative growth,sporulation,stress tolerance,and virulence of ***-fold2-based pairwise screening of pepper proteome with PcCT2 identified 16 chloroplast localized putative interaction *** from Yeast-two-hybrid analysis confirmed a physical-geneticinteraction between PcCT2 and chloroplast localized Ethylene-responsive transcription *** findings underscore the significance of non-classically secreted effector proteins in the pathogenesis of *** and also projected PcCT2 as a potential target for developing oomyticides against the Phytophthora-blight pa

关键词： Phytophthora capsici effector protein Pathogen-host interaction Microbial Pathogenesis Non-classically secreted protein

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Transcription profile analysis of interaction mechanism between sugarcane and Sporisorium scitamineum using microarray

Transcription profile analysis of interaction mechanism betw...

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2019年中国作物学会学术年会

作者：黄宁 Hui Ling Weihua Su Yachun Su Youxiong Que Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian) Ministry of Agriculture Fujian Agriculture and Forestry University Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization Ministry of Education Fujian Agriculture and Forestry University

【Research background】 Sugarcane production is seriously affected by Sporisorium scitamineum infection in the world. Although some excellent resistant breeding materials have been created, the utilization of sugarcane germplasm and the breeding of resistant varieties are still greatly restricted for the complex genetic background of sugarcane and the differentiation of smut race. To solve this problem, much effort should be devoted to underlying the mechanism of sugarcane smut resistance and S. scitamineum virulence. 【Materials and methods】 According to our previous RNA-seq and smut pathogen genome data, the gene expression chip was customized. The transcription profile of gene set, which referred to 2,0392 from sugarcane and the whole CDS sequence(6,621) from S. scitamineum genome, was identified on smut spore injected samples. These samples were from sugarcane smut-susceptible genotype YC71-374 and smut-resistant genotype NCo376. 【Result and analysis】 During the interaction between sugarcane and smut fungus, 6,842 sugarcane genes and 3,569 S. scitamineum genes were differentially expressed. According to the GO and KEGG enrichment analysis, the differential expressed sugarcane genes involved in primary metabolism pathway, MAPK pathway, plant hormone signal transduction pathway and ROS response, the differential expressed S. scitamineum genes mostly located in vesicles, and have activities like transportation, signal transduction and redox. After that, the differential expressed genes from sugarcane and S. scitamineum were selected and used to construct the weighted gene co-expression(WGCNA) network of sugarcane-S. scitamineum interaction. Finally, two sugarcane smut-resistance related genes(vacuolar-sorting receptor, ScVSR;and leucine-rich repeat receptor-like protein kinase, Sc LRR-RLK) and four putative S. scitamineum effector genes, including protein essential during penetration 1(SsPep1), hypothetical cysteine-protease inhibitor(SsE4), conserved hypothetical pro

关键词： sugarcane Sporisorium scitamineum microarray WGCNA resistance gene effector protein

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Genomic Evolution and Diversity in Botryosphaeriales:Insights from Pan-Genomic and Population Genetic Analyses of Representative Species

Genomic Evolution and Diversity in Botryosphaeriales:Insight...

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中国菌物学会2024年学术年会

作者： Zong-Lin Deng Yong Zhang Jian-Kui Liu School of Life Science and Technology Center for Informational BiologyUniversity of Electronic Science and Technology of China

Botryosphaeriales is an ecologically significant fungal order within the class Dothideomycetes of the phylum Ascomycota,exerting substantial economic and ecological *** order comprises hundreds of known species across 32 genera and six families,interacting with plant hosts through endophytic,saprophytic and pathogenic fungal *** its significance,the genomic landscape of Botryosphaeriales remains largely *** this study,we selected three representative species,Botryosphaeria dothidea,Neofusicoccum parvum,and Phyllosticta capitalensis,and de novo assembled reference-quality *** integrating available genome-wide data,we conducted a comprehensive pan-genomic analysis coupled with population-based genetic evolutionary *** approach allowed us to investigate genomic evolution dynamics among different strains within the same species,genomic divergences between species within the same genus,and the evolutionary relationships across various *** finding revealed a significant expansion in families of genes related to membrane transport in pathogenic fungi compared to *** pan-genomic and genetic evolutionary analyses indicated that Botryosphaeria dothidea exhibits higher genomic diversity than Neofusicoccum parvum,suggesting that the formation of distinct subgroups within the species may result from varying selective *** the genus level,the expansion of specific gene families and horizontal genome transfer were identified as key drivers of genome diversity and evolution among ***,we observed a high degree of synteny between species within different families of *** number of cytoplasmic effectors in various species was found to be approximately ten times that of apoplectic ***,the phylogenetic tree topology of putative effector proteins did not align with the results of species-wide genomic phylogenetic trees,indicating potential inconsistencies i

关键词： Botryosphaeriales pan-genomic population genetic evolution selective sweep comparative genomes effector protein

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