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Aggregation-induced emission luminogen-assisted stimulated emission depletion nanoscopy for super-resolution mitochondrial visualization in live cells

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Nano Research 2018年第11期11卷 6023-6033页

作者： Dongyu Li Xiang Ni Xiaoyan Zhang Liwei Liu Junle Qu Dan Ding Jun Qian State Key Laboratory of Modern Optical Instrumentation Centre for Optical and Electromagnetic Research Zhejiang Provincial Key Laboratory for Sensing Technologies Zhejiang University Hangzhou 310058 China State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Bioactive Materials Ministry of Education and College of Life Sciences Nankai University Tianjin 300071 China Key Laboratory of Optoelectrical Devices and Systems Ministry of Education Shenzhen University Shenzhen 518060 China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer Institute Xuzhou Medical University Xuzhou 221004 China

Aggregation-induced emission luminogens (AIEgens) are fluorescent agents that are ideal for bioimaging and have been widely used for organelle targeting, cellular mapping, and tracing. Owing to their promising characteristics, AIEgen-based nanoparticles have recently been used for the stimulated emission depletion (STED) super-resolution imaging of fixed cells. In the present study, and for the first time, we used an AIEgen for dynamic STED nanoscopic imaging of a specific organelle in live cancer cells. TPA-T-CyP is a synthetic red&NIR-emitting luminogen with AIE features that can spontaneously and specifically aggregate on mitochondria without the need for encapsulation or surface modification. The STED efficiency of aggregated TPA-T-CyP can reach more than 80%, and super-resolution imaging of TPA-T-CyP-stained mitochondria in live HeLa cells is possible, with a lateral spatial resolution of 74 nm. We found that TPA-T-CyP enabled the dynamic visualization of mitochondria, and the motion, fusion, and fission of mitochondria were clearly observable on a super-resolution scale. AIEgen-based super-resolution organelle visualization has great potential for many basic biomedical studies.

关键词： aggregation-induced emission stimulated emission depletion nanoscopy mitochondria dynamic visualization

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A Survey of Time Series Data visualization Methods

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Journal of Quantum Computing 2020年第2期2卷 105-117页

作者： Wangdong Jiang Jie Wu Guang Sun Yuxin Ouyang Jing Li Shuang Zhou School of Information Management and Technology Hunan University of Finance and EconomicsChangsha410000China The University of Alabama TuscaloosaAlabamaUSA Housheng School of International Education Hunan University of Finance and EconomicsChangsha410000China

In the era of big data,the general public is more likely to access big data,but they wouldn’t like to analyze the ***,the traditional data visualization with certain professionalism is not easy to be accepted by the general public living in the fast *** this background,a new general visualization method for dynamic time series data emerges as the times *** series data visualization organizes abstract and hard-to-understand data into a form that is easily understood by the *** method integrates data visualization into short videos,which is more in line with the way people get information in modern fast-paced *** modular approach also facilitates public participation in *** paper summarizes the dynamic visualization methods of time series data ranking,studies the relevant literature,shows its value and existing problems,and gives corresponding suggestions and future research prospects.

关键词： dynamic visualization historical ranking of time series data video big data

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