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Perlecan and synaptophysin changes in denervated skeletal muscle

Neural Regeneration Research 2012年第17期7卷 1293-1298页

作者： Kai Ma Zhifeng Huang Jianfeng Ma Longquan Shao Huiming Wang Yanliang Wang Department of Oral and Maxillofacial Surgery Affiliated Hospital of Stomatology Medical College of Zhejiang University Hangzhou 310006 Zhejiang Province China Department of Oral and Maxillofacial Surgery Affiliated Hospital of Stomatology Wenzhou Medical College Wenzhou 325027 Zhejiang Province China School of Pharmacy Wenzhou Medical College Wenzhou 325000 Zhejiang Province China Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China

The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time （1-4 weeks post-injury）, perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

关键词： perlecan synaptophysin extracellular matdx acetylcholine esterase neuromuscular junction denervated skeletal muscle sciatic nerve neural regeneration

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Role of the Notch Signaling Pathway in Fibrosis of denervated Skeletal Muscle

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Current Medical Science 2019年第3期39卷 419-425页

作者： Fei FENG Lu SHAN Jing-xiu DENG Ling-li LUO Qi-shun HUANG Department of Orthopaedics Central HospitalHuang gang 438000 China Department of Orthopaedics Suizhou Hospital Hubei University of Medicine Suizhou 430072China The 457# Hospital ofPLA Wuhan 430000China Department of Hand Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

In order to investigate the role of the Notch signaling pathway in skeletal muscle fibrosis after nerve injury, 60 Sprague-Dawley rats were selected and divided randomly into a control and two experimental groups. Group A served as controls without any treatment. Rats in groups B were injected intraperitoneally with 0.2 mL PBS and those in group C were injected intraperitoneally with 0.2 mL PBS+100 ymol/L, 0.2 mL N-[N-(3,5-difluorophenacetyl)-l-alanyl]- S-phenylglycine t-butyl ester (DAPT, a gamma-secretase inhibitor that suppresses Notch signaling) respectively, on postoperative days 1, 3, 7, 10, and 14 in a model of denervation-induced skeletal muscle fibrosis by right sciatic nerve transection. Five rats from each group were euthanized on postoperative days 1, 7, 14, and 28 to collect the right gastrocnemii, and hematoxylin and eosin (HE) staining, immunohistochemistry test, real-time PCR, and Western blotting were performed to assess connective tissue hyperplasia and fibroblast density as well as expression of Notch 1, Jagged 1, and Notch downstream molecules Hes 1 and collagen I (COL I) on day 28. There was no significant difference in HE-stained fibroblast density between group B and C on postoperative day 1. However, fibroblast density was significantly higher in group B than in group C on postoperative days 7, 14, and 28. Notch 1, Jagged 1, Hes 1, and COL I proteins in the gastrocnemius were expressed at very low levels in group A but at high levels in group B. Expression levels of these proteins were significantly lower in group C than in group B (Pdenervated skeletal muscle. Thus, it may be a new approach for treatment of fibrosis of denervated skeletal muscle.

关键词： Notch signaling pathway sciatic nerve skeletal muscle fibrosis N-[N-(3,5- difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester Notch 1 Jagged 1 Hes 1 collagen I denervated muscular atrophy

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神经递质释放中的有关蛋白

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Neuroscience Bulletin 1995年第1期19卷 45-46页

作者：宋玲陈宜张

神经递质释放中的有关蛋白ＮＩＰＳ１９９５年２月号综述了神经递质释放过程中的许多重要蛋白质的作用。这些蛋白质分属于突触囊泡膜、胞液及神经末梢质膜。在突触囊泡膜上的蛋白有：Ｓｙｎａｐｔｏｂｒｅｖｉｎ，Ｓｙｎａｐｔｏｔａｇ... 详细信息

神经递质释放中的有关蛋白ＮＩＰＳ１９９５年２月号综述了神经递质释放过程中的许多重要蛋白质的作用。这些蛋白质分属于突触囊泡膜、胞液及神经末梢质膜。在突触囊泡膜上的蛋白有：Ｓｙｎａｐｔｏｂｒｅｖｉｎ，Ｓｙｎａｐｔｏｔａｇｍｉｎ，Ｓｙｎａｐｔｏｐｈｙ－ｓｉ...

关键词： motoneuron neurotrophic factors skeletal muscle extracts denervated skeletal muscle extracts

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