Echinacoside Alleviates Cognitive Impairment in Cerebral Ischemia Rats through α7nAChR-Induced Autophagy

作     者：DING Ling YE Hong GU Long-dian DU An-qing YUAN Xin-lu DING Ling;YE Hong;GU Long-dian;DU An-qing;YUAN Xin-lu

作者机构：Department of Endocrinology and Metabolic DiseasesShanghai Pudong HospitalFudan University Pudong Medical CenterShanghai201399China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2022年第28卷第9期

页      面：809-816页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Talents Training Program of Pudong Hospital Affiliated to Fudan University(No.PX202007) 

主　　题：cerebral ischemia cognitive impairment autophagy echinacoside cholinergic activity 

摘      要：Objectives:To evaluate the effect of echinacoside(ECH)on cognitive dysfunction in post cerebral stroke model ***:The post stroke cognitive impairment rat model was created by occlusion of the transient middle cerebral artery(MCAO).The rats were randomly divided into 3 groups by a random number table:the sham group(sham operation),the MCAO group(received operation for focal cerebral ischemia),and the ECH group(received operation for focal cerebral ischemia and ECH50 mg/kg per day),with 6 rats in each group,The infarct volume and spatial learning were evaluated by triphenyl tetrazolium chloride staining and Morris water *** expression of α7 nAChR in the hippocampus was detected by *** contents of acetylcholine(ACh),malondialdehyde(MDA),glutathione(GSH),superoxide dismutase(SOD),activities of choline acetyltransferase(ChAT),acetylcholinesterase(AChE),and catalase(CAT)were evaluated by enzyme linked immunosorbent *** neural apoptosis and autophagy were determined by TUNEL staining and LC3 staining,***:ECH significantly lessened the brain infarct volume and ameliorated neurological deficit in infarct volume and water content(both P0.01).Compared with MCAO rats,administration of ECH revealed shorter escape latency and long retention time at 7,14 and 28 days(all P0.01),increased the α7 nAChR protein expression,ACh content,and ChAT activity,and decreased AChE activity in MCAO rats(all P0.01).ECH significantly decreased MDA content and increased the GSH content,SOD,and CAT activities compared with MCAO rats(all P0.05).ECH suppressed neuronal apoptosis by reducing TUNEL-positive cells and also enhanced autophagy in MCAO rats(all P0.01).Conclusion:ECH treatment helped improve cognitive impairment by attenuating neurological damage and enhancing autophagy in MCAO rats.