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Traditional Medicine Research 2024年第3期9卷 24-31页

作者： Si Qin Jing-Lou Chen Xiao-Feng Zhou Cong-Yue Xu Jing Guo Department of Pharmacy Jianghan UniversityWuhan 430056China Department of Clinical Medicine Jianghan UniversityWuhan 430056China Institutes of Biomedical Sciences Jianghan UniversityWuhan 430056China Department of Basic Medicine School of MedicineJianghan UniversityWuhan 430056China

Background:Lower urinary tract symptoms commonly occur in the elderly population and seriously constrain the quality of life.Glandular fibrosis is an important pathobiological process in benign prostatic hyperplasia and is also a main inducing factor for benign prostatic hyperplasia-associated lower urinary tract symptoms.Cistanches species is an important herbal medicine resource and is traditionally used in ameliorating renal and prostatic defects.Methods:This study was to investigate the potential protective function of echinacoside(a bioactive compound from Cistanches)against prostatic fibrosis in mice and human benign prostatic hyperplasia epithelial-1 cell models.Results:It was found that echinacoside attenuated testosterone-induced prostatic hyperplasia and collagen deposition in mice,relieved prostate local inflammation and oxidative damage,and ameliorated prostatic epithelial-mesenchymal transition.Additionally,echinacoside inhibited the activation of the MKK6/MK2 signaling pathway both in vivo and in vitro.Conclusion:This study added new evidence for the anti-fibrotic function of echinacoside on the prostate and provided new insights for understanding its possible pharmacological mechanisms.

关键词： benign prostatic hyperplasia echinacoside epithelial-mesenchymal transition fibrosis

echinacoside Alleviates Cognitive Impairment in Cerebral Ischemia Rats through α7nAChR-Induced Autophagy

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Chinese Journal of Integrative Medicine 2022年第9期28卷 809-816页

作者： DING Ling YE Hong GU Long-dian DU An-qing YUAN Xin-lu Department of Endocrinology and Metabolic Diseases Shanghai Pudong HospitalFudan University Pudong Medical CenterShanghai201399China

Objectives:To evaluate the effect of echinacoside(ECH)on cognitive dysfunction in post cerebral stroke model rats.Methods:The post stroke cognitive impairment rat model was created by occlusion of the transient middle cerebral artery(MCAO).The rats were randomly divided into 3 groups by a random number table:the sham group(sham operation),the MCAO group(received operation for focal cerebral ischemia),and the ECH group(received operation for focal cerebral ischemia and ECH50 mg/kg per day),with 6 rats in each group,The infarct volume and spatial learning were evaluated by triphenyl tetrazolium chloride staining and Morris water maze.The expression of α7 nAChR in the hippocampus was detected by immunohistochemistry.The contents of acetylcholine(ACh),malondialdehyde(MDA),glutathione(GSH),superoxide dismutase(SOD),activities of choline acetyltransferase(ChAT),acetylcholinesterase(AChE),and catalase(CAT)were evaluated by enzyme linked immunosorbent assay.The neural apoptosis and autophagy were determined by TUNEL staining and LC3 staining,respectively.Results:ECH significantly lessened the brain infarct volume and ameliorated neurological deficit in infarct volume and water content(both P<0.01).Compared with MCAO rats,administration of ECH revealed shorter escape latency and long retention time at 7,14 and 28 days(all P<0.01),increased the α7 nAChR protein expression,ACh content,and ChAT activity,and decreased AChE activity in MCAO rats(all P<0.01).ECH significantly decreased MDA content and increased the GSH content,SOD,and CAT activities compared with MCAO rats(all P<0.05).ECH suppressed neuronal apoptosis by reducing TUNEL-positive cells and also enhanced autophagy in MCAO rats(all P<0.01).Conclusion:ECH treatment helped improve cognitive impairment by attenuating neurological damage and enhancing autophagy in MCAO rats.

