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Fringe optimization for structured illumination super-resolution microscope with digital micromirror device

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Journal of Innovative Optical Health Sciences 2019年第3期12卷 78-86页

作者： Xibin Yang Qian Zhu Zhenglong Sun Gang Wen Xin Jin Linbo Wang Jialin Liu Daxi Xiong Hui Li Jiangsu Key Lab of Medical Optics Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences No.88 Keling Street Suzhou 215163P.R.China University of Chinese Academy of Sciences Beijing 100049 P.R.China

Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling *** illumination can be obtained by either laser interference or projection of fringe ***,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM *** effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were *** the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil *** imaging and dynamics of subcellular organelles in live cells were also *** method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.

关键词： Structured illumination super-resolution digital micromirror device fringe pattern modulation depth

High-speed playback of spatiotemporal division multiplexing holographic 3D video stored in a solid-state drive using a digital micromirror device

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Chinese Optics Letters 2021年第9期19卷 121-126页

作者： Kohei Suzuki Minori Tao Yuki Maeda Hirotaka Nakayama Ren Noguchi Minoru Oikawa Yuichiro Mori Takashi Kakue Tomoyoshi Shimobaba Tomoyoshi Ito Naoki Takada Graduate School of Integrated Arts and Sciences Kochi UniversityKochi 780-8520Japan Faculty of Science Kochi UniversityKochi 780-8520Japan National Astronomical Observatory of Japan Tokyo 181-8588Japan Research and Education Faculty Kochi UniversityKochi 780-8520Japan Graduate School of Engineering Chiba UniversityChiba 263-8522Japan

We propose a high-speed playback method for the spatiotemporal division multiplexing electroholographic three-dimensional(3D)video stored in a solid-state drive(SSD)using a digital micromirror *** spatiotemporal division multiplexing electroholography prevents deterioration in the reconstructed 3D video from a 3D object comprising many object *** the proposed method,the stored data is remarkably reduced using the packing technique,and the computer-generated holograms are played back at high ***,we successfully reconstructed a clear 3D video of a 3D object comprising approximately 1,100,000 points at 60 frames per second by reducing the reading time of the stored data from an SSD.

关键词： spatiotemporal division multiplexing electroholography digital micromirror device computer-generated hologram high-speed playback

A Compressive Sensing Photoacoustic Imaging System Based on digital micromirror device

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A Compressive Sensing Photoacoustic Imaging System Based on ...

Multi-focus non-periodic scanning method for femtosecond lasers based on DMD and galvanometer scanners[Invited]

2011 3rd International Conference on Signal Processing Systems(ICSPS 2011)

作者： Naizhang Feng Mingjian Sun Liyong Ma Department of Control Science and Engineering Harbin Institute of Technology

ISBN: (纸本)9781457701726

In this paper, a compressive sensing photoacoustic imaging scheme based on digital micromirror device(DMD) is built. In compressive sensing photoacoustic imaging, DMD is used as an optical mask. The mask is placed between a short-pulsed laser and biological tissues to realize the coded illumination. To realize the random illumination, the coded pattern of the mask should be changed for each laser pulse. Based on the DMD, random code patterns of the mask can be changed quickly by controlling a digital logical circuit. The illuminated tissue absorbs the optical energy to generate the ultrasonic waves. The generated ultrasonic waves along the same arc are compressed and detected by an unfocused ultrasonic transducer. After certain measurements, the photoacoustic image can be reconstructed by a suitable CS reconstruction algorithm.

关键词： Photoacoustic Imaging Compressive Sensing digital micromirror device Micro-electro- mechanical System

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Chinese Optics Letters 2024年第5期22卷 89-94页

作者：李华明王雨胡庆磊张卓宇吕晓华曾绍群 Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China MOE Key Laboratory for Biomedical Photonics Collaborative Innovation Center for Biomedical EngineeringSchool of Engineering SciencesHuazhong University of Science and TechnologyWuhan 430074China

Multi-focus parallel scanning can effectively increase laser fabrication ***,the conventional approach of using a spatial light modulator(SLM)to generate multi-foci and scan this fixed number of foci with galvanometer scanners can only achieve a periodic scanning trajectory due to the low switching speed of the *** we demonstrate a multifocus non-periodic scanning method for femtosecond lasers by using,instead,a fast-switching digital micromirror device(DMD)to generate a dynamic number of *** number of effective foci is quickly switched by introducing aberration to the undesired *** this way,the intensity allocated to each focus will not be affected by the number of foci,and a uniformity of 98%with different numbers of foci is achieved without adjusting the total laser ***,we validate the effectiveness of this scanning method by demonstrating corneal flap fabrication of porcine cornea in vitro.

