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Characterization of DNA polymerase δ from deep-sea hydrothermal vent shrimp Rimicaris exoculata

Acta Oceanologica Sinica 2021年第8期40卷 168-175页

作者： Wenlin Wu Hongyun Li Tiantian Ma Xiaobo Zhang Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae Quanzhou Normal UniversityQuanzhou 362000China College of Life Sciences Zhejiang UniversityHangzhou 310058China

DNA polymerase δ(Polδ)plays a crucial and versatile role in DNA replication and DNA repair processes.vent shrimp Rimicaris exoculata is the primary megafaunal community living in hydrothermal vents.In this study,the Polδfrom shrimp Rimicaris exoculata was cloned,expressed and characterized.The results showed that the Polδcatalytic subunit(POLD1),852 amino acids in length,shared high homology with crayfish Procambarus clarkii and shrimp Oratosquilla oratoria.The recombinant POLD1 expressed in Escherichia coli showed that the enzyme was active in a range of 20℃ to 40℃ with an optimum temperature at 25℃ and in a wide range of p H with an optimum at pH 6.0.The activities of POLD1 were significantly enhanced in the presence of Triton-X 100,Tween 20 and Mn^(2+).The K_(m)(dNTP)value of POLD1 was 4.7μmol/L.The present study would be helpful to reveal the characterization of Polδof deep-sea vent animals.

关键词： deep-sea hydrothermal vent Rimicaris exoculata DNA polymeraseδ characterization

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Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre

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Acta Oceanologica Sinica 2013年第2期32卷 42-51页

作者： WEI Manman ZHANG Rubing WANG Yuguang JI Houguo ZHENG Jia CHEN Xinhua ZHOU Hongbo School of Minerals Processing and Bioengineering Central South University Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Third Institute of Oceanography State Oceanic Administration Key Laboratory of Biometallurgy Ministry of Education

The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. Microbial communities were surveyed by denatured gradient gel electrophoresis （DGGE） and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles sepa- rated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, repre- senting two selected hydrothermal samples （19-4TVG8 and 19-4TVG11）, were constructed. Twenty-three and 32 phylotypes were identified from 166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group Ⅰ and Ⅲ, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and deep-sea Hy- drothermal vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.

关键词： microbial diversity deep-sea hydrothermal vent DGGE 16S rRNA gene clone libraries

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