关键词： cerebral ischemia cognitive impairment autophagy echinacoside cholinergic activity

echinacoside Protects Against MPP+-Induced Neuronal Apoptosis via ROS/ATF3/CHOP Pathway Regulation

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Neuroscience Bulletin 2016年第4期32卷 349-362页

作者： Qing Zhao Xiaoyan Yang Dingfang Cai Ling Ye Yuqing Hou Lijun Zhang Jiwei Cheng Yuan Shen Kaizhe Wang Yu Bai Department of Neurology Putuo HospitalShanghai University of Traditional Chinese Medicine Department of Emergency Internal Medicine Putuo HospitalShanghai University of Traditional Chinese Medicine Laboratory of Neurology Institute of Integrative MedicineZhongshan Hospital Fudan University Department of Integrative Medicine Zhongshan HospitalFudan University Center for Translational Neurodegeneration and Regenerative Therapy Shanghai Tenth People’s Hospital Affiliated with Tongji University School of Medicine Department of Immunology Tongji University School of Medicine

echinacoside （ECH） is protective in a mouse model of Parkinson＇ s disease （PD） induced by 1-methyl-4- phenylpyridinium ion （MPP＋）. To investigate the mechanisms involved, SH-SYSY neuroblastoma ceils were treated with MPP＋ or a combination of MPP＋ and ECH, and the expression of ATF3 （activating transcription factor 3）, CHOP （C/EBP-homologous protein）, SCNA （synuclein alpha）, and GDNF （glial cell line-derived neurotrophic factor） was assessed. The results showed that ECH significantly improved cell survival by inhibiting the generation of MPP＋-induced reactive oxygen species （ROS）. In addition, ECH suppressed the ROS and MPP＋- induced expression of apoptotic genes （ATF3, CHOP, and SCNA）. ECH markedly decreased the MPP＋-induced cas- pase-3 activity in a dose-dependent manner. ATF3- knockdown also decreased the CHOP and cleaved caspase- 3 levels and inhibited the apoptosis induced by MPP＋. Interestingly, ECH partially restored the GDNF expression that was down-regulated by MPP＋. ECH also improved dopaminergic neuron survival during MPP＋ treatment and protected these neurons against the apoptosis induced by MPTP. Taken together, these data suggest that the ROS/ ATF3/CHOP pathway plays a critical role in mechanisms by which ECH protects against MPP＋-induced apoptosis in PD.

关键词： echinacoside Parkinson＇s disease 1-Methyl-4-phenylpyridinium ion. Reactive oxygen species ATF3 CHOP

Effects of echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats

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Biomedical and Environmental Sciences 2012年第2期25卷 238-244页

作者： WEI Li Li CHEN Hong JIANG Yong TU Peng Fei ZHONG Ming DU Juan LIU Fei WANG Lei LIU Chun Yang Ministry of Education Key Laboratory of Xinjiang Phytomedicine Resources Shihezi University Shihezi 832002 Xinjiang China Department of Pharmacology College of Pharmacy Shihezi University Shihezl 832002 Xinjiang China Peking University Modern Research Center for Traditional Chinese Medicine Beijing 100083 China

Objective To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine（NE）,dopamine（DA）,homovanillic acid（HVA）,3,4-dihydroxyphenylethanoid acid（DOPAC）,5-hydroxyindoleacetic acid（HIAA）,and 5-hydroxytryptamine（5-HT） in middle cerebral artery occlusion（MCAO rats.Methods The middle cerebral artery was occluded in male Sprague-Dawley rats.Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer＇s solution at a rate of 1.5 μL/min.Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE,DA,HVA,DOPAC,HIAA,and 5-HT levels by reverse phase HPLC with electrochemistry.Results Three days after MCAO,the extracellular striatal levels of NE,DA,DOPAC,HIAA,HVA,and 5-HT of the MCAO rats increased significantly（at least P0.05 vs.control）.However,simultaneous treatment with echinacoside（30.0 or 15.0 mg/kg） attenuated these increases（at least P0.05 vs.non-treated model rats）.Conclusion These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.

关键词： echinacoside Norepinephrine （NE） dopamine （DA） homovanillic acid （HVA） 3,4-dihydroxyphenylethanoid acid （DOPAC） 5-hydroxyindoleacetic acid （HIAA） 5-hydroxytryptamine （5-HT）,Middle cerebral artery occlusion（MCAO） Brain microdialysis rats

Application of ~1H NMR-based metabolomics for discrimination of different parts and development of a new processing workflow for Cistanche deserticola

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Acta Pharmaceutica Sinica B 2017年第6期7卷 647-656,676页

作者： Pingping Zou Yuelin Song Wei Lei Jun Li Pengfei Tu Yong Jiang State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University Modern Research Center for Traditional Chinese Medicine School of Chinese Materia Medica Beijing University of Chinese Medicine

