Characterization of DNA polymerase δ from deep-sea hydrothermal vent shrimp Rimicaris exoculata

作     者：Wenlin Wu Hongyun Li Tiantian Ma Xiaobo Zhang Wenlin Wu;Hongyun Li;Tiantian Ma;Xiaobo Zhang

作者机构：Fujian Province Key Laboratory for the Development of Bioactive Material from Marine AlgaeQuanzhou Normal UniversityQuanzhou 362000China College of Life SciencesZhejiang UniversityHangzhou 310058China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2021年第40卷第8期

页      面：168-175页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：The National Basic Research Program of China under contract No.2015CB755903 the National Natural Science Foundation of China under contract Nos U1605214 and 31470133 the Foundation of Quanzhou Normal University under contract No.2016YYKJ16 

主　　题：deep-sea hydrothermal vent Rimicaris exoculata DNA polymeraseδ characterization 

摘      要：DNA polymerase δ(Polδ)plays a crucial and versatile role in DNA replication and DNA repair *** shrimp Rimicaris exoculata is the primary megafaunal community living in hydrothermal *** this study,the Polδfrom shrimp Rimicaris exoculata was cloned,expressed and *** results showed that the Polδcatalytic subunit(POLD1),852 amino acids in length,shared high homology with crayfish Procambarus clarkii and shrimp Oratosquilla *** recombinant POLD1 expressed in Escherichia coli showed that the enzyme was active in a range of 20℃ to 40℃ with an optimum temperature at 25℃ and in a wide range of p H with an optimum at pH *** activities of POLD1 were significantly enhanced in the presence of Triton-X 100,Tween 20 and Mn^(2+).The K_(m)(dNTP)value of POLD1 was 4.7μmol/*** present study would be helpful to reveal the characterization of Polδof deep-sea vent animals.