关键词： multi-focus non-periodic trajectory digital micromirror device aberration control corneal cutting

digital generation of super-Gaussian perfect vortex beams via wavefront shaping with globally adaptive feedback

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High Power Laser Science and Engineering 2024年第1期12卷 42-51页

作者： Rui Ma Ke Hai Luo Jing Song He Wei Li Zhang Dian Yuan Fan Anderson S.L.Gomes Jun Liu International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale OptoelectronicsShenzhen UniversityShenzhenChina Institute for Advanced Study Shenzhen UniversityShenzhenChina Fiber Optics Research Centre School of Information and Communication EngineeringUniversity of Electronic Science and Technology of ChinaChengduChina Departamento de Física Universidade Federal de PernambucoRecife-PEBrazil

High-intensity vortex beams with tunable topological charges and low coherence are highly demanded in applications such as inertial confinement fusion(ICF) and optical communication. However, traditional optical vortices featuring nonuniform intensity distributions are dramatically restricted in application scenarios that require a high-intensity vortex beam owing to their ineffective amplification resulting from the intensity-dependent nonlinear effect. Here, a low-coherence perfect vortex beam(PVB) with a topological charge as high as 140 is realized based on the super-pixel wavefront-shaping technique. More importantly, a globally adaptive feedback algorithm(GAFA) is proposed to efficiently suppress the original intensity fluctuation and achieve a flat-top PVB with dramatically reduced beam speckle contrast. The GAFA-based flat-top PVB generation method can pave the way for high-intensity vortex beam generation,which is crucial for potential applications in ICF, laser processing, optical communication and optical trapping.

关键词： digital micromirror device flat-top beam orbital angular momentum perfect vortex beam random fiber laser

Real-time gradation-expressible amplitude-modulationtype electroholography based on binary-weighted computer-generated hologram

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Chinese Optics Letters 2021年第11期19卷 8-15页

作者： Ren Noguchi Kohei Suzuki Yoshiki Moriguchi Minoru Oikawa Yuichiro Mori Takashi Kakue Tomoyoshi Shimobaba Tomoyoshi Ito Naoki Takada Graduate School of Integrated Arts and Sciences Kochi UniversityKochi 780-8520Japan Research and Education Faculty Kochi UniversityKochi 780-8520Japan Graduate School of Engineering Chiba UniversityChiba 263-8522Japan

In amplitude-modulation-type electroholography, the binary-weighted computer-generated hologram(BW-CGH) facilitates the gradation-expressible reconstruction of three-dimensional(3 D) objects. To realize real-time gradation-expressible electroholography, we propose an efficient and high-speed method for calculating bit planes consisting of BW-CGHs. The proposed method is implemented on a multiple graphics processing unit(GPU) cluster system comprising 13 GPUs. The proposed BW-CGH method realizes eight-gradation-expressible electroholography at approximately the same calculation speed as that of conventional electroholography based on binary computer-generated holograms. Consequently, we were able to successfully reconstruct a real-time electroholographic 3 D video comprising approximately 180,000 points expressed in eight gradations at 30 frames per second.

关键词： gradation-expressible electroholography binary-weighted computer-generated hologram digital micromirror device computer-generated hologram

High-speed autopolarization synchronization modulation three-dimensional structured illumination microscopy

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Advanced Photonics Nexus 2024年第1期3卷 1-10页

作者： Yaning Li Ruijie Cao Wei Ren Yunzhe Fu Yiwei Hou Suyi Zhong Karl Zhanghao Meiqi Li Peng Xi Peking University College of Future TechnologyDepartment of Biomedical EngineeringBeijingChina Peking University National Biomedical Imaging CenterBeijingChina

In recent years,notable progress has been achieved in both the hardware and algorithms of structured illumination microscopy(SIM).Nevertheless,the advancement of three-dimensional structured illumination microscopy(3DSIM)has been impeded by challenges arising from the speed and intricacy of polarization *** introduce a high-speed modulation 3DSIM system,leveraging the polarizationmaintaining and modulation capabilities of a digital micromirror device(DMD)in conjunction with an electrooptic *** DMD-3DSIM system yields a twofold enhancement in both lateral(133 nm)and axial(300 nm)resolution compared to wide-field imaging and can acquire a data set comprising 29 sections of 1024 pixels×1024 pixels,with 15 ms exposure time and 6.75 s per *** versatility of the DMD-3DSIM approach was exemplified through the imaging of various specimens,including fluorescent beads,nuclear pores,microtubules,actin filaments,and mitochondria within cells,as well as plant and animal ***,polarized 3DSIM elucidated the orientation of actin ***,the implementation of diverse deconvolution algorithms further enhances 3D *** DMD-based 3DSIM system presents a rapid and reliable methodology for investigating biomedical phenomena,boasting capabilities encompassing 3D superresolution,fast temporal resolution,and polarization imaging.

关键词： digital micromirror device electro-optic modulation polarization three-dimensional structured illumination microscopy