Cistanche deserticola(CD) is one of the two authoritative source plants of Cistanches Herba, a well-known medicinal plant. Herein,~1H NMR spectroscopy was employed to characterize the chemical profile and to distinguish the different parts, as well as to propose a new processing workflow for CD.Signal assignment was achieved by multiple one and two dimensional NMR spectroscopic techniques in combination with available databases and authentic compounds. The upper parts of the plant were distinguished from the lower parts by combining ~1H NMR spectroscopic dataset with multivariate statistical analysis. A new processing method that hyphenated steaming with freeze-drying, was demonstrated to be superior to either steaming coupled with oven-drying or direct freeze-drying via holistic ~1H NMR-based metabolomic characterization. Phenylethanoid glycosides, mainly echinacoside and acteoside, were screened out and confirmed as the chemical markers responsible for exhibiting the superiority of the new processing workflow, whereas serial primary metabolites, especially carbohydrates and tricarboxylic acid cycle metabolites, were found as the primary molecules governing the discrimination between the upper and lower parts of the plant. Collectively,~1H NMR spectroscopy was demonstrated as a versatile analytical tool to characterize the chemical profile and to guide the indepth exploitation of CD by providing comprehensive qualitative and quantitative information.

关键词： Cistanche deserticola 1H NMR-based metabolomics Processing workflow Different parts Phenylethanoid glycoside Tricarboxylic acid cycle metabolites echinacoside Acteoside

echinacoside attenuates 6-hydroxydopamine-induced neurotoxicity in PC12 cells

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Echinacoside attenuates 6-hydroxydopamine-induced neurotoxic...

Content Determination of Cistanches Herba in Zhenrongdan Mixture by HPLC

第二届世界天然药物和传统药物药理学学术会议

作者： Yue-Hua WANG~(1,2),Shuo TIAN~2,Guo-Rong HE~2,Lian-Hua FANG~2,Xiao-Ping PU~(1*),Guan-Hua DU~(2*) (1.Department of Molecular and Cellular Pharmacology,School of Pharmaceutical Sciences,Peking University, Beijing 100191,China 2.Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050,China)

＜正＞AIM To investigate the effects and the underlying mechanism of echinacoside（ECH） against 6-OHDA-induced injury in vitro.METHODS 6-OHDA was added to cultured PC12 cells after pretreatment for 1 h with different concentration of ECH or saline and co-incubated for another 24 h.To evaluate the protective effects of ECH,the morphological change was observed by microscope,the viability of PC12 cells was assayed by MTT method.To explore the mechanism of ECH against 6-OHDA neurotoxicity, the mitochondrial oxidation-reduction activity（REDOX）,mitochondrial membrane potential（△ψm）,reactive oxygen species（ROS） production,ATP level,cytochrome c release,the activity of caspase 3,and apoptosis in PC12 cells were measured.RESULTS 6-OHDA induced the decrease of cell viability,ROS generation increase,ATP content decrease,mitochondrial dysfunction,and mitochondria -mediated apoptosis in PC12 cells.ECH could attenuate 6-OHDA-induced neurotoxicity,inhibit the collapse of△ψm,reduce the mitochondrial dysfunction.ECH also inhibited cyt c release and caspase 3 activation.CONCLUSION Our results indicate that ECH attenuates 6-OHDA-induced neurotoxicity through anti-oxidative and anti-apoptosis mediated by mitochondria.

关键词： echinacoside Parkinson’s disease 6-hydroxydopamine

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Medicinal Plant 2022年第5期13卷 4-7页

作者： Jianping ZHANG Xuemei ZHOU Han GAO Dong WANG Inner Mongolia Autonomous Region Institute for Drug Control Huhhot 010020China

[Objectives]To establish a HPLC-UV method for the determination of Cistanches Herba in Zhenrongdan mixture.[Methods]According to the content determination method of Cistanches Herba in the 2020 edition of Chinese Pharmacopoeia,using HPLC-UV method,the content determination method of Cistanches Herba in Zhenrongdan mixture was established,and the content of echinacoside was determined.The related chromatographic conditions were explored,and the methodological investigation was carried out.[Results]The chromatographic conditions were determined as follows:octadecylsilane bonded silica gel as filler(C_(18));80%acetonitrile solution(containing 0.2%phosphoric acid)-0.2%phosphoric acid aqueous solution(15∶85)as mobile phase;detection wavelength was 330 nm.The number of theoretical plates should not be less than 3000 according to the peak of echinacoside.The methodological investigation showed that the method had better precision,accuracy and repeatability.Under the conditions of this study,echinacoside had better linear relationship in the range of 74.9-1498 ng,and the average recovery was 99.1%,RSD=0.5%(n=6).[Conclusions]A method for the determination of Cistanches Herba in Zhenrongdan mixture by HPLC-UV was established.The method is sensitive,rapid,accurate and suitable for the determination of Cistanches Herba in Zhenrongdan mixture.

