Fringe optimization for structured illumination super-resolution microscope with digital micromirror device
作者机构：Jiangsu Key Lab of Medical Optics Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences No.88 Keling StreetSuzhou 215163P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China
出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)
年 卷 期：2019年第12卷第3期
页 面：78-86页
核心收录：
基 金：The study was funded by the National Key Technologies R&D Program of China(2018YFC0114800 and 2017YFC0109900) the Natural Science Foundation of China(NSFC)(61405238) the Natural Science Foundation of Jiangsu Province(BK20141206) the Key Technologies R&D Program of Jiangsu Province(BE2018666)
主 题：Structured illumination super-resolution digital micromirror device fringe pattern modulation depth
摘 要：Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling *** illumination can be obtained by either laser interference or projection of fringe ***,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM *** effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were *** the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil *** imaging and dynamics of subcellular organelles in live cells were also *** method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.
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