Aggregation-induced emission luminogen-assisted stimulated emission depletion nanoscopy for super-resolution mitochondrial visualization in live cells

作     者：Dongyu Li Xiang Ni Xiaoyan Zhang Liwei Liu Junle Qu Dan Ding Jun Qian 

作者机构：State Key Laboratory of Modern Optical Instrumentation Centre for Optical and Electromagnetic Research Zhejiang Provincial Key Laboratory for Sensing Technologies Zhejiang University Hangzhou 310058 China State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Bioactive Materials Ministry of Education and College of Life Sciences Nankai University Tianjin 300071 China Key Laboratory of Optoelectrical Devices and Systems Ministry of Education Shenzhen University Shenzhen 518060 China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer Institute Xuzhou Medical University Xuzhou 221004 China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2018年第11卷第11期

页      面：6023-6033页

核心收录：

学科分类：07[理学] 

基　　金：This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LR17F050001)  the National Natural Science Foundation of China (Nos. 61735016 and 51622305)  and the National Basic Research Program of China (Nos. 2013CB834704 and 2015CB856503)  the PCSIRT (No. IRT13023)  the Science & Technology Project of Tianjin of China (No. 15JCYBJC29800)  the China Postdoctoral Science Foundation (No. 2016M601252)  and the 111 project (B08011) 

主　　题：aggregation-induced emission stimulated emission depletion nanoscopy mitochondria dynamic visualization 

摘      要：Aggregation-induced emission luminogens (AIEgens) are fluorescent agents that are ideal for bioimaging and have been widely used for organelle targeting, cellular mapping, and tracing. Owing to their promising characteristics, AIEgen-based nanoparticles have recently been used for the stimulated emission depletion (STED) super-resolution imaging of fixed cells. In the present study, and for the first time, we used an AIEgen for dynamic STED nanoscopic imaging of a specific organelle in live cancer cells. TPA-T-CyP is a synthetic red&NIR-emitting luminogen with AIE features that can spontaneously and specifically aggregate on mitochondria without the need for encapsulation or surface modification. The STED efficiency of aggregated TPA-T-CyP can reach more than 80%, and super-resolution imaging of TPA-T-CyP-stained mitochondria in live HeLa cells is possible, with a lateral spatial resolution of 74 nm. We found that TPA-T-CyP enabled the dynamic visualization of mitochondria, and the motion, fusion, and fission of mitochondria were clearly observable on a super-resolution scale. AIEgen-based super-resolution organelle visualization has great potential for many basic biomedical studies.