关键词： HPLC-UV Zhenrongdan mixture Cistanches Herba echinacoside

Complementary Application of HSCCC and Semi-preparative HPLC for Rapid Separation of Phenylethanoid Glycosides from Penstemon digitalis

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Chinese Herbal Medicines 2013年第4期5卷 280-285页

作者： WANG Qi-yan XIE Jun FANG Sheng SHEN Xiu-lian LI Qi WU Xiu-hua TANG Shao-hu Department of Chemical Biology School of Chemistry and Chemical Engineering Anhui University of Technology School of Life Sciences Southwest University Department of Applied Chemistry School of Chemistry and Chemical Engineering Anhui University of Technology

Objective echinacoside and verbascoside are known for their excellent neuroprotective,anti-inflammatory,and anti-oxidative activities,therefore large amount of pure compounds are urgently needed as authentic standards for various in vivo and in vitro studies.Nowadays,they are abundenty extracted from endangered Cistanche spp.Phytochemical studies revealed that Penstemon spp.,comprising about 280 species,were rich natural sources for the exploitation and large scale preparation of phenylethanoid glycosides.Thus,rapid isolation and purification of various phenylethanoid glycosides from Penstemon spp.were necessary.Methods The crude extract of P.digitalis was first enriched by AB-8,and then the high-speed counter-current chromatography(HSCCC)combined with semipreparative high performance liquid chromatography(HPLC)method was adopted to isolate and purify echinacoside and verbascoside.Results Eventually,verbascoside(67.2 mg)with the purity of 92.6%and echinacoside(3.96 mg)with the purity of 98.9%were obtained from 5 g powdered leaves of P.digitalis.Conclusion The present mode of HSCCC coupled with semi-preparative HPLC could be a powerful method for the rapid isolation and purification of other phenylethanoid glycosides.

关键词： echinacoside high-speed counter-current chromatography Penstemon digitalis semi-preparative high performance liquid chromatography verbascoside

Prevention of Cistanche salsa Extract on Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

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Chinese Herbal Medicines 2013年第3期5卷 199-204页

作者： YANG Feng-rui WEN Du-su FANG Bu-wu LOU Jian-shi MENG Lin Department of Pharmacology Tianjin Medical University

Objective To explore the antifibrotic effect of echinacoside on carbon tetrachloride(CCl4)-induced hepatic fibrosis in rats.Methods Male Wistar rats were randomly divided into normal control(n = 8),model(n = 14),and echinacoside treatment(n = 14) groups.The hepatic fibrosis model was induced by CCl4compositor.The rats were ig administered with echinacoside at a daily dose of 50 mg/kg.The anti-oxidant status,liver function parameters,and hepatic hydroxyproline content were detected by chromatometry.The serum levels of hyaluronic acid(HA),type IV collagen(CIV),type III precollagen(PIIIP),and laminin(LN) were assayed with radioimmunoassay.The hpatic injury was detected by haematoxylin-eosine staining.The deposition of collagen was observed with Masson staining.Results echinacoside increased the superoxide dismutase activity and reduced the levels of malondialdehyde,aspartate aminotransferase,alanine aminotransferase,HA,CIV,PIIIP,and LN in serum.echinacoside could also reduce the hydroxyproline content in liver,alleviate hepatic injury,and inhibit collagen deposition.Conclusion echinacoside possesses antihepatic fibrosis effect.

关键词： carbon tetrachloride echinacoside extracellular matrix hepatic fibrosis lipid peroxidation

echinacoside中译名的商榷及其研究进展

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现代药物与临床 1999年第3期14卷 130-130页

作者：陈笔岫江纪武

echinacoside的中译名较早称海胆甙(《英汉医学词汇》,1979年)或海胆苷(《英汉化学化工词汇》第3版,1984年)。以后为多种文献辞书沿用,如1987年出版的《英汉科技辞天》和《英汉科技词库》,直至现在仍有人译为海胆苷。本人认为该中译名... 详细信息

echinacoside的中译名较早称海胆甙(《英汉医学词汇》,1979年)或海胆苷(《英汉化学化工词汇》第3版,1984年)。以后为多种文献辞书沿用,如1987年出版的《英汉科技辞天》和《英汉科技词库》,直至现在仍有人译为海胆苷。本人认为该中译名值得商榷。该化合物系Stoll等人于1950年首次从菊科紫松果菊属植物狭叶松果菊(或狭叶紫锥菊)Echi-

关键词：中译名松果菊海胆英汉植物 echinacoside 紫锥菊