Functional Characterization of a Novel Chloroplast-targeting Non-classically Secreted Protein(PcCt2) in the Pathogenesis of Phytophthora capsici
作者单位：Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and PestsMinistry of EducationSchool of Tropical Agriculture and ForestryHainan University Danzhou Invasive Species Observation and Research Station of Hainan ProvinceHainan University
会议名称：《中国植物病理学会2024年学术年会》
会议日期：2024年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
关 键 词：Phytophthora capsici effector protein Pathogen-host interaction Microbial Pathogenesis Non-classically secreted protein
摘 要：The oomycetes Phytophthora capsici is an economically destructive plant pathogen that causes Phytophthora-blight disease in multiple fruit and vegetable crops,including Capsicum annum,Solanum lycopersicum,Citrullus lanatus,Vicia faba,Solanum melongena among *** Phytophthora-blight pathogen remains a significant threat to global food security due to inadequate knowledge of the molecular determinants of pathogenicity or virulence of *** during pathogen-host interaction to support the development of sustainable and environmentally friendly disease control *** and effectome analysis revealed a hypothetical protein containing chloroplast-targeting peptide(cTP) in ***(PcCt2) as a novel non-classically secreted candidate effector protein that targets host chloroplast during pathogen-host ***,we deployed transcriptomic,biochemical,and functional geneticstrategies,including CRISPR-Cas9 gene editing and homologous recombination approach to generate PcCT2 targeted gene replacement *** exclusive and significant up-regulation in the expression pattern of PcCT2 at early and late stages of *** interaction with pepper,tomato,faba bean,kidney bean,watermelon,and *** the same time,phenotypic characterization of the ΔPcct2 strains showed that targeted replacement of PcCT2 triggered a significant reduction in the vegetative growth,sporulation,stress tolerance,and virulence of ***-fold2-based pairwise screening of pepper proteome with PcCT2 identified 16 chloroplast localized putative interaction *** from Yeast-two-hybrid analysis confirmed a physical-geneticinteraction between PcCT2 and chloroplast localized Ethylene-responsive transcription *** findings underscore the significance of non-classically secreted effector proteins in the pathogenesis of *** and also projected PcCT2 as a potential target for developing oomyticides against the Phytophthora-blight pa