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dermal fibroblast expression of stromal cellderived factor-1 （SDF-1） promotes epidermal keratinocyte proliferation in normal and diseased skin

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Protein & Cell 2015年第12期6卷 890-903页

作者： Chunji Quan Moon Kvun Cho Yuan Shao Laurel E. Mianecki Eric Liao Daniel Perry Taihao Quan Department of Pathology Affiliated Hospital of Yanbian University Yanji 133000 China Department of Dermatology Soonchunhyang University College of Medicine Seoul Republic of Korea Hollings Cancer Center Biorepository and Tissue Analysis Resource Medical University of South Carolina Charleston SC 29425 USA Department of Dermatology University of Michigan Medical School Ann Arbor MI 48109 USA

Stromal cells provide a crucial microenvironment for overlying epithelium. Here we investigated the expression and function of a stromal cell-specific protein, stromal cell-derived factor-1 （SDF-1）, in normal human skin and in the tissues of diseased skin. Immunohistology and laser capture microdissection （LCM）-coupled quantitative real- time RT-PCR revealed that SDF-1 is constitutively and predominantly expressed in dermal stromal cells in nor- mal human skin in vivo. To our surprise, an extremely high level of SDF-1 transcription was observed in the dermis of normal human skin in vivo, evidenced by much higher mRNA expression level than type I collagen, the most abundant and highly expressed protein in human skin. SDF-1 was also upregulated in the tissues of many human skin disorders including psoriasis, basal cell carcinoma （BCC）, and squamous cell carcinoma （SCC）. Double immunostaining for SDF-1 and HSP47 （heat shock protein 47）, a marker of fibroblasts, revealed that fibroblasts were the major source of stroma-cell-derived SDF-1 in both normal and diseased skin. Functionally, SDF-1 activates the ERK （extracellular-signal-regulated kinases） pathway and functions as a mitogen to stimulate epidermalkeratinocyte proliferation. Both overexpression of SDF-1 in dermal fibroblasts and treatment with rhSDF-1 to the skin equivalent cultures significantly increased the number of keratinocyte layers and epidermal thickness. Con- versely, the stimulative function of SDF-1 on keratinocyte proliferation was nearly completely eliminated by inter- fering with CXCR4, a specific receptor of SDF-1, or by knock-down of SDF-1 in fibroblasts. Our data reveal that extremely high levels of SDF-1 provide a crucial microenvironment for epidermal keratinocyte proliferation in both physiologic and pathologic skin conditions.

关键词： SDF-1 dermal fibroblast keratinocyte,proliferation skin cancer psoriasis

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Honey exposure stimulates wound repair of human dermal fibroblasts

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Burns & Trauma 2013年第1期1卷 32-38页

作者： Elia Ranzato Simona Martinotti Bruno Burlando Dipartimento di Scienze e Innovazione Tecnologica DiSIT University of Piemonte Orientale "Amedeo Avogadro" viale T. Michel 11 15121 Alessandria Italy

Honey is widely used for treating burns, ulcers and wounds, but the mechanisms of action are poorly known and the product is mainlyused as an antimicrobial. We have examined here the wound healing properties of honey on human fi broblasts, using an in vitroscratch wound healing model. Three kinds of widely used monofl oral honeys were used, viz. acacia (Robinia pseudacacia), buckwheat(Fagopyrum sp.), and manuka (Leptospermum scoparium). Data displayed an increased wound healing activity in fi broblasts, butwith diff erent effi ciency and mechanisms of action among honeys. The eff ects of acacia and buckwheat emerged in both scratchwound and chemotaxis assays, while the eff ect of manuka was signifi cant but lower. The use of inhibitors indicated on the wholean essential role of cytosolic calcium, an important role of ERK and p38, and a secondary role of PI3K. Acacia and buckwheat,but not manuka, induced signifi cant increases in the release of interleukin-4 (IL-4), IL-6, and IL-8, indicating a correlation betweeninterleukin upregulation and wound closure effi ciency. This is consistent with our previous fi ndings suggesting a higher ability ofacacia and buckwheat to activate keratinocyte reepithelialization, with respect to manuka honey. In conclusion, our data indicatethat acacia and buckwheat honeys are particularly effi cient in facilitating fi broblast wound closure activities, suggesting newtherapeutic possibilities for this natural product.

关键词： Honey wound healing interleukins dermal fibroblast

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In vitro antioxidant and wound healing activity of Sargassum polycystum hydroethanolic extract in fibroblasts and keratinocytes

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Asian Pacific Journal of Tropical Biomedicine 2023年第5期13卷 222-232页

作者： Wanwipha Woonnoi Furoida Moolsap Supita Tanasawet Nattakanwadee Khumpirapang Chakkapat Aenglong Wanida Sukketsiri Division of Health and Applied Sciences Faculty of SciencePrince of Songkla UniversitySongkhla 90110Thailand Laboratory Animal Service Center Faculty of SciencePrince of Songkla UniversitySongkhla 90110Thailand Department of Pharmaceutical Chemistry and Pharmacognosy Faculty of Pharmaceutical SciencesNaresuan UniversityPhitsanulok65000Thailand

Objective:To investigate the in vitro antioxidant and wound healing properties of the hydroethanolic extract of Sargassum polycystum,and elucidate the mechanism of its wound healing ***:Human dermal fibroblast and HaCaT cells were used to evaluate the proliferation by sulforhodamine B and dsDNA assay after treatment with Sargassum polycystum *** wound healing and phalloidin-rhodamine staining were employed to observe migratory activity and filopodia formation,*** blot and real-time RT-PCR assays were performed to determine the protein and gene expressions related to wound healing ***:The phytochemical analysis found a higher level of flavonoid than phenolic compound in Sargassum polycystum *** human dermal fibroblast cells,Sargassum polycystum extracts at 50 and 100μg/mL significantly increased fibroblast proliferation and the gene expressions of hyaluronic acid synthase 1(HAS1),HAS2,HAS3,collagen type 1 alpha 1 chain(COL1A1),collagen type 3 alpha 1 chain(COL3A1),and *** phosphorylation of Akt,ERK1/2,and p38 MAPK was also significantly upregulated after treatment with Sargassum polycystum ***,50 and 100μg/mL of the extracts prominently enhanced the proliferation,migration,and filopodia formation of HaCaT cells,as well as the protein levels of pFAK/FAK,pSrc/Src,pAkt/Akt,pERK1/2/ERK1/2,Rac1 and ***:Sargassum polycystum extracts show promising wound healing activities in human dermal fibroblasts and keratinocytes.

关键词： dermal fibroblast Sargassum polycystum Wound healing Antioxidant Proliferation

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