Perlecan and synaptophysin changes in denervated skeletal muscle

作     者：Kai Ma Zhifeng Huang Jianfeng Ma Longquan Shao Huiming Wang Yanliang Wang 

作者机构：Department of Oral and Maxillofacial Surgery Affiliated Hospital of Stomatology Medical College of Zhejiang University Hangzhou 310006 Zhejiang Province China Department of Oral and Maxillofacial Surgery Affiliated Hospital of Stomatology Wenzhou Medical College Wenzhou 325027 Zhejiang Province China School of Pharmacy Wenzhou Medical College Wenzhou 325000 Zhejiang Province China Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2012年第7卷第17期

页      面：1293-1298页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 071003[理学-生理学] 

基　　金：supported by the National Natural Science Foundation of China No.30900300/C1002 

主　　题：perlecan synaptophysin extracellular matdx acetylcholine esterase neuromuscular junction denervated skeletal muscle sciatic nerve neural regeneration 

摘      要：The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time （1-4 weeks post-injury）